Forced Unbinding of Individual Urea–Aminotriazine Supramolecular Polymers by Atomic Force Microscopy: A Closer Look at the Potential Energy Landscape and Binding Lengths at Fixed Loading Rates

2011 ◽  
Vol 116 (1) ◽  
pp. 565-570 ◽  
Author(s):  
Anika Embrechts ◽  
Holger Schönherr ◽  
G. Julius Vancso
Keyword(s):  
Atomic Force Microscopy ◽  
Potential Energy ◽  
Energy Landscape ◽  
Supramolecular Polymers ◽  
Force Microscopy ◽  
Loading Rates ◽  
Atomic Force ◽  
Potential Energy Landscape

Rate dependence of serrated flow in a metallic glass

2003 ◽  
Vol 18 (4) ◽  
pp. 755-757 ◽  
Author(s):  
W. H. Jiang ◽  
M. Atzmon
Keyword(s):  
Plastic Deformation ◽  
Atomic Force Microscopy ◽  
Loading Rate ◽  
Shear Bands ◽  
Shear Band Formation ◽  
Band Formation ◽  
Serrated Flow ◽  
Force Microscopy ◽  
Loading Rates ◽  
Atomic Force

Plastic deformation of amorphous Al90Fe5Gd5 was investigated using nanoindentation and atomic force microscopy. While serrated flow was detected only at high loading rates, shear bands were observed for all loading rates, ranging from 1 to 100 nm/s. However, the details of shear-band formation depend on the loading rate.

scholarly journals Detection of streptavidin–biotin intermediate metastable states at the single-molecule level using high temporal-resolution atomic force microscopy

RSC Advances ◽  
2019 ◽  
Vol 9 (39) ◽  
pp. 22705-22712 ◽  
Author(s):  
Evan Angelo Mondarte ◽  
Tatsuhiro Maekawa ◽  
Takashi Nyu ◽  
Hiroyuki Tahara ◽  
Ganchimeg Lkhamsuren ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Temporal Resolution ◽  
Single Molecule ◽  
Energy Landscape ◽  
Metastable States ◽  
High Temporal Resolution ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biotin Binding

Energy landscape illustration from the streptavidin–biotin binding dynamics observed in high temporal-resolution AFM.

Free Energy Landscape and Dynamics of Supercoiled DNA by High-Speed Atomic Force Microscopy

ACS Nano ◽  
2018 ◽  
Vol 12 (12) ◽  
pp. 11907-11916 ◽  
Author(s):  
Tine Brouns ◽  
Herlinde De Keersmaecker ◽  
Sebastian F. Konrad ◽  
Noriyuki Kodera ◽  
Toshio Ando ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Free Energy ◽  
High Speed ◽  
Energy Landscape ◽  
Free Energy Landscape ◽  
Supercoiled Dna ◽  
Force Microscopy ◽  
Atomic Force

Energy Landscape of Streptavidin−Biotin Complexes Measured by Atomic Force Microscopy†

Biochemistry ◽  
2000 ◽  
Vol 39 (33) ◽  
pp. 10219-10223 ◽  
Author(s):  
Chunbo Yuan ◽  
Aileen Chen ◽  
Pamela Kolb ◽  
Vincent T. Moy
Keyword(s):  
Atomic Force Microscopy ◽  
Energy Landscape ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Energy Landscape of Alginate-Epimerase Interactions Assessed by Optical Tweezers and Atomic Force Microscopy

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141237 ◽  
Author(s):  
Armend Gazmeno Håti ◽  
Finn Lillelund Aachmann ◽  
Bjørn Torger Stokke ◽  
Gudmund Skjåk-Bræk ◽  
Marit Sletmoen
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Tweezers ◽  
Energy Landscape ◽  
Force Microscopy ◽  
Atomic Force

Rate- and depth-dependent nanomechanical behavior of individual living Chinese hamster ovary cells probed by atomic force microscopy

2006 ◽  
Vol 21 (8) ◽  
pp. 1906-1912 ◽  
Author(s):  
Minhua Zhao ◽  
Charudharshini Srinivasan ◽  
Diane J. Burgess ◽  
Bryan D. Huey
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Viscoelastic Behavior ◽  
Elastic Behavior ◽  
Force Microscopy ◽  
Loading Rates ◽  
Atomic Force

A single elastic modulus is not sufficient for describing the mechanical behavior of a living cell due to its viscoelastic nature and heterogeneity beneath the membrane. In this paper, the nanoscale elastic and viscoelastic behavior of individual living Chinese hamster ovary (CHO-K1) cells in a physiological environment were probed by atomic force microscopy (AFM) indentations at various loading rates. Based on Hertzian fits of the force–distance curves, the apparent elastic modulus of the cells was determined and found to be a function of the loading rate as well as the indentation depth. Notably, contributions from the substrate were negligible up to 50% of the cell thickness. For increased indentation rates and depths, healthy spindle-shaped CHO-K1 cells were found to exhibit an increased change of stiffness, whereas for unhealthy oval- shaped CHO-K1 cells there was little stiffening at equivalent loading rates and depths. Furthermore, a larger hysteresis between the loading and unloading curves was observed with increasing loading rates, which was related to the viscoelastic behavior of CHO-K1 cells. This work demonstrates differences in the rate- and depth-dependent elastic behavior at the nanoscale level between healthy and unhealthy mammalian cells.

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