scholarly journals Insights into the phosphatidylcholine and phosphatidylethanolamine biosynthetic pathways in Leishmania parasites and characterization of a choline kinase from Leishmania infantum

2017 ◽  
Vol 213 ◽  
pp. 45-54 ◽  
Author(s):  
Sergio A. Pulido ◽  
Victoria H. Nguyen ◽  
Juan F. Alzate ◽  
David L. Cedeño ◽  
Monika A. Makurath ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Choline Kinase ◽  
Biosynthetic Pathways ◽  
Leishmania Parasites

scholarly journals Isolation and Molecular Typing of Leishmania infantum from Phlebotomus perfiliewi in a Re-Emerging Focus of Leishmaniasis, Northeastern Italy

2019 ◽  
Vol 7 (12) ◽  
pp. 644
Author(s):  
Mattia Calzolari ◽  
Elena Carra ◽  
Gianluca Rugna ◽  
Paolo Bonilauri ◽  
Federica Bergamini ◽  
...  
Keyword(s):  
Leishmania Infection ◽  
Sand Flies ◽  
Repetitive Region ◽  
Microsatellite Typing ◽  
Increasing Trend ◽  
Northeastern Italy ◽  
Emilia Romagna Region ◽  
Phlebotomus Perfiliewi ◽  
Leishmania Parasites

Visceral leishmaniasis (VL) caused by Leishmania (L.) infantum is a public health threat in the Emilia-Romagna region, northeastern Italy, but its epidemiology has not been fully elucidated in this area. The objective of this study was to characterize Leishmania infection in sand flies collected in a re-emerging focus of VL in the Bologna province. During the summer of 2016, 6114 sand flies were collected, identified, and tested for Leishmania detection. Of the identified sand flies, 96.5% were Phlebotomus (P.) perfiliewi and 3.5% were P. perniciosus. Detected parasites were characterized by biomolecular methods (multilocus microsatellite typing and characterization of repetitive region on chromosome 31), and quantified by real-time PCR. The prevalence of Leishmania infection in individually-tested P. perfiliewi sand flies varied from 6% to 10% with an increasing trend during the season. Promastigotes of L. infantum were isolated by dissection in one P. perfiliewi female; the isolated strain (Lein-pw) were closely related to Leishmania parasites from VL cases in northeastern Italy, but differed from strains isolated in dogs from the same area. Our findings strongly support the vector status of P. perfiliewi for human VL in the study area.

Characterization of Both Polyunsaturated Fatty Acid Biosynthetic Pathways in Schizochytrium sp.

Lipids ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 621-630 ◽  
Author(s):  
J. Casey Lippmeier ◽  
Kristine S. Crawford ◽  
Carole B. Owen ◽  
Angie A. Rivas ◽  
James G. Metz ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Biosynthetic Pathways

scholarly journals Structural and Enzymatic Characterization of the Choline Kinase LicA from Streptococcus pneumoniae

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120467 ◽  
Author(s):  
Lei Wang ◽  
Yong-Liang Jiang ◽  
Jing-Ren Zhang ◽  
Cong-Zhao Zhou ◽  
Yuxing Chen
Keyword(s):  
Streptococcus Pneumoniae ◽  
Choline Kinase ◽  
Enzymatic Characterization

scholarly journals In vitro and in vivo resistance of Leishmania infantum to meglumine antimoniate: a study of 37 strains collected from patients with visceral leishmaniasis.

1997 ◽  
Vol 41 (4) ◽  
pp. 827-830 ◽  
Author(s):  
F Faraut-Gambarelli ◽  
R Piarroux ◽  
M Deniau ◽  
B Giusiano ◽  
P Marty ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Strongly Correlated ◽  
Duration Of Treatment ◽  
In Vitro Sensitivity ◽  
Sensitivity Tests ◽  
Leishmania Parasites ◽  
Immunocompetent Patients

Primary and secondary unresponsiveness to meglumine has long been described in human visceral leishmaniasis. However, no studies have been performed to elucidate if these therapeutic failures were due to strain variability in meglumine sensitivity or were related to host factors. We have studied the in vitro sensitivity of 37 strains of Leishmania infantum isolated from 23 patients (11 human immunodeficiency virus-infected and 12 immunocompetent patients) with visceral leishmaniasis. Sensitivity tests were performed by infecting murine macrophages with Leishmania parasites and culturing them in medium containing different concentrations of meglumine. For each test we calculated a 50% effective dose (ED50) corresponding to the meglumine concentration at which 50% of the Leishmania parasites survived. In vitro results were strongly correlated to immediate clinical outcome. All strains requiring an ED50 of >70 microg/ml were related to therapeutic failures, whereas all strains requiring an ED50 of <40 microg/ml corresponded to an initial efficiency of meglumine. Among those patients who were initially improved, relapses occurred in all immunocompromised patients and in most immunocompetent patients who had a short duration of treatment (15 days). Finally, we found that in vitro sensitivity of strains decreased progressively in relapsing patients treated with meglumine. Consequently, the physician may be encouraged to alternate meglumine with other treatments such as amphotericin B or pentamidine, especially in the case of relapsing patients.

scholarly journals Genomic and Molecular Characterization of Miltefosine Resistance in Leishmania infantum Strains with Either Natural or Acquired Resistance through Experimental Selection of Intracellular Amastigotes

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0154101 ◽  
Author(s):  
Annelies Mondelaers ◽  
Maria P. Sanchez-Cañete ◽  
Sarah Hendrickx ◽  
Eline Eberhardt ◽  
Raquel Garcia-Hernandez ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Leishmania Infantum ◽  
Acquired Resistance ◽  
Intracellular Amastigotes ◽  
Selection Of

scholarly journals Is the Prevalence of Leishmania infantum Linked to Breeds in Dogs? Characterization of Seropositive Dogs in Ibiza

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2579
Author(s):  
Maria Edo ◽  
Pablo Jesús Marín-García ◽  
Lola Llobat
Keyword(s):  
Immune Response ◽  
Leishmania Infantum ◽  
Protozoan Parasite ◽  
Disease Stage ◽  
Doberman Pinscher ◽  
Th2 Immune Response ◽  
Mediterranean Island ◽  
Antibody Titers ◽  
The Relationship

Leishmaniosis is an important zoonotic protozoan disease primarily spread to the Mediterranean region by Leishmania infantum, the predominant protozoan species, which accounts for the majority of cases. Development of disease depends on the immune response of the definitive host and, predictably, their genetic background. Recent studies have revealed breed-typical haplotypes that are susceptible to the spread of the protozoan parasite. The objective of this study was to analyze the prevalence of leishmaniosis on a Mediterranean island and determine the relationship between disease prevalence and breed. In addition, information on seropositive animals was recorded to characterize animals affected by the disease. To study the prevalence, a total of 3141 dogs were analyzed. Of these, the 149 infected animals were examined for age, sex, antibody titer, and disease stage. We observed a prevalence of 4.74%, which varied between breeds (p < 0.05). The Doberman Pinscher and Boxer breeds had the highest prevalence of leishmaniosis. Significant differences were observed between breeds with common ancestors, emphasizing the important genetic component. Finally, regarding the characterization of seropositive animals, the distribution is similar to other studies. We discovered a relationship (p < 0.05) between the number of antibody titers and the clinical disease stage, which was also present in Leishmania infantum, suggesting that the development of the disease depends on the humoral or Th2 immune response with ineffective antibodies.

Genetic and clinical characterization of canine leishmaniasis caused by Leishmania (Leishmania) infantum in northeastern Argentina

Acta Tropica ◽  
2015 ◽  
Vol 150 ◽  
pp. 218-223 ◽  
Author(s):  
Paola A. Barroso ◽  
M.Cecilia Nevot ◽  
Carlos L. Hoyos ◽  
Fabricio M. Locatelli ◽  
Juan J. Lauthier ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Canine Leishmaniasis ◽  
Northeastern Argentina

Characterization of a protein with unknown function (LinJ.30.3360) in Leishmania amazonensis and Leishmania infantum

2021 ◽  
Vol 221 ◽  
pp. 108048
Author(s):  
Andreza Geisiane Maia Souza ◽  
Ivana Helena Rocha Oliveira ◽  
Aliani Moura Fonseca ◽  
Thamires Queiroz-Oliveira ◽  
Érica S. Martins-Duarte ◽  
...  
Keyword(s):  
Unknown Function ◽  
Leishmania Infantum ◽  
Leishmania Amazonensis
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