Multi-walled carbon nanotubes induce cytotoxicity and genotoxicity in human lung epithelial cells

2012 ◽  
Vol 32 (6) ◽  
pp. 454-464 ◽  
Author(s):  
Delia Cavallo ◽  
Carla Fanizza ◽  
Cinzia Lucia Ursini ◽  
Stefano Casciardi ◽  
Emilia Paba ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Human Lung Epithelial Cells

Toxicological assessment of multi-walled carbon nanotubes on A549 human lung epithelial cells

2015 ◽  
Vol 29 (2) ◽  
pp. 352-362 ◽  
Author(s):  
Giuseppa Visalli ◽  
Maria Paola Bertuccio ◽  
Daniela Iannazzo ◽  
Anna Piperno ◽  
Alessandro Pistone ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Toxicological Assessment ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Human Lung Epithelial Cells

scholarly journals Mitsui-7, heat-treated, and nitrogen-doped multi-walled carbon nanotubes elicit genotoxicity in human lung epithelial cells

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Katelyn J. Siegrist ◽  
Steven H. Reynolds ◽  
Dale W. Porter ◽  
Robert R. Mercer ◽  
Alison K. Bauer ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Human Lung ◽  
S Phase ◽  
Lung Epithelial Cells ◽  
International Agency ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Human Lung Epithelial Cells

Abstract Background The unique physicochemical properties of multi-walled carbon nanotubes (MWCNT) have led to many industrial applications. Due to their low density and small size, MWCNT are easily aerosolized in the workplace making respiratory exposures likely in workers. The International Agency for Research on Cancer designated the pristine Mitsui-7 MWCNT (MWCNT-7) as a Group 2B carcinogen, but there was insufficient data to classify all other MWCNT. Previously, MWCNT exposed to high temperature (MWCNT-HT) or synthesized with nitrogen (MWCNT-ND) have been found to elicit attenuated toxicity; however, their genotoxic and carcinogenic potential are not known. Our aim was to measure the genotoxicity of MWCNT-7 compared to these two physicochemically-altered MWCNTs in human lung epithelial cells (BEAS-2B & SAEC). Results Dose-dependent partitioning of individual nanotubes in the cell nuclei was observed for each MWCNT material and was greatest for MWCNT-7. Exposure to each MWCNT led to significantly increased mitotic aberrations with multi- and monopolar spindle morphologies and fragmented centrosomes. Quantitative analysis of the spindle pole demonstrated significantly increased centrosome fragmentation from 0.024–2.4 μg/mL of each MWCNT. Significant aneuploidy was measured in a dose-response from each MWCNT-7, HT, and ND; the highest dose of 24 μg/mL produced 67, 61, and 55%, respectively. Chromosome analysis demonstrated significantly increased centromere fragmentation and translocations from each MWCNT at each dose. Following 24 h of exposure to MWCNT-7, ND and/or HT in BEAS-2B a significant arrest in the G1/S phase in the cell cycle occurred, whereas the MWCNT-ND also induced a G2 arrest. Primary SAEC exposed for 24 h to each MWCNT elicited a significantly greater arrest in the G1 and G2 phases. However, SAEC arrested in the G1/S phase after 72 h of exposure. Lastly, a significant increase in clonal growth was observed one month after exposure to 0.024 μg/mL MWCNT-HT & ND. Conclusions Although MWCNT-HT & ND cause a lower incidence of genotoxicity, all three MWCNTs cause the same type of mitotic and chromosomal disruptions. Chromosomal fragmentation and translocations have not been observed with other nanomaterials. Because in vitro genotoxicity is correlated with in vivo genotoxic response, these studies in primary human lung cells may predict the genotoxic potency in exposed human populations.

scholarly journals Carbon Nanotubes Induce Apoptosis Resistance of Human Lung Epithelial Cells Through FLICE-Inhibitory Protein

2014 ◽  
Vol 143 (2) ◽  
pp. 499-511 ◽  
Author(s):  
Varisa Pongrakhananon ◽  
Sudjit Luanpitpong ◽  
Todd A. Stueckle ◽  
Liying Wang ◽  
Ubonthip Nimmannit ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Apoptosis Resistance ◽  
Inhibitory Protein ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

Comparative cyto-genotoxicity assessment of functionalized and pristine multiwalled carbon nanotubes on human lung epithelial cells

2012 ◽  
Vol 26 (6) ◽  
pp. 831-840 ◽  
Author(s):  
Cinzia Lucia Ursini ◽  
Delia Cavallo ◽  
Anna Maria Fresegna ◽  
Aureliano Ciervo ◽  
Raffaele Maiello ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Epithelial Cells ◽  
Multiwalled Carbon Nanotubes ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Multiwalled Carbon ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells
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