scholarly journals Structural and Functional Characterization of Nonstructural Protein 2 for Its Role in Hepatitis C Virus Assembly

2008 ◽  
Vol 283 (42) ◽  
pp. 28546-28562 ◽  
Author(s):  
Vlastimil Jirasko ◽  
Roland Montserret ◽  
Nicole Appel ◽  
Anne Janvier ◽  
Leah Eustachi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Assembly ◽  
Nonstructural Protein ◽  
Functional Characterization ◽  
Nonstructural Protein 2

scholarly journals Structural and Functional Studies of Nonstructural Protein 2 of the Hepatitis C Virus Reveal Its Key Role as Organizer of Virion Assembly

2010 ◽  
Vol 6 (12) ◽  
pp. e1001233 ◽  
Author(s):  
Vlastimil Jirasko ◽  
Roland Montserret ◽  
Ji Young Lee ◽  
Jérôme Gouttenoire ◽  
Darius Moradpour ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nonstructural Protein ◽  
Virion Assembly ◽  
Functional Studies ◽  
Nonstructural Protein 2

scholarly journals Immunogenicity and functional characterization of Leishmania-derived hepatitis C virus envelope glycoprotein complex

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Katarzyna Grzyb ◽  
Anna Czarnota ◽  
Agnieszka Brzozowska ◽  
Anna Cieślik ◽  
Łukasz Rąbalski ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Functional Characterization ◽  
Virus Envelope ◽  
Glycoprotein Complex

Cyclosporine A inhibits hepatitis C virus nonstructural protein 2 through cyclophilin A

Hepatology ◽  
2009 ◽  
Vol 50 (5) ◽  
pp. 1638-1645 ◽  
Author(s):  
Sandra Ciesek ◽  
Eike Steinmann ◽  
Heiner Wedemeyer ◽  
Michael P. Manns ◽  
Johann Neyts ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cyclosporine A ◽  
Nonstructural Protein ◽  
Cyclophilin A ◽  
Nonstructural Protein 2

scholarly journals Functional Characterization of Intracellular and Secreted Forms of a Truncated Hepatitis C Virus E2 Glycoprotein

2000 ◽  
Vol 74 (2) ◽  
pp. 702-709 ◽  
Author(s):  
Mike Flint ◽  
Jean Dubuisson ◽  
Catherine Maidens ◽  
Richard Harrop ◽  
Geoffrey R. Guile ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Functional Characterization ◽  
Significant Proportion ◽  
Biologically Active ◽  
Surface Glycoprotein ◽  
Future Design ◽  
E2 Glycoprotein ◽  
Human Sera

ABSTRACT The E2 protein of hepatitis C virus (HCV) is believed to be a virion surface glycoprotein that is a candidate for inclusion in an antiviral vaccine. A truncated soluble version of E2 has recently been shown to interact with CD81, suggesting that this protein may be a component of the receptor for HCV. When expressed in eukaryotic cells, a significant proportion of E2 forms misfolded aggregates. To analyze the specificity of interaction between E2 and CD81, the aggregated and monomeric forms of a truncated E2 glycoprotein (E2661) were separated by high-pressure liquid chromatography and analyzed for CD81 binding. Nonaggregated forms of E2 preferentially bound CD81 and a number of conformation-dependent monoclonal antibodies (MAbs). Furthermore, intracellular forms of E2661 were found to bind CD81 with greater affinity than the extracellular forms. Intracellular and secreted forms of E2661 were also found to differ in reactivity with MAbs and human sera, consistent with differences in antigenicity. Together, these data indicate that proper folding of E2 is important for its interaction with CD81 and that modifications of glycans can modulate this interaction. Identification of the biologically active forms of E2 will assist in the future design of vaccines to protect against HCV infection.

Characterization of the nuclear localization signal and subcellular distribution of hepatitis C virus nonstructural protein NS5A

Gene ◽  
1996 ◽  
Vol 182 (1-2) ◽  
pp. 203-211 ◽  
Author(s):  
Yoshihiro Ide ◽  
Luwen Zhang ◽  
Min Chen ◽  
Genevieve Inchauspe ◽  
Chander Bahl ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Subcellular Distribution ◽  
Nonstructural Protein ◽  
Localization Signal
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