Semicarbazide-Sensitive Amine Oxidase and Extracellular Matrix Deposition by Smooth-Muscle Cells

2002 ◽  
Vol 2 (2) ◽  
pp. 141-150 ◽  
Author(s):  
Shannon D Langford ◽  
Margaret B Trent ◽  
Paul J Boor
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Amine Oxidase ◽  
Muscle Cells ◽  
Matrix Deposition ◽  
Extracellular Matrix Deposition

scholarly journals Inhibition of proteoglycan synthesis alters extracellular matrix deposition, proliferation, and cytoskeletal organization of rat aortic smooth muscle cells in culture.

1989 ◽  
Vol 108 (6) ◽  
pp. 2495-2505 ◽  
Author(s):  
H F Hamati ◽  
E L Britton ◽  
D J Carey
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Immunofluorescent Staining ◽  
Proteoglycan Synthesis ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Matrix Deposition ◽  
Alpha Actin

Arterial proteoglycans have been implicated in several important physiological processes ranging from lipid metabolism to regulation of smooth muscle cell growth. Vascular smooth muscle (VSM) cells are the major producers of proteoglycans in the medial layer of blood vessels. To study functional consequences of alterations in VSM proteoglycan metabolism we used 4-methylumbelliferyl-beta-D-xyloside to inhibit proteoglycan synthesis in primary and early passage cultures of rat aortic smooth muscle cells. Biochemical analysis of cultures labeled with 35SO4 showed the drug inhibited synthesis of different classes of proteoglycans by 50 to 62%. Inhibition of proteoglycan synthesis resulted in reduced accumulation of extracellular matrix, as shown by immunofluorescent staining with antibodies to chondroitin sulfate, fibronectin, thrombospondin, and laminin. There was also an inhibition of postconfluent (multilayered) growth of the smooth muscle cells, and a change in the morphology of the cells, with no apparent effect on subconfluent growth. In addition, in drug-treated cells there was a reduction in the number of cytoskeletal filaments that contained alpha-actin, the actin subtype synthesized by differentiated VSM cells. This occurred even though the total content of alpha-actin in the cells was not reduced. The effects of the inhibitor on growth and morphology could be reversed by switching the cultures to normal medium and could be prevented by growing the cells on preformed VSM extracellular matrix. These observations suggest the vascular extracellular matrix may play a role in regulating the growth and differentiation of smooth muscle cells.

Polarization, migration and homotypical interactions among prostatic smooth muscle cells in a laminin 111‐rich extracellular matrix

2020 ◽  
Author(s):  
Daniel Andrés Osório ◽  
Silvio Roberto Consonni ◽  
Aline Mara dos Santos ◽  
Hernandes F. Carvalho
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells

Abnormal Deposition of Extracellular Matrix Proteins by Cultured Smooth Muscle Cells from Human Varicose Veins

1998 ◽  
Vol 35 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Patricia Sansilvestri-Morel ◽  
Isabelle Nonotte ◽  
Marie-Pierre Fournet-Bourguignon ◽  
Alain Rupin ◽  
Jean-Noël Fabiani ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Varicose Veins ◽  
Extracellular Matrix Proteins ◽  
Muscle Cells ◽  
Matrix Proteins ◽  
Cultured Smooth Muscle Cells
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