scholarly journals Structural and Functional Studies of Nonstructural Protein 2 of the Hepatitis C Virus Reveal Its Key Role as Organizer of Virion Assembly

2010 ◽  
Vol 6 (12) ◽  
pp. e1001233 ◽  
Author(s):  
Vlastimil Jirasko ◽  
Roland Montserret ◽  
Ji Young Lee ◽  
Jérôme Gouttenoire ◽  
Darius Moradpour ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nonstructural Protein ◽  
Virion Assembly ◽  
Functional Studies ◽  
Nonstructural Protein 2

scholarly journals Inhibitors of Endoplasmic Reticulum α-Glucosidases Potently Suppress Hepatitis C Virus Virion Assembly and Release

2010 ◽  
Vol 55 (3) ◽  
pp. 1036-1044 ◽  
Author(s):  
Xiaowang Qu ◽  
Xiaoben Pan ◽  
Jessica Weidner ◽  
Wenquan Yu ◽  
Dominic Alonzi ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nonstructural Protein ◽  
Hcv Rna ◽  
Virion Assembly ◽  
Subsequent Degradation ◽  
Important Host ◽  
Antiviral Targets ◽  
Sugar Derivatives

ABSTRACTα-Glucosidases I and II are endoplasmic reticulum-resident enzymes that are essential for N-linked glycan processing and subsequent proper folding of glycoproteins. In this report, we first demonstrate that downregulation of the expression of α-glucosidase I, II, or both in Huh7.5 cells by small hairpin RNA technology inhibited the production of hepatitis C virus (HCV). In agreement with the essential role of α-glucosidases in HCV envelope glycoprotein processing and folding, treatment of HCV-infected cells with a panel of imino sugar derivatives, which are competitive inhibitors of α-glucosidases, did not affect intracellular HCV RNA replication and nonstructural protein expression but resulted in the inhibition of glycan processing and subsequent degradation of HCV E2 glycoprotein. As a consequence, HCV virion assembly and secretion were inhibited. In searching for imino sugars with better antiviral activity, we found that a novel imino sugar, PBDNJ0804, had a superior ability to inhibit HCV virion assembly and secretion. In summary, we demonstrated that glucosidases are important host factor-based antiviral targets for HCV infection. The low likelihood of drug-resistant virus emergence and potent antiviral efficacy of the novel glucosidase inhibitor hold promise for its development as a therapeutic agent for the treatment of chronic hepatitis C.

Cyclosporine A inhibits hepatitis C virus nonstructural protein 2 through cyclophilin A

Hepatology ◽  
2009 ◽  
Vol 50 (5) ◽  
pp. 1638-1645 ◽  
Author(s):  
Sandra Ciesek ◽  
Eike Steinmann ◽  
Heiner Wedemeyer ◽  
Michael P. Manns ◽  
Johann Neyts ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cyclosporine A ◽  
Nonstructural Protein ◽  
Cyclophilin A ◽  
Nonstructural Protein 2

Analysis of N-terminal processing of hepatitis C virus nonstructural protein 2.

1994 ◽  
Vol 68 (4) ◽  
pp. 2731-2734 ◽  
Author(s):  
H Mizushima ◽  
M Hijikata ◽  
Y Tanji ◽  
K Kimura ◽  
K Shimotohno
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nonstructural Protein ◽  
Nonstructural Protein 2

scholarly journals Structural and Functional Characterization of Nonstructural Protein 2 for Its Role in Hepatitis C Virus Assembly

2008 ◽  
Vol 283 (42) ◽  
pp. 28546-28562 ◽  
Author(s):  
Vlastimil Jirasko ◽  
Roland Montserret ◽  
Nicole Appel ◽  
Anne Janvier ◽  
Leah Eustachi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Assembly ◽  
Nonstructural Protein ◽  
Functional Characterization ◽  
Nonstructural Protein 2

scholarly journals Role for TBC1D20 and Rab1 in Hepatitis C Virus Replication via Interaction with Lipid Droplet-Bound Nonstructural Protein 5A

2012 ◽  
Vol 86 (12) ◽  
pp. 6491-6502 ◽  
Author(s):  
I. Nevo-Yassaf ◽  
Y. Yaffe ◽  
M. Asher ◽  
O. Ravid ◽  
S. Eizenberg ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Lipid Droplet ◽  
Nonstructural Protein ◽  
Nonstructural Protein 5A
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