scholarly journals Transcriptomic Analysis Reveals Novel Mechanistic Insight into Murine Biological Responses to Multi-Walled Carbon Nanotubes in Lungs and Cultured Lung Epithelial Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e80452 ◽  
Author(s):  
Sarah Søs Poulsen ◽  
Nicklas R. Jacobsen ◽  
Sarah Labib ◽  
Dongmei Wu ◽  
Mainul Husain ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Transcriptomic Analysis ◽  
Lung Epithelial Cells ◽  
Mechanistic Insight ◽  
Biological Responses ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Insight Into

Toxicological assessment of multi-walled carbon nanotubes on A549 human lung epithelial cells

2015 ◽  
Vol 29 (2) ◽  
pp. 352-362 ◽  
Author(s):  
Giuseppa Visalli ◽  
Maria Paola Bertuccio ◽  
Daniela Iannazzo ◽  
Anna Piperno ◽  
Alessandro Pistone ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Toxicological Assessment ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Human Lung Epithelial Cells

Multi-walled carbon nanotubes induce cytotoxicity and genotoxicity in human lung epithelial cells

2012 ◽  
Vol 32 (6) ◽  
pp. 454-464 ◽  
Author(s):  
Delia Cavallo ◽  
Carla Fanizza ◽  
Cinzia Lucia Ursini ◽  
Stefano Casciardi ◽  
Emilia Paba ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Human Lung Epithelial Cells

Dispersion, Characterization and Uptake of Multi-Walled Carbon Nanotubes by Epithelial Cells

2015 ◽  
Vol 34 ◽  
pp. 41-47 ◽  
Author(s):  
Shruti Sinha ◽  
Karshak Kosaraju ◽  
Shyam Aravamudhan
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Biomedical Applications ◽  
Neural Stimulation ◽  
Lung Epithelial Cells ◽  
Know How ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
A549 Lung

Multi-walled Carbon Nanotubes (MWCNTs) have a great potential for microelectronics, optics and in biomedical applications (e.g. as nanoelectrodes for neural stimulation and functional scaffolds for tissue engineering). In order to understand the interactions of MWCNTs with the human body on a whole, it is important to know how they interact with the cells. In this study, we dispersed the MWCNTs with anionic surfactant, characterized the properties of dispersed MWCNTs, and then evaluated the uptake of MWCNTs by A549 lung epithelial cells using Confocal Raman spectroscopy.

scholarly journals Mitsui-7, heat-treated, and nitrogen-doped multi-walled carbon nanotubes elicit genotoxicity in human lung epithelial cells

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Katelyn J. Siegrist ◽  
Steven H. Reynolds ◽  
Dale W. Porter ◽  
Robert R. Mercer ◽  
Alison K. Bauer ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Human Lung ◽  
S Phase ◽  
Lung Epithelial Cells ◽  
International Agency ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Human Lung Epithelial Cells

Abstract Background The unique physicochemical properties of multi-walled carbon nanotubes (MWCNT) have led to many industrial applications. Due to their low density and small size, MWCNT are easily aerosolized in the workplace making respiratory exposures likely in workers. The International Agency for Research on Cancer designated the pristine Mitsui-7 MWCNT (MWCNT-7) as a Group 2B carcinogen, but there was insufficient data to classify all other MWCNT. Previously, MWCNT exposed to high temperature (MWCNT-HT) or synthesized with nitrogen (MWCNT-ND) have been found to elicit attenuated toxicity; however, their genotoxic and carcinogenic potential are not known. Our aim was to measure the genotoxicity of MWCNT-7 compared to these two physicochemically-altered MWCNTs in human lung epithelial cells (BEAS-2B & SAEC). Results Dose-dependent partitioning of individual nanotubes in the cell nuclei was observed for each MWCNT material and was greatest for MWCNT-7. Exposure to each MWCNT led to significantly increased mitotic aberrations with multi- and monopolar spindle morphologies and fragmented centrosomes. Quantitative analysis of the spindle pole demonstrated significantly increased centrosome fragmentation from 0.024–2.4 μg/mL of each MWCNT. Significant aneuploidy was measured in a dose-response from each MWCNT-7, HT, and ND; the highest dose of 24 μg/mL produced 67, 61, and 55%, respectively. Chromosome analysis demonstrated significantly increased centromere fragmentation and translocations from each MWCNT at each dose. Following 24 h of exposure to MWCNT-7, ND and/or HT in BEAS-2B a significant arrest in the G1/S phase in the cell cycle occurred, whereas the MWCNT-ND also induced a G2 arrest. Primary SAEC exposed for 24 h to each MWCNT elicited a significantly greater arrest in the G1 and G2 phases. However, SAEC arrested in the G1/S phase after 72 h of exposure. Lastly, a significant increase in clonal growth was observed one month after exposure to 0.024 μg/mL MWCNT-HT & ND. Conclusions Although MWCNT-HT & ND cause a lower incidence of genotoxicity, all three MWCNTs cause the same type of mitotic and chromosomal disruptions. Chromosomal fragmentation and translocations have not been observed with other nanomaterials. Because in vitro genotoxicity is correlated with in vivo genotoxic response, these studies in primary human lung cells may predict the genotoxic potency in exposed human populations.

scholarly journals Multi-Walled Carbon Nanotubes (MWCNTs) Cause Cellular Senescence in TGF-β Stimulated Lung Epithelial Cells

Toxics ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 144
Author(s):  
Joseph H. Lucas ◽  
Qixin Wang ◽  
Thivanka Muthumalage ◽  
Irfan Rahman
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Cellular Senescence ◽  
Transforming Growth Factor ◽  
Plasminogen Activator Inhibitor ◽  
Transforming Growth Factor Β ◽  
Lung Epithelial Cells ◽  
Chronic Lung Diseases ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Multi-walled carbon nanotubes are engineered nanomaterials (ENMs) that have a fiber-like structure which may be a concern for the development of cellular senescence. Premature senescence, a state of irreversible cell cycle arrest, is implicated in the pathogenesis of chronic lung diseases such as pulmonary fibrosis (PF). However, the crosstalk between downstream pathways mediating fibrotic and senescent responses of MWCNTs is not well-defined. Here, we exposed human bronchial epithelial cells (BEAS-2B) to MWCNTs for up to 72 h and demonstrate that MWCNTs increase reactive oxygen species (ROS) production accompanied by inhibition of cell proliferation. In addition, MWCNT exposure resulted in the increase of p21 protein abundance and senescence associated β-galactosidase (SA β-gal) activity. We also determined that co-exposure with the cytokine, transforming growth factor-β (TGF-β) exacerbated cellular senescence indicated by increased protein levels of p21, p16, and γH2A.X. Furthermore, the production of fibronectin and plasminogen activator inhibitor (PAI-1) was significantly elevated with the co-exposure compared to MWCNT or TGF-β alone. Together, our study suggests that the cellular senescence potential of MWCNTs may be enhanced by pro-fibrotic mediators, such as TGF-β in the surrounding microenvironment.

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