Assessment of Ribozyme Cleavage Efficiency Using Reverse Transcriptase Real-Time PCR

2000 ◽  
Vol 14 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Dagmar Klein ◽  
Melvin Denis ◽  
Camillo Ricordi ◽  
Ricardo L. Pastori
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Real Time Pcr ◽  
Cleavage Efficiency

Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR

2021 ◽  
pp. 81-86
Author(s):  
Marcus Beissner ◽  
Richard Odame Phillips ◽  
Gisela Bretzel
Keyword(s):  
16S Rrna ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Real Time Pcr

Reverse transcriptase droplet digital PCR vs reverse transcriptase quantitative real‐time PCR for serum HBV RNA quantification

2020 ◽  
Vol 92 (12) ◽  
pp. 3365-3372 ◽  
Author(s):  
Umaporn Limothai ◽  
Natthaya Chuaypen ◽  
Kittiyod Poovorawan ◽  
Watcharasak Chotiyaputta ◽  
Tawesak Tanwandee ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Real Time Pcr ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Quantitative Real Time Pcr ◽  
Rna Quantification

scholarly journals Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e50859 ◽  
Author(s):  
Jolien Vermeire ◽  
Evelien Naessens ◽  
Hanne Vanderstraeten ◽  
Alessia Landi ◽  
Veronica Iannucci ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Real Time Pcr ◽  
Accurate Method ◽  
Retroviral Vectors ◽  
Reverse Transcriptase Activity ◽  
Transcriptase Activity

scholarly journals Comparison of Real-Time PCR, Reverse Transcriptase Real-Time PCR, Loop-Mediated Isothermal Amplification, and the FDA Conventional Microbiological Method for the Detection of Salmonella spp. in Produce

2011 ◽  
Vol 77 (18) ◽  
pp. 6495-6501 ◽  
Author(s):  
Guodong Zhang ◽  
Eric W. Brown ◽  
Narjol González-Escalona
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Internal Control ◽  
Real Time Pcr ◽  
Culture Method ◽  
Isothermal Amplification ◽  
Molecular Methods ◽  
Salmonella Spp ◽  
Content Type ◽  
Loop Mediated Isothermal Amplification

ABSTRACTContamination of foods, especially produce, withSalmonellaspp. is a major concern for public health. Several methods are available for the detection ofSalmonellain produce, but their relative efficiency for detectingSalmonellain commonly consumed vegetables, often associated with outbreaks of food poisoning, needs to be confirmed. In this study, the effectiveness of three molecular methods for detection ofSalmonellain six produce matrices was evaluated and compared to the FDA microbiological detection method. Samples of cilantro (coriander leaves), lettuce, parsley, spinach, tomato, and jalapeno pepper were inoculated withSalmonellaserovars at two different levels (105and <101CFU/25 g of produce). The inoculated produce was assayed by the FDASalmonellaculture method (Bacteriological Analytical Manual) and by three molecular methods: quantitative real-time PCR (qPCR), quantitative reverse transcriptase real-time PCR (RT-qPCR), and loop-mediated isothermal amplification (LAMP). Comparable results were obtained by these four methods, which all detected as little as 2 CFU ofSalmonellacells/25 g of produce. All control samples (not inoculated) were negative by the four methods. RT-qPCR detects only liveSalmonellacells, obviating the danger of false-positive results from nonviable cells. False negatives (inhibition of either qPCR or RT-qPCR) were avoided by the use of either a DNA or an RNA amplification internal control (IAC). Compared to the conventional culture method, the qPCR, RT-qPCR, and LAMP assays allowed faster and equally accurate detection ofSalmonellaspp. in six high-risk produce commodities.

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