Determination of Amino Acids in Foods by Reversed-Phase HPLC with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and Ion Exchange Chromatography

2003 ◽  
Vol 24 (1) ◽  
pp. 69-88 ◽  
Author(s):  
Kang-Lyung Woo
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Reversed Phase ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Reversed Phase Hplc

scholarly journals Plasma amino-acid determinations by reversed-phase HPLC: Improvement of the orthophthalaldehyde method and comparison with ion exchange chromatography

1992 ◽  
Vol 14 (4) ◽  
pp. 145-149 ◽  
Author(s):  
Frédéric Ziegler ◽  
Jacques Le Boucher ◽  
Colette Coudray-Lucas ◽  
Luc Cynober
Keyword(s):  
Amino Acid ◽  
Ion Exchange ◽  
Reversed Phase ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Reversed Phase Hplc ◽  
Suitable Alternative ◽  
Free Tryptophan ◽  
Rp Hplc ◽  
Low Concentrations

RP-HPLC with automated pre-column OPA derivatization is clearly a suitable alternative for assaying physiological AA and may be particularly useful for AA present at low concentrations (free tryptophan, plasma 3-methylhistidine).

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