scholarly journals Energy Landscape of Alginate-Epimerase Interactions Assessed by Optical Tweezers and Atomic Force Microscopy

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141237 ◽  
Author(s):  
Armend Gazmeno Håti ◽  
Finn Lillelund Aachmann ◽  
Bjørn Torger Stokke ◽  
Gudmund Skjåk-Bræk ◽  
Marit Sletmoen
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Tweezers ◽  
Energy Landscape ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals DNA-Induced Viral Assembly Studied in Real-Time by Optical Tweezers, Acoustic Force Spectroscopy and Atomic Force Microscopy

2015 ◽  
Vol 108 (2) ◽  
pp. 170a
Author(s):  
Mariska G.M. van Rosmalen ◽  
Andreas S. Biebricher ◽  
Douwe Kamsma ◽  
Adam Zlotnick ◽  
Gijs J.L. Wuite ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Real Time ◽  
Optical Tweezers ◽  
Force Spectroscopy ◽  
Viral Assembly ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Oscillatory Microrheology, Creep Compliance and Stress Relaxation of Biological Cells Reveal Strong Correlations as Probed by Atomic Force Microscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
D.A.D. Flormann ◽  
C. Anton ◽  
M.O. Pohland ◽  
Y. Bautz ◽  
K. Kaub ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Stress Relaxation ◽  
Optical Tweezers ◽  
Power Law ◽  
Creep Compliance ◽  
Culture Dish ◽  
Experimental Conditions ◽  
Force Microscopy ◽  
Atomic Force

The mechanical properties of cells are important for many biological processes, including wound healing, cancers, and embryogenesis. Currently, our understanding of cell mechanical properties remains incomplete. Different techniques have been used to probe different aspects of the mechanical properties of cells, among them microplate rheology, optical tweezers, micropipette aspiration, and magnetic twisting cytometry. These techniques have given rise to different theoretical descriptions, reaching from simple Kelvin-Voigt or Maxwell models to fractional such as power law models, and their combinations. Atomic force microscopy (AFM) is a flexible technique that enables global and local probing of adherent cells. Here, using an AFM, we indented single retinal pigmented epithelium cells adhering to the bottom of a culture dish. The indentation was performed at two locations: above the nucleus, and towards the periphery of the cell. We applied creep compliance, stress relaxation, and oscillatory rheological tests to wild type and drug modified cells. Considering known fractional and semi-fractional descriptions, we found the extracted parameters to correlate. Moreover, the Young’s modulus as obtained from the initial indentation strongly correlated with all of the parameters from the applied power-law descriptions. Our study shows that the results from different rheological tests are directly comparable. This can be used in the future, for example, to reduce the number of measurements in planned experiments. Apparently, under these experimental conditions, the cells possess a limited number of degrees of freedom as their rheological properties change.

scholarly journals Detection of streptavidin–biotin intermediate metastable states at the single-molecule level using high temporal-resolution atomic force microscopy

RSC Advances ◽  
2019 ◽  
Vol 9 (39) ◽  
pp. 22705-22712 ◽  
Author(s):  
Evan Angelo Mondarte ◽  
Tatsuhiro Maekawa ◽  
Takashi Nyu ◽  
Hiroyuki Tahara ◽  
Ganchimeg Lkhamsuren ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Temporal Resolution ◽  
Single Molecule ◽  
Energy Landscape ◽  
Metastable States ◽  
High Temporal Resolution ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biotin Binding

Energy landscape illustration from the streptavidin–biotin binding dynamics observed in high temporal-resolution AFM.

Free Energy Landscape and Dynamics of Supercoiled DNA by High-Speed Atomic Force Microscopy

ACS Nano ◽  
2018 ◽  
Vol 12 (12) ◽  
pp. 11907-11916 ◽  
Author(s):  
Tine Brouns ◽  
Herlinde De Keersmaecker ◽  
Sebastian F. Konrad ◽  
Noriyuki Kodera ◽  
Toshio Ando ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Free Energy ◽  
High Speed ◽  
Energy Landscape ◽  
Free Energy Landscape ◽  
Supercoiled Dna ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy combined with optical tweezers (AFM/OT)

2016 ◽  
Vol 27 (2) ◽  
pp. 025904 ◽  
Author(s):  
F Pierini ◽  
K Zembrzycki ◽  
P Nakielski ◽  
S Pawłowska ◽  
T A Kowalewski
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Tweezers ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Bioconjugation Strategies for Connecting Proteins to DNA-Linkers for Single-Molecule Force-Based Experiments

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2424
Author(s):  
Lyan M. van der Sleen ◽  
Katarzyna M. Tych
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Optical Tweezers ◽  
Single Molecule ◽  
Short Range ◽  
Force Spectroscopy ◽  
Magnetic Tweezers ◽  
Single Molecule Force Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force

The mechanical properties of proteins can be studied with single molecule force spectroscopy (SMFS) using optical tweezers, atomic force microscopy and magnetic tweezers. It is common to utilize a flexible linker between the protein and trapped probe to exclude short-range interactions in SMFS experiments. One of the most prevalent linkers is DNA due to its well-defined properties, although attachment strategies between the DNA linker and protein or probe may vary. We will therefore provide a general overview of the currently existing non-covalent and covalent bioconjugation strategies to site-specifically conjugate DNA-linkers to the protein of interest. In the search for a standardized conjugation strategy, considerations include their mechanical properties in the context of SMFS, feasibility of site-directed labeling, labeling efficiency, and costs.

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