scholarly journals Rapid Screening Test for Primary Hyperaldosteronism: Ratio of Plasma Aldosterone to Renin Concentration Determined by Fully Automated Chemiluminescence Immunoassays

2004 ◽  
Vol 50 (9) ◽  
pp. 1650-1655 ◽  
Author(s):  
Frank Holger Perschel ◽  
Rudolf Schemer ◽  
Lysann Seiler ◽  
Martin Reincke ◽  
Jaap Deinum ◽  
...  
Keyword(s):  
Screening Test ◽  
Screening Method ◽  
Plasma Renin ◽  
Preliminary Evidence ◽  
Plasma Aldosterone ◽  
Primary Hyperaldosteronism ◽  
Rapid Screening ◽  
Plasma Aldosterone Concentration ◽  
Rapid Screening Test ◽  
Edta Plasma

Abstract Background: The ratio of plasma aldosterone concentration to plasma renin activity (PAC/PRA) is the most common screening test for primary hyperaldosteronism (PHA), but it is not standardized among laboratories. We evaluated new automated assays for the simultaneous measurement of PAC and plasma renin concentration (PRC). Methods: We studied 76 healthy normotensive volunteers and 28 patients with confirmed PHA. PAC and PRC were measured immunochemically in EDTA plasma on the Nichols Advantage® chemiluminescence analyzer, and PRA was determined by an activity assay. Results: In volunteers, PAC varied from 33.3 to 1930 pmol/L, PRA from 1.13 to 19.7 ng · mL−1 · h−1 (0.215 ng · mL−1 · h−1 = 1 pmol · L−1 · s−1), and PRC from 5.70 to 116 mU/L. PAC/PRA ratios ranged from 4.35 to 494 (pmol/L)/(ng · mL−1 · h−1) and PAC/PRC ratios from 0.69 to 71.0 pmol/mU. In PHA patients, PAC ranged from 158 to 5012 pmol/L, PRA from 0.40 to 1.70 ng · mL−1 · h−1, and PRC from 0.80 to 11.7 mU/L. PAC/PRA ratios were between 298 and 6756 (pmol/L)/(ng · mL−1 · h−1) and PAC/PRC ratios between 105 and 2328 pmol/mU. Whereas PAC or PRC showed broad overlap between PHA patients and volunteers, the PAC/PRC ratio indicated distinct discrimination of these two groups at a cutoff of 71 pmol/mU. Conclusion: The PAC/PRC ratio offers several practical advantages compared with the PAC/PRA screening method. The present study offers preliminary evidence that it may be a useful screening test for PHA. Further studies are required to validate these results, especially in hypertensive cohorts.

scholarly journals Comparison of active renin concentration and plasma renin activity for the diagnosis of primary hyperaldosteronism in patients with an adrenal mass

2004 ◽  
Vol 150 (4) ◽  
pp. 517-523 ◽  
Author(s):  
N Unger ◽  
I Lopez Schmidt ◽  
C Pitt ◽  
MK Walz ◽  
T Philipp ◽  
...  
Keyword(s):  
Plasma Renin Activity ◽  
Screening Method ◽  
Plasma Renin ◽  
Renin Activity ◽  
Primary Hyperaldosteronism ◽  
Receiver Operating Curve ◽  
Adrenal Tumors ◽  
Plasma Aldosterone Concentration ◽  
Active Renin ◽  
Design And Methods

OBJECTIVE: Plasma aldosterone concentration (PAC) to plasma renin activity (PRA) ratio is an established screening test for primary hyperaldosteronism. Due to the increased recognition of adrenal incidentalomas, reliable parameters are required. Determination of active renin concentration (ARC) in contrast to PRA offers advantages with regard to processing and standardization. The present study compared PRA and ARC under random conditions to establish thresholds for the diagnosis of primary hyperaldosteronism. DESIGN AND METHODS: Fifty patients with various adrenal tumors, including ten patients with aldosterone-secreting adrenal adenomas, as well as ten hypertensive patients and 23 normotensive volunteers were studied. PAC and PRA were measured by radioimmunoassay. ARC was determined by an immunoluminometric assay. RESULTS: Receiver operating curve (ROC) analysis suggested a PAC to ARC ratio threshold of 90 ((ng/l)/(ng/l)) (sensitivity 100%, specificity 98.6%) and a ratio threshold of 62 by additional consideration of PAC > or =200 ng/l (sensitivity 100%, specificity 100%) for the diagnosis of aldosterone-secreting adrenal adenomas. CONCLUSIONS: A PAC to ARC ratio of > or =62 in patients with PAC levels > or =200 ng/l is a reliable screening method for primary hyperaldosteronism in patients with an aldosterone-producing adenoma under random conditions. Because of its advantages with regard to probe processing and its independence from endogenous angiotensinogen levels, ARC may be preferred to PRA.

Confirmation of Results of Rapid Screening Test for Aflatoxins Performed at Corn Elevator

1976 ◽  
Vol 59 (6) ◽  
pp. 1419-1421
Author(s):  
Odette L Shotwell ◽  
Gail M Shannon ◽  
Marion L Goulden
Keyword(s):  
Screening Test ◽  
Animal Feed ◽  
Screening Method ◽  
Rapid Screening ◽  
Total Aflatoxin ◽  
Corn Products ◽  
Rapid Screening Test ◽  
Aoac Method

Abstract A screening method for corn and corn products, based on a minicolumn, was modified slightly to assay 60 lots of corn at one elevator to determine whether they could be sold as animal feed. To be salable, the lots had to contain less than 20 ppb total aflatoxin. Aflatoxin levels in the lots were later determined by the official AOAC method for corn to check effectiveness of the screening. No lot had been designated for sale that contained 20 or more ppb total aflatoxin.

scholarly journals D-karyo—A New Prenatal Rapid Screening Test Detecting Submicroscopic CNVs and Mosaicism

Diagnostics ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 337
Author(s):  
Osamu Shimokawa ◽  
Masayoshi Takeda ◽  
Hiroyasu Ohashi ◽  
Akemi Shono-Ota ◽  
Mami Kumagai ◽  
...  
Keyword(s):  
Screening Test ◽  
Low Cost ◽  
Screening Method ◽  
Turnaround Time ◽  
Copy Number Variations ◽  
Rapid Screening ◽  
High Price ◽  
Chromosomal Microarray ◽  
Chromosomal Microarray Analysis ◽  
Rapid Screening Test

Chromosomal microarray analysis (CMA), recently introduced following conventional cytogenetic technology, can detect submicroscopic copy-number variations (CNVs) in cases previously diagnosed as “cytogenetically benign”. At present, rapid and accurate chromosomal analysis is required in prenatal diagnostics, but prenatal CMA is not widely used due to its high price and long turnaround time. We introduced a new prenatal screening method named digital karyotyping (D-karyo), which utilizes a preimplantation genetic test for the aneuploidy (PGT-A) platform. First, we conducted a preliminary experiment to compare the original PGT-A method to our modified method. Based on the preliminary results, we decided to implement the modified strategy without whole-genome amplification (WGA) and combined it with three analytical software packages. Next, we conducted a prospective study with 824 samples. According to the indication for invasive tests, the D-karyo positive rates were 2.5% and 5.0%, respectively, in the screening positive group with NT ≥ 3.5 mm and the group with fetal abnormalities by ultrasound. D-karyo is a breakthrough modality that can detect submicroscopic CNVs ≥ 1.0 Mb accurately in only 10.5 h for 24 samples at a low cost. Implementing D-karyo as a prenatal rapid screening test will reduce unnecessary CMA and achieve more accurate prenatal genetic testing than G-banding.

Rapid screening test for porphyrinuria

1970 ◽  
Vol 102 (2) ◽  
pp. 237-237
Author(s):  
R. M. McDonald
Keyword(s):  
Screening Test ◽  
Rapid Screening ◽  
Rapid Screening Test

A Rapid Screening Test for Detection of Poisons in Blood

Nature ◽  
1962 ◽  
Vol 193 (4822) ◽  
pp. 1298-1298 ◽  
Author(s):  
H. V. STREET
Keyword(s):  
Screening Test ◽  
Rapid Screening ◽  
Rapid Screening Test

Renin, aldosterone, electrolyte, and cortisol responses to hypoxic decompression

1977 ◽  
Vol 43 (3) ◽  
pp. 421-424 ◽  
Author(s):  
J. R. Sutton ◽  
G. W. Viol ◽  
G. W. Gray ◽  
M. McFadden ◽  
P. M. Keane
Keyword(s):  
Plasma Renin Activity ◽  
Plasma Cortisol ◽  
Acute Mountain Sickness ◽  
Plasma Renin ◽  
Plasma Aldosterone ◽  
Renin Activity ◽  
Plasma Sodium ◽  
Plasma Aldosterone Concentration ◽  
Urinary Potassium ◽  
Mountain Sickness

Responses of plasma renin activity, plasma aldosterone, plasma cortisol, and plasma electrolyte concentration and urinary electrolyte and aldosterone excretion were studied in four men during hypoxic decompression to a stimulated altitude of 4,760 m in a pressure chamber. Three of the four subjects developed significant acute mountain sickness. Plasma sodium and potassium concentrations were unchanged. No significant change in plasma renin activity was observed, but values tended to fall. Plasma aldosterone concentration was depressed while plasma cortisol was elevated and diurnal variation lost. Urinary sodium excretion was unchanged, but urinary potassium and aldosterone excretion were decreased. The decrease in plasma and urinary aldosterone and urinary potassium in the absence of change in plasma renin activity or plasma potassium is of uncertain origin. It is unlikely to be due to a decrease in adrenocorticotropin secretion since plasma cortisol rose during the same time. None of the changes could be causally implicated in the development of acute mountain sickness although the increase in plasma cortisol was greatest in the most ill.

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