scholarly journals Characterization of Metabolically Quiescent Leishmania Parasites in Murine Lesions Using Heavy Water Labeling

2015 ◽  
Vol 11 (2) ◽  
pp. e1004683 ◽  
Author(s):  
Joachim Kloehn ◽  
Eleanor C. Saunders ◽  
Sean O’Callaghan ◽  
Michael J. Dagley ◽  
Malcolm J. McConville
Keyword(s):  
Heavy Water ◽  
Leishmania Parasites

scholarly journals Isolation and Molecular Typing of Leishmania infantum from Phlebotomus perfiliewi in a Re-Emerging Focus of Leishmaniasis, Northeastern Italy

2019 ◽  
Vol 7 (12) ◽  
pp. 644
Author(s):  
Mattia Calzolari ◽  
Elena Carra ◽  
Gianluca Rugna ◽  
Paolo Bonilauri ◽  
Federica Bergamini ◽  
...  
Keyword(s):  
Leishmania Infection ◽  
Sand Flies ◽  
Repetitive Region ◽  
Microsatellite Typing ◽  
Increasing Trend ◽  
Northeastern Italy ◽  
Emilia Romagna Region ◽  
Phlebotomus Perfiliewi ◽  
Leishmania Parasites

Visceral leishmaniasis (VL) caused by Leishmania (L.) infantum is a public health threat in the Emilia-Romagna region, northeastern Italy, but its epidemiology has not been fully elucidated in this area. The objective of this study was to characterize Leishmania infection in sand flies collected in a re-emerging focus of VL in the Bologna province. During the summer of 2016, 6114 sand flies were collected, identified, and tested for Leishmania detection. Of the identified sand flies, 96.5% were Phlebotomus (P.) perfiliewi and 3.5% were P. perniciosus. Detected parasites were characterized by biomolecular methods (multilocus microsatellite typing and characterization of repetitive region on chromosome 31), and quantified by real-time PCR. The prevalence of Leishmania infection in individually-tested P. perfiliewi sand flies varied from 6% to 10% with an increasing trend during the season. Promastigotes of L. infantum were isolated by dissection in one P. perfiliewi female; the isolated strain (Lein-pw) were closely related to Leishmania parasites from VL cases in northeastern Italy, but differed from strains isolated in dogs from the same area. Our findings strongly support the vector status of P. perfiliewi for human VL in the study area.

scholarly journals Molecular and functional characterization of two malic enzymes from Leishmania parasites

2018 ◽  
Vol 219 ◽  
pp. 67-76 ◽  
Author(s):  
Lucila Giordana ◽  
Máximo Hernán Sosa ◽  
Alejandro E. Leroux ◽  
Elkin F. Rodas Mendoza ◽  
Patricia Petray ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Leishmania Parasites

scholarly journals Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e109773 ◽  
Author(s):  
Imen Mkada–Driss ◽  
Ramzi Lahmadi ◽  
Ahmed S. Chakroun ◽  
Chiraz Talbi ◽  
Souheila Guerbouj ◽  
...  
Keyword(s):  
Rapd Markers ◽  
Leishmania Parasites

scholarly journals Characterization of Leishmania parasites isolated from provinces of the Islamic Republic of Iran

2002 ◽  
Vol 8 (2-3) ◽  
pp. 338-344
Author(s):  
H. Motazedian ◽  
B. Noamanpoor ◽  
S. Ardehali
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Cutaneous Lesion ◽  
Recent Change ◽  
Islamic Republic ◽  
Cutaneous Lesions ◽  
Rapd Pcr ◽  
Islamic Republic Of Iran ◽  
Polymerase Chain ◽  
Leishmania Parasites

Leishmania parasites isolated in the Islamic Republic of Iran were studied by a random amplified polymorphic DNA polymerase chain reaction [RAPD-PCR]. Of 82 isolates, 80 were from cutaneous lesions, 1 from a human throat lesion and 1 from a dog. Of these, 42 isolates were L. tropica, 36 were L. major and 2 were L. infantum. There were 2 unidentified isolates [from the throat lesion and a cutaneous lesion] and these demonstrated 52% and 48% similarity with L. tropica and L. infantum. Both L. tropica and L. major were isolated from four provinces indicating a recent change in the epidemiology of cutaneous leishmaniasis. L. tropica was isolated from three provinces; L. major from one province. L. infantum was isolated from a human cutaneous lesion and from a dog in Bushehr province.

scholarly journals Co-Circulation of Phleboviruses and Leishmania Parasites in Sand Flies from a Single Site in Italy Monitored between 2017 and 2020

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1660
Author(s):  
Mattia Calzolari ◽  
Giuseppe Romeo ◽  
Emanuele Callegari ◽  
Paolo Bonilauri ◽  
Chiara Chiapponi ◽  
...  
Keyword(s):  
Virus Isolate ◽  
Simultaneous Detection ◽  
Single Site ◽  
Sand Flies ◽  
Toscana Virus ◽  
Support Measures ◽  
Phlebotomus Perfiliewi ◽  
Partial Overlap ◽  
Leishmania Parasites

Sand flies transmit Leishmania infantum, which is responsible for causing leishmaniasis, as well as many phleboviruses, including the human pathogenic Toscana virus. We screened sand flies collected from a single site between 2017 and 2020 for the presence of both phleboviruses and Leishmania. The sand flies were sampled with attractive carbon dioxide traps and CDC light traps between May and October. We collected more than 50,000 sand flies; 2826 were identified at the species level as Phlebotomus perfiliewi (98%) or Phlebotomus perniciosus (2%). A total of 16,789 sand flies were tested in 355 pools, and phleboviruses were found in 61 pools (6 Toscana virus positive pools, 2 Corfou virus positive pools, 42 Fermo virus positive pools, and 7 Ponticelli virus positive pools, and 4 unidentified phlebovirus positive pools). Leishmania was found in 75 pools and both microorganisms were detected in 16 pools. We isolated nine phleboviruses from another 2960 sand flies (five Ponticelli viruses and for Fermo viruses), not tested for Leishmania; the complete genome of a Fermo virus isolate was sequenced. The simultaneous detection in space and time of the Fermo virus and L. infantum is evidence that supports the co-circulation of both microorganisms in the same location and partial overlap of their cycles. A detailed characterization of the epidemiology of these microorganisms will support measures to limit their transmission.

scholarly journals Synthesis and characterization of some 1, 5-benzodiazepine derivatives from Chalcones and their use as scavengers for some heavy metals in Environmental Systems.

2022 ◽  
Vol 961 (1) ◽  
pp. 012094
Author(s):  
Ahmad Moulood Taha ◽  
Malath Khalaf Rasheed
Keyword(s):  
Heavy Metals ◽  
Heavy Water ◽  
Basic Medium ◽  
Synthesis And Characterization ◽  
Microwave Method ◽  
Benzodiazepine Derivatives ◽  
The Stability ◽  
Laser Rays ◽  
Derivatives Of

Abstract Chalcones (A1-A4) compounds were prepared by reacting an aldehyde (Cinnamaldehyde or terphthaldehyde) with a ketone (4-aminoacetophenone or acetylacetone) in the presence of 30% NaOH as a catalyst and ethanol as a solvent. Derivatives of 1,5-benzodiazepine (A5-A12) were prepared by reacting these prepared chalcones with orthophenylene diamine or 4-methylorthophenylene diamine in a basic medium of sodium hydroxide 10% using the microwave method. Some prepared compounds were used as scavengers of some heavy metals (iron and lead) from heavy water in ecosystems, as the results indicated the ability of these compounds to reduce or withdraw these elements from heavy water. The stability of these compounds was also tested against laser rays, as they showed resistance and stability towards laser rays.

SEM and EDS Characterization of Titanium Cathodes Before and After Electrolysis in Heavy Water

1999 ◽  
Vol 5 (S2) ◽  
pp. 598-599
Author(s):  
J. Warner ◽  
J. Dash
Keyword(s):  
Heavy Water ◽  
Titanium Foil ◽  
Deionized Water ◽  
Acid Electrolyte ◽  
Sulfuric Acid Electrolyte ◽  
Before And After ◽  
Gentle Agitation ◽  
Series Of Experiments ◽  
Experiment Analysis

A series of experiments were conducted with titanium cathodes and platinum anodes electrolyzed in heavy water-sulfuric acid electrolyte in closed cells. Each of the cathodes in these experiments was cut from the same titanium foil (99.99% Ti). Each cathode underwent a pre-experiment and a post-experiment SEM (ISI-SS40) and EDS (LINK AN10000) analysis. Of the eight experimental cathodes, three showed post-experimental evidence of localized chemical changes. This is consistent with other findings from this lab. Results obtained on one of these cathodes are presented here.This cathode was electrolyzed for 115 hours at 0.40A (current density about 2A/cm2). The cathode was ultrasonically cleaned in deionized water for five minutes prior to the pre experiment analysis. Fig. 1 shows the smooth surface of the cathode before electrolysis. No other elements besides titanium were found. After electrolysis in 19.4g D2O +l.0g H2SO4 the cathode was removed from the cell and rinsed by gentle agitation in deionized water for several minutes.

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