scholarly journals Maitikkauute-aineiden vaikutus tervasroson (Cronartium flaccidum ja Peridermium pini) rihmastoviljelmien kasvuun keinoalustoilla

2010 ◽  
Vol 2010 (2) ◽  
Author(s):  
Juha Kaitera ◽  
Heikki Nuorteva
Keyword(s):  
1992 ◽  
Vol 41 (1) ◽  
pp. 91-94 ◽  
Author(s):  
M. H. PEI ◽  
J. N. GIBBS

1999 ◽  
Vol 103 (2) ◽  
pp. 235-241 ◽  
Author(s):  
J. Kaitera ◽  
L. SeitamÄKi ◽  
J. Hantula ◽  
R. Jalkanen ◽  
T. Kurkela

1999 ◽  
Vol 77 (3) ◽  
pp. 339-347 ◽  
Author(s):  
S Moricca ◽  
A Ragazzi ◽  
K R Mitchelson

An integrated approach, based on the analysis of both molecular and morphological characters, has led to the unambiguous detection and identification of the rust hyperparasite Cladosporium tenuissimum from aeciospores of the two-needle pine rust fungi Cronartium flaccidum and Peridermium pini. Cladosporium tenuissimum was first detected from contaminated field-collected rust spores using the polymerase chain reaction (PCR) method. The similar-sized amplified DNA of the parasite was then separated from rust DNA using electrophoretic migration, reamplified separately with the nested PCR, and sequenced. Sequence comparison in the data banks enabled the hyperparasite to be recognised as a species of Cladosporium. Molecular detection was followed by conventional identification, obtained by plating rust spores on potato dextrose agar, a selective medium for rusts, since they are unable to grow on such a common substrate, and isolating the hyperparasite in pure culture. It was subsequently identified as C. tenuissimum. Traditional identification would not have been possible without guidance from the molecular data, which focused attention on the mycoparasite. Macro- and micro-scopic features of colonies are also given to help with future identification on spore sources from other geographical areas and, if this should occur, future identification on other rusts.Key words: mycoparasitism, PCR detection, traditional detection, Cladosporium tenuissimum, Cronartium flaccidum, Peridermium pini.


1988 ◽  
Vol 37 (1) ◽  
pp. 45-53 ◽  
Author(s):  
J. N. GIBBS ◽  
N. ENGLAND ◽  
R. WOLSTENHOLME

2002 ◽  
Vol 106 (2) ◽  
pp. 203-209 ◽  
Author(s):  
Jarkko Hantula ◽  
Risto Kasanen ◽  
Juha Kaitera ◽  
Salvatore Moricca

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