scholarly journals Holo-Transcobalamin Concentration and Transcobalamin Saturation Reflect Recent Vitamin B12 Absorption Better than Does Serum Vitamin B12

2004 ◽  
Vol 50 (6) ◽  
pp. 1043-1049 ◽  
Author(s):  
Mustafa Vakur Bor ◽  
Ebba Nexø ◽  
Anne-Mette Hvas
Keyword(s):  
Vitamin B12 ◽  
Serum Vitamin ◽  
Healthy Individuals ◽  
Active Vitamin ◽  
Intraindividual Variation ◽  
Blood Samples ◽  
Normal Vitamin ◽  
Vitamin B12 Absorption ◽  
Age Range ◽  
Better Than

Abstract Background: We evaluated whether measurement of vitamin B12-saturated transcobalamin (holo-TC) concentrations or TC saturation (holo-TC:total TC) reflects active vitamin B12 absorption in healthy individuals and patients after vitamin B12 intake. Methods: We obtained blood samples from 31 healthy individuals (age range, 25–57 years) before (days −1 and 0) and after (days 1, 2, and 6) oral administration of three 9-μg doses of vitamin B12. The blood samples from seven patients (age range, 22–39 years) suspected to have decreased vitamin B12 absorption were obtained before and 1 day after the vitamin B12 intake. The blood samples were analyzed for vitamin B12, total TC, and holo-TC. The TC saturation was calculated. Results: Intraindividual variation was <13% for all measured values, as calculated from samples removed on day −1 and 0. In healthy individuals (n = 31) after intake of vitamin B12, the maximum median (range) increase (as percentages and absolute values) was in TC saturation [52 (−2% to 128)% and 0.04 (0–0.23) as a fraction], closely followed by holo-TC concentrations [39 (0–108)% and 34 (0–149) pmol/L]. All but one healthy individual had an increase of ≥15% in these markers. Serum vitamin B12 showed a smaller increase [14 (−8 to 51)% and 36 (−27 to 290) pmol/L]. After vitamin B12 intake, three patients with Crohn disease had the lowest increases in holo-TC concentration (3, 7, and 14 pmol/L) and in TC saturation (0.004, 0.01, and 0.01) among patients and 30 healthy individuals. Conclusion: Holo-TC concentrations and TC saturation reflect normal vitamin B12 absorption better than does serum vitamin B12.

scholarly journals Absorption of Cobalt60-Labeled Vitamin B12 After Subtotal Gastrectomy

Blood ◽  
1958 ◽  
Vol 13 (4) ◽  
pp. 339-347 ◽  
Author(s):  
FRITZ LOEWENSTEIN
Keyword(s):  
Urinary Excretion ◽  
Vitamin B12 ◽  
Proximal Gastrectomy ◽  
Intrinsic Factor ◽  
Subtotal Gastrectomy ◽  
Test Dose ◽  
Distal Portion ◽  
Normal Vitamin ◽  
Before And After ◽  
Vitamin B12 Absorption

Abstract Vitamin B12 absorption has been studied in patients with subtotal gastrectomy by means of the Co60 B12 urinary excretion test. Five patients tested before and after a conventional subtotal gastrectomy showed no significant change in absorption. Of 22 patients who were tested at various intervals after a conventional (distal) subtotal gastrectomy, 19 had normal vitiamin B12 absorption and three had low absorption that was corrected to normal by giving gastric juice with the test dose. Three patients who had had a proximal gastrectomy showed normal vitamin B12 absorption, indicating that intrinsic factor was being produced by the remaining distal portion of the stomach.

scholarly journals Measurement of Nutritional and Inflammatory Biomarkers in Dry Plasma Spots (DPS) (P10-097-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Neal Craft ◽  
Francisco Arredondo ◽  
Matthew Fleshman ◽  
Eleanor Brindle ◽  
Lorraine Lillis ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Storage Temperature ◽  
Nutritional Assessment ◽  
Serum Vitamin ◽  
Temperature Stability ◽  
Retinol Binding Protein ◽  
Blood Samples ◽  
Reactive Protein ◽  
Funding Sources ◽  
Gates Foundation

Abstract Objectives Conduct time and temperature stability experiments on Nutritional Biomarkers (NBs) and inflammation markers extracted from ViveBio Dry Plasma Spots (DPS). Methods Blood samples of 3 hematocrits (20, 30 and 45%) were created by combining purchased red blood cells (RBC) and sera. Blood samples of 35 µL were loaded onto ViveBio plasma separators. The underlying pads with plasma were stored at 45°, 23°, 4°, -20° and -70°C. Samples were tested on days 0, 1, 3, 7, 14, 28, 84 and 168. Ferritin, Retinol Binding Protein (RBP4), soluble Transferrin Receptors (sTfR), Thyroglobulin (Tg), α-acid Glycoprotein (AGP), and C-Reactive Protein (CRP) were measured using the Quansys Biosciences, Q-Plex Micronutrient Array (Q-Plex). Values measured in the liquid sera were used as reference values for the DPS. Red blood cell (RBC) folates and serum vitamin B12 were measured using a 96 well plate microbiological assay (ALPCO-Immundiagnostik AG). 25OH-Vitamin D3, Retinol, Methylmalonic acid (MMA) and Homocysteine (HCY) were measured using LC-MS/MS. The Q-Plex was compared at 3 independent laboratories using a group of 80 whole blood samples, non-spiked and spiked with AGP, CRP, HRP2 and sTfR. Liquid plasma and extracted plasma from ViveBio pads were generated and stored at -80°C four weeks prior to assay. Results Overall, the measured values for Ferritin, RBP4, Tg, AGP, CRP, 25OH-D, Retinol and MMA decrease with increasing storage temperature and length of storage. Ferritin and Tg exhibited the best stability at 16.8% and 8.0% over a 6 month period. Vitamin B12 exhibited very poor stability declining to below the LOQ within day 3. Most of the NBs at 1 month correlated well with liquid plasma values (r2 = 0.42 to 0.99) (AGP at 23°C, r2 = 0.428; at -70°C r2 = 0.990 Ferritin stored at 23°C, r2 = 0.854; at -70°C r2 = 0.992; Thyroglobulin at 23°C, r2 = 0.916; at -70°C r2 = 0.830). Conclusions We have demonstrated that NBs can be analyzed in low volumes of DPS generated by the ViveBio plasma separator. The Q-Plex performed reproducibly on split samples among 3 labs. These tools offer great potential to improve nutritional assessment in population studies. Funding Sources Funding provided by the Bill and Melinda Gates foundation.

scholarly journals VITAMIN B12 AND HOMOCYSTEINE LEVELS IN PATIENTS WITH NAFLD: A SYSTEMATIC REVIEW AND METANALYSIS

2021 ◽  
Author(s):  
Débora Silva COSTA ◽  
Marina P GUAHNON ◽  
Fernanda Braga SEGANFREDO ◽  
Letícia P PINTO ◽  
Cristiane V TOVO ◽  
...  
Keyword(s):  
Systematic Review ◽  
Liver Disease ◽  
Vitamin B12 ◽  
Liver Damage ◽  
Fatty Liver Disease ◽  
Serum Vitamin ◽  
Nonalcoholic Fatty Liver ◽  
Potential Marker ◽  
Vitamin B12 Absorption ◽  
Low Serum

ABSTRACT BACKGROUND: The vitamin B12 absorption can be affected in patients with nonalcoholic fatty liver disease (NAFLD), and low serum vitamin B12 levels has been related to the high homocysteine (HCY) levels and to the degree of NAFLD. OBJECTIVE: To carry out a systematic review and metanalysis of serum vitamin B12 and HCY levels in patients with NAFLD. METHODS: Original studies including serum vitamin B12 and HCY levels in humans with NAFLD were included. The searches were performed in four databases. RESULTS: 159 studies were identified, and after excluding the duplicates and non-eligible titles, eight original articles were included. Six out of eight showed higher B12 levels in NAFLD patients (404.9±136.2 pg/mL in relation to controls 353.91±117.3 pg/mL). Seven of the eight studies also showed higher HCY levels in NAFLD patients (14.2±3.44 umol/L in relation to controls 11.05±3.6 umol/L). The results for serum vitamin B12 and HCY levels were submitted to metanalysis, showing no difference in the vitamin B12 levels between patients with NAFLD and controls. However, the levels of Hcy were higher in NAFLD patients than in controls. CONCLUSION: There was no relashionship between the vitamin B12 levels and NAFLD. The levels of HCY were significantly higher in patients with NAFLD, suggesting this could be a potential marker for liver damage.

Peculiarities of vitamin B12 absorption disorders in diseases of the digestive organs

1986 ◽  
Vol 67 (2) ◽  
pp. 133-134
Author(s):  
V. V. Trusov ◽  
Ya. M. Vakhrushev ◽  
T. I. Sterkhova ◽  
B. P. Gagarin ◽  
B. I. Martynov
Keyword(s):  
Chronic Hepatitis ◽  
Small Bowel ◽  
Vitamin B12 ◽  
Digestive System ◽  
Bowel Resection ◽  
Chronic Atrophic Gastritis ◽  
Small Bowel Resection ◽  
Control Group ◽  
Healthy Individuals ◽  
Vitamin B12 Absorption

We studied the process of vitamin B12 absorption in 184 patients with various diseases of the digestive system. 57 patients suffered from chronic atrophic gastritis, 42 - cirrhosis of the liver, 28 - chronic hepatitis; 12 patients underwent small bowel resection (more than 1 m), 45 - gastric resections of various types. The control group consisted of 25 healthy individuals aged 22 to 47 years.

scholarly journals Assessment of Vitamin B12 Absorption Based on the Accumulation of Orally Administered Cyanocobalamin on Transcobalamin

2010 ◽  
Vol 56 (3) ◽  
pp. 432-436 ◽  
Author(s):  
Tore Forsingdal Hardlei ◽  
Anne Louise Mørkbak ◽  
Mustafa Vakur Bor ◽  
Lynn B Bailey ◽  
Anne-Mette Hvas ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Reference Interval ◽  
Relative Increase ◽  
Serum Samples ◽  
Intraindividual Variation ◽  
Additional Increase ◽  
Absolute Increase ◽  
Subtilisin Carlsberg ◽  
Healthy Donors ◽  
Vitamin B12 Absorption

Abstract Background: Vitamin B12, or cobalamin (Cbl), is absorbed in the intestine and transported to the cells bound to transcobalamin (TC). We hypothesize that cyanocobalamin (CNCbl) is absorbed unchanged, thereby allowing measurement of the complex of CNCbl bound to TC (TC–CNCbl) to be used for studying the absorption of the vitamin. Methods: TC was immunoprecipitated from serum samples obtained from healthy donors at baseline and at 24 h after oral administration of three 9-μg CNCbl doses over 1 day. Cbl was released by treatment with subtilisin Carlsberg. The different forms of Cbl were isolated by HPLC and subsequently quantified with an ELISA-based Cbl assay. Results: At baseline, the median TC–CNCbl concentration was 1 pmol/L (range, 0–10 pmol/L); the intraindividual variation (SD) was 1.6 pmol/L (n = 31). After CNCbl administration, the TC–CNCbl concentration increased significantly (P = 0.0003, paired t-test), whereas no major changes were observed in any of the other Cbl forms bound to TC (n = 10). Only a moderate additional increase in TC–CNCbl was observed with prolonged (5 days) CNCbl administration (n = 10). We designed an absorption test based on measuring TC–CNCbl at baseline and 24 h after CNCbl intake and established a reference interval for the increase in TC–CNCbl (n = 78). The median absolute increase was 23 pmol/L (range, 6–64 pmol/L), and the relative increase was >3-fold. Conclusions: Our data demonstrate that CNCbl is absorbed unchanged and accumulates on circulating TC. We suggest that measuring TC–CNCbl will improve the assessment of vitamin B12 absorption.

scholarly journals Estimation of the level of homocysteine and vitamin B12 in serum of patients with hypothyroidism

2019 ◽  
Vol 24 (1) ◽  
pp. 70
Author(s):  
Asala Adnan Otla ◽  
Nadia Ahmed Saleh
Keyword(s):  
Vitamin B12 ◽  
Homocysteine Level ◽  
Control Group ◽  
Healthy Individuals ◽  
Blood Samples

In this study, homocysteine and vitamin B12 levels were measured in patients with hypothyroidism in Salahdin Governorate. The study was conducted at the Tigris Educational Hospital in Tikrit. Blood  samples were taken from patients with hypothyroidism  (40 cases) with thyroid deficiency and 30 healthy (non-infected) subjects. Their ages ranged between 25-35 years. The study found that there was a significant increase p<0.05 in the homocysteine level compared to the healthy Individuals as control group. The study also showed a significant decrease in the level of vitamin  B12 for patients p <0.05 compared to healthy individuals .   http://dx.doi.org/10.25130/tjps.24.2019.011

CobaSorb, a New Non-Radioactive Vitamin B12 Absorption Test.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3749-3749
Author(s):  
Anne-Mette Hvas ◽  
Louise Morkbak ◽  
Ebba Nexo
Keyword(s):  
Vitamin B12 ◽  
Vitamin B12 Deficiency ◽  
Oral Intake ◽  
Positive Association ◽  
Absorption Test ◽  
Healthy Individuals ◽  
Significant Positive Association ◽  
B12 Deficiency ◽  
Vitamin B12 Absorption ◽  
Baseline Levels

Abstract After diagnosing vitamin B12 deficiency, the cause of the deficiency has to be clarified. For many years Schillings test was used for this purpose. However, Schillings test has almost disappeared from the marked. Recently, a new vitamin B12 absorption test, CobaSorb, was introduced. CobaSorb is based on oral intake of non-radioactive vitamin B12 followed by measurement of an increase in holotranscobalamin (holoTC) 24 hours later. In the present study, we further explored the design of CobaSorb to identify the best marker for reflection of vitamin B12 absorption and to determine the duration of the test. Seventy-eight healthy individuals (age 21–81 years, 40 males) were included and three oral doses of 9 microgram vitamin B12 per day were administered for 5 successive days. Non-fasting blood samples were collected on days 1 to 5 before administration of vitamin B12 and in the morning day 8. Plasma cobalamins were assayed on the Advia Centaur (Bayer A/S, Germany), and holoTC was measured by an in-house sandwish ELISA after removal of the apoTC with vitamin B12 coated beads. Baseline levels were 151–528 pmol/L for plasma cobalamins and 19–33 pmol/L for holoTC. We found a significant positive association between age and baseline holoTC (p=0.03), whereas no association was found between baseline plasma cobalamins and age (p=0.55). Baseline levels were not associated with sex. Because a vitamin B12 absorption test is relevant only in individuals with borderline or low levels of holoTC or plasma cobalamins, we analysed in detail the pattern of absorption in those having baseline levels below the 75% percentiles. We used a change greater than 2×CVday-to-day in holoTC (22%) and plasma cobalamins (12%) to indicate a change caused by absorption of vitamin B12. Among individuals with a baseline holoTC below the 75% percentile (<75 pmol/L, n=57), the increase in holoTC from baseline (day 1) to day 3 was > 22% for 56 (98%). Only 41 (72%) of the individuals with baseline plasma cobalamins below the 75% percentile (<335 pmol/L, n=57) had an increase in plasma cobalamins >12% from day 1 to day 3. We concluded that in healthy individuals with baseline holoTC <75 pmol/L, vitamin B12 absorption was well reflected by an increase in holoTC-but not plasma cobalamins-after 2 days administration of oral vitamin B12. We suggest that future use of CobaSorb is done based on this design.

Use of soluble glass boluses containing Cu, Co and Se in the prevention of trace-element deficiencies in goats

1988 ◽  
Vol 110 (1) ◽  
pp. 155-158 ◽  
Author(s):  
G. P. Zervas
Keyword(s):  
Trace Elements ◽  
Vitamin B12 ◽  
Trace Element ◽  
Serum Vitamin ◽  
Experimental Period ◽  
The Other ◽  
Blood Samples ◽  
The Third ◽  
Soluble Glass ◽  
Plasma Copper

SummaryTwo experiments were carried out to examine the value of copper, cobalt and selenium contained in soluble glass boluses as a means of providing supplementary trace elements to goats.In the first experiment 36 purebred Toggenburg goats were used and divided into two groups. One group was treated with soluble glass boluses while the other was left as control.In the second experiment 46 Saanen half-bred goats were used, divided into three groups. The first group was treated with soluble glass boluses, the second was treated with selenium injectable compound and the third was left as control.Blood samples were collected on four occasions, during an experimental period of almost 12 months and plasma copper, serum vitamin B12 and blood selenium concentrations were determined. All those measurements showed significant increases for about a year, and were due to treatment. The advantages of glass boluses were not shared by the selenium injectable compound.

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