scholarly journals Aberrant Expression of Interleukin-1β and Inflammasome Activation in Human Malignant Gliomas

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e103432 ◽  
Author(s):  
Leonid Tarassishin ◽  
Diana Casper ◽  
Sunhee C. Lee
Keyword(s):  
Malignant Gliomas ◽  
Interleukin 1Β ◽  
Inflammasome Activation ◽  
Aberrant Expression

scholarly journals Aberrant Expression of N-Methylpurine-DNA Glycosylase Influences Patient Survival in Malignant Gliomas

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Ce Liu ◽  
Yanyang Tu ◽  
Jun Yuan ◽  
Xinggang Mao ◽  
Shiming He ◽  
...  
Keyword(s):  
Patient Survival ◽  
Malignant Gliomas ◽  
Dna Glycosylase ◽  
Aberrant Expression ◽  
Methylpurine Dna Glycosylase

scholarly journals Hepatic inflammasome activation as origin of Interleukin-1α and Interleukin-1β in liver cirrhosis

Gut ◽  
2020 ◽  
pp. gutjnl-2020-322621
Author(s):  
Michael Praktiknjo ◽  
Robert Schierwagen ◽  
Sofia Monteiro ◽  
Cristina Ortiz ◽  
Frank Erhard Uschner ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Interleukin 1Β ◽  
Inflammasome Activation ◽  
Interleukin 1Α

Comparison of Interleukin-1β Expression by In Situ Hybridization in Monoclonal Gammopathy of Undetermined Significance and Multiple Myeloma

Blood ◽  
1999 ◽  
Vol 93 (1) ◽  
pp. 300-305 ◽  
Author(s):  
Martha Q. Lacy ◽  
Kathleen A. Donovan ◽  
Julie K. Heimbach ◽  
Gregory J. Ahmann ◽  
John A. Lust
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Monoclonal Gammopathy ◽  
Plasma Cells ◽  
Interleukin 1Β ◽  
Clinical Feature ◽  
Bone Lesions ◽  
Aberrant Expression ◽  
Undetermined Significance

Abstract We investigated whether interleukin-1β (IL-1β) is differentially expressed in plasma cells from monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) patients because IL-1β appears to play a major role in the development of lytic bone lesions, the major clinical feature distinguishing MGUS from myeloma. In situ hybridization (ISH) for IL-1β was performed using bone marrow aspirates from 51 MM, 7 smoldering MM, 21 MGUS, and 5 normal control samples. Using the ISH technique IL-1β mRNA was detectable in the plasma cells from 49 of 51 patients with active myeloma and 7 of 7 patients with smoldering myeloma. In contrast, 5 of 21 patients with MGUS and 0 of 5 normal controls had detectable IL-1β message. Bone lesions were present in 40 of the 51 MM patients analyzed, and all 40 patients had IL-1β mRNA by ISH. These results show that greater than 95% of MM patients but less than 25% of MGUS patients are positive for IL-1β production. In the future, continued follow-up of IL-1β positive and negative MGUS patients should determine whether aberrant expression of plasma cell IL-1β is predictive of those MGUS patients that will eventually progress to active myeloma.

Aberrant expression of the sox2 gene in malignant gliomas

2014 ◽  
Vol 8 (5) ◽  
pp. 368-373 ◽  
Author(s):  
A. V. Volnitskiy ◽  
E. V. Semenova ◽  
T. A. Shtam ◽  
R. A. Kovalev ◽  
M. V. Filatov
Keyword(s):  
Malignant Gliomas ◽  
Aberrant Expression ◽  
Sox2 Gene

scholarly journals Candida albicans Morphogenesis Is Not Required for Macrophage Interleukin 1β Production

mBio ◽  
2012 ◽  
Vol 4 (1) ◽  
Author(s):  
Melanie Wellington ◽  
Kristy Koselny ◽  
Damian J. Krysan
Keyword(s):  
Transcription Factor ◽  
Candida Albicans ◽  
Interleukin 1Β ◽  
Inflammasome Activation ◽  
Wild Type ◽  
Content Type ◽  
Hypha Formation ◽  
Physical Presence ◽  
J774 Cells ◽  
Low Levels

ABSTRACTThe interaction ofCandida albicanswith macrophages induces the production of interleukin 1β (IL-1β) through inflammasome activation in a process that is required for host survival.C. albicanshypha formation has been linked to IL-1β production, but the question of whether hyphae are sufficient to trigger IL-1β production has not been examined directly. To address this question, aC. albicanslibrary of 165 transcription factor deletion mutants was screened for strains with altered IL-1β production by lipopolysaccharide (LPS)-primed J774 cells, a murine macrophage-like cell line. Eight mutants with decreased and two mutants with increased IL-1β secretion were identified. In addition, 12 mutants with previously identified morphology deficits were found to induce IL-1β secretion to levels similar to those of the wild type. Examination of the morphology of both low and normal IL-1β-inducing mutants in macrophages revealed that two mutants (upc2Δ/upc2Δ andahr1Δ/Δ mutants) were indistinguishable from the wild type with respect to morphology yet induced low levels of IL-1β; conversely, thendt80Δ/Δ mutant was deficient for hypha formation but induced levels of IL-1β similar to those of the wild type. Transcription factor mutants deficient for IL-1β secretion also caused markedly lower levels of macrophage lysis. Similarly, the ability of a mutant to cause macrophage lysis was independent of its ability to form hyphae. Taken together, our observations indicate that the physical formation of hyphae is not sufficient to trigger IL-1β secretion or macrophage lysis and suggest that other mechanisms, such as pyroptosis, a caspase-1-dependent response to intracellular pathogens, may play a role in the interaction of macrophages withC. albicans.IMPORTANCEThe ability ofCandida albicansto transition from yeast to filamentous cells plays an important and complex role in pathogenesis. Recent results from a number of investigators indicate that the host responds to yeast and hyphalC. albicansdifferently. For example, aC. albicansmutant unable to form hyphae also fails to induce interleukin 1β (IL-1β) secretion from macrophages. We have identifiedC. albicanstranscription factor mutants that have decreased IL-1β secretion but retain the ability to form hyphae in response to macrophages. In addition, these mutants cause significantly less macrophage lysis. These observations indicate that the physical presence of the hyphal structure in the macrophage is not sufficient to trigger IL-1β secretion nor does it cause physical lysis of the cell. Our data indicate that characteristics of hyphae separate from its physical morphology are responsible for triggering the release of IL-1β release and causing macrophage lysis. Since these observations are inconsistent with some current models, alternative mechanisms for the interaction ofC. albicanswith macrophages must be considered.

scholarly journals Bypassing Pathogen‐Induced Inflammasome Activation for the Regulation of Interleukin‐1β Production by the Fungal PathogenCandida albicans

2009 ◽  
Vol 199 (7) ◽  
pp. 1087-1096 ◽  
Author(s):  
Frank L. van de Veerdonk ◽  
Leo A. B. Joosten ◽  
Isabel Devesa ◽  
Héctor M. Mora‐Montes ◽  
Thirumala‐Devi Kanneganti ◽  
...  
Keyword(s):  
Interleukin 1Β ◽  
Inflammasome Activation

scholarly journals Implication of the NLRP3 Inflammasome in Bovine Age-Related Sarcopenia

2021 ◽  
Vol 22 (7) ◽  
pp. 3609
Author(s):  
Davide De Biase ◽  
Giuseppe Piegari ◽  
Francesco Prisco ◽  
Ilaria Cimmino ◽  
Ilaria d’Aquino ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Nlrp3 Inflammasome ◽  
Interleukin 1Β ◽  
Inflammasome Activation ◽  
Interleukin 18 ◽  
Necrosis Factor Alpha ◽  
Age Related ◽  
Factor Alpha ◽  
Bovine Skeletal Muscle ◽  
First Time

Sarcopenia is defined as the age-related loss of skeletal muscle mass, quality, and strength. The pathophysiological mechanisms underlying sarcopenia are still not completely understood. The aim of this work was to evaluate, for the first time, the expression of NLRP3 inflammasome in bovine skeletal muscle in order to investigate the hypothesis that inflammasome activation may trigger and sustain a pro-inflammatory environment leading to sarcopenia. Samples of skeletal muscle were collected from 60 cattle belonging to three age-based groups. Morphologic, immunohistochemical and molecular analysis were performed to assess the presence of age-related pathologic changes and chronic inflammation, the expression of NLRP3 inflammasome and to determine the levels of interleukin-1β, interleukin-18 and tumor necrosis factor alpha in muscle tissue. Our results revealed the presence of morphologic sarcopenia hallmark, chronic lymphocytic inflammation and a type II fibers-selective NLRP3 expression associated to a significant decreased number of immunolabeled-fibers in aged animals. Moreover, we found a statistically significant age-related increase of pro-inflammatory cytokines such as interleukin-1β and interleukin-18 suggesting the activation of NLRP3 inflammasome. Taken together, our data suggest that NLRP3 inflammasome components may be normally expressed in skeletal muscle, but its priming and activation during aging may contribute to enhance a pro-inflammatory environment altering normal muscular anabolism and metabolism.

Comparison of Interleukin-1β Expression by In Situ Hybridization in Monoclonal Gammopathy of Undetermined Significance and Multiple Myeloma

Blood ◽  
1999 ◽  
Vol 93 (1) ◽  
pp. 300-305 ◽  
Author(s):  
Martha Q. Lacy ◽  
Kathleen A. Donovan ◽  
Julie K. Heimbach ◽  
Gregory J. Ahmann ◽  
John A. Lust
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Monoclonal Gammopathy ◽  
Plasma Cells ◽  
Interleukin 1Β ◽  
Clinical Feature ◽  
Bone Lesions ◽  
Aberrant Expression ◽  
Undetermined Significance

We investigated whether interleukin-1β (IL-1β) is differentially expressed in plasma cells from monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) patients because IL-1β appears to play a major role in the development of lytic bone lesions, the major clinical feature distinguishing MGUS from myeloma. In situ hybridization (ISH) for IL-1β was performed using bone marrow aspirates from 51 MM, 7 smoldering MM, 21 MGUS, and 5 normal control samples. Using the ISH technique IL-1β mRNA was detectable in the plasma cells from 49 of 51 patients with active myeloma and 7 of 7 patients with smoldering myeloma. In contrast, 5 of 21 patients with MGUS and 0 of 5 normal controls had detectable IL-1β message. Bone lesions were present in 40 of the 51 MM patients analyzed, and all 40 patients had IL-1β mRNA by ISH. These results show that greater than 95% of MM patients but less than 25% of MGUS patients are positive for IL-1β production. In the future, continued follow-up of IL-1β positive and negative MGUS patients should determine whether aberrant expression of plasma cell IL-1β is predictive of those MGUS patients that will eventually progress to active myeloma.

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