Determination of Free Amino-Acids in Human Ejaculate by Ion Exchange Chromatography

Nature ◽  
1962 ◽  
Vol 194 (4829) ◽  
pp. 684-686 ◽  
Author(s):  
G. KRAMPITZ ◽  
R. DOEPFMER
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography

ION EXCHANGE CHROMATOGRAPHY OF THE FREE AMINO ACIDS IN THE PLASMA OF THE NEWBORN INFANT

PEDIATRICS ◽  
1965 ◽  
Vol 36 (1) ◽  
pp. 2-13
Author(s):  
Johanne C. Dickinson ◽  
Herman Rosenblum ◽  
Paul B. Hamilton
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Newborn Infant ◽  
Free Amino Acids ◽  
Free Amino ◽  
Femoral Vein ◽  
Newborn Infants ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography

1. A micromethod for the determination of amino acids by ion exchange chromatography was applied to the quantitative determination of the free amino acids in 0.1 ml of plasma of the newborn infant. The precision of the method was in general within the range ± 5%. An interpretive analysis of a typical chromatogram is given. 2. Plasma was best stored at -68°C pending analysis; errors likely to arise on storage at -20°C and higher temperatures are pointed out. Some errors likely to arise in the preparation and storage of plasma protein free filtrates are also indicated. 3. Comparison of the free amino acids in the femoral vein plasma of 25 newborn infants was made with the literature values of the free amino acids in the plasma of adults. The differences noted were not great, but were apparently characteristic. On comparing heel puncture plasma, some amino acids were higher than in femoral vein plasma, but it was concluded that heel puncture plasma would be satisfactory for routine clinical purposes. Some possible sources of error which might explain the elevated amino acids in the heel puncture plasma were noted. 4. On comparison of newborn with 3-day-old infants, some changes in amino acid levels were noted. The changes were not great, but seemed to be characteristic and consistent. 5. A number of small peaks which have not been noted previously in plasma have been indicated on the chromatogram. They were not identified, but were mentioned to draw attention to the possibilities of hitherto unrecognized ninhydrin-positive components of plasma.

scholarly journals Free amino acids in fruit bodies of some wood destroying fungi

2014 ◽  
Vol 14 (1-2) ◽  
pp. 151-155
Author(s):  
Wiesław Tadeusiak ◽  
Eliza Balicka
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Polyporus Squamosus

Concentration of free amino acids in the following bracket fungi: <i>Climacodon septentrionalis</i> (Fr) P. Karst, <i>Hapalopilus croceus</i> (Pers. ex Fr.) Donk., <i>Laetiporusus sulphurens</i> (Bull. ex Fr.) Murill and <i>Polyporus squamosus</i> Huds ex Fr., were determined by ion-exchange chromatography.

AMINO ACID COMPOSITION OF UREDOSPORES OF PUCCINIA GRAMINIS TRITICI

1965 ◽  
Vol 43 (6) ◽  
pp. 695-699 ◽  
Author(s):  
David Stefanye ◽  
Kenneth R. Bromfield
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Spore Wall ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Puccinia Graminis ◽  
Total Free Amino Acids

Uredospores of Puccinia graminis var. tritici (race 56) were analyzed quantitatively for total free amino acids and ninhydrin-positive substances by ion-exchange chromatography. Extracts of these substances were obtained by leaching the spores and by re-extracting leached spores with boiling water. Thirty-five ninhydrin-positive compounds were found and identified. The leach extract differed quantitatively from the extract obtained by boiling although both contained the same 35 substances. It is proposed that there are easily extractable ninhydrin-positive substances coating the spore wall and ninhydrin-positive substances in the protoplasm that can be extracted only with difficulty.

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