scholarly journals Temporal Characterization of Homology-Independent Centromere Coupling in Meiotic Prophase

PLoS ONE ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. e10336 ◽  
Author(s):  
David Obeso ◽  
Dean S. Dawson
Keyword(s):  
Meiotic Prophase

scholarly journals Characterization of cDNAs Induced in Meiotic Prophase in Lily Microsporocytes

DNA Research ◽  
1994 ◽  
Vol 1 (1) ◽  
pp. 15-26 ◽  
Author(s):  
T. Kobayashi ◽  
E. Kobayashi ◽  
S. Sato ◽  
Y. Hotta ◽  
N. Miyajima ◽  
...  
Keyword(s):  
Meiotic Prophase

scholarly journals Characterization of Spo11-dependent and independent phospho-H2AX foci during meiotic prophase I in the male mouse

2007 ◽  
Vol 120 (10) ◽  
pp. 1733-1742 ◽  
Author(s):  
A. Chicheportiche ◽  
J. Bernardino-Sgherri ◽  
B. de Massy ◽  
B. Dutrillaux
Keyword(s):  
Meiotic Prophase ◽  
Male Mouse ◽  
Prophase I ◽  
Meiotic Prophase I

Chromosomal synapsis and the meiotic process in male mesquite lizards, Sceloporus grammicus complex

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 398-408 ◽  
Author(s):  
Kent M. Reed ◽  
Jack W. Sites Jr. ◽  
Ira F. Greenbaum
Keyword(s):  
Synaptonemal Complex ◽  
Sex Chromosomes ◽  
Meiotic Prophase ◽  
Morphological Characterization ◽  
Autosomal Bivalents ◽  
Meiotic Process ◽  
Low Levels ◽  
Sceloporus Grammicus ◽  
Metaphase I

Meiosis in males of the F5 cytotype of Sceloporus grammicus was examined through the analysis of synaptonemal complexes (SCs), diakinetic (metaphase I) nuclei, and secondary spermatocytes (metaphase II configurations). These data allowed the establishment of criteria for substaging of zygonema and pachynema, morphological characterization of the SC complement, and comparison of the orientation and segregation of the autosomes and sex chromosomes. The analysis of nuclei from all stages of meiotic prophase I (leptonema through diakinesis) provided a useful means of partitioning the temporal sequence of early meiotic events. Three substages of zygonema (Z1–Z3) were established, based on the extent of synapsis of the microchromosomal and macrochromosomal elements. Synaptic initiation of the autosomes and sex chromosomes was synchronous. Two patterns of macrochromosomal synapsis were observed. Whereas synapsis of the biarmed elements was biterminal (i.e., progressing from both ends of the homologs), synapsis of the acrocentric elements was uniterminal involving only the distal (noncentromeric) ends of the homologs. Unique sex-chromosomal characteristics were not observed in S. grammicus and, therefore, the substaging of pachynema was based on subjective criteria. Examination of diakinesis – metaphase I and metaphase II configurations indicated low levels of diakinetic irregularities with balanced segregation of the autosomal bivalents and the sex-chromosomal trivalent.Key words: Sceloporus grammicus, meiosis, synaptonemal complex, substaging.

Characterization of fission yeast meiotic mutants based on live observation of meiotic prophase nuclear movement

Chromosoma ◽  
2000 ◽  
Vol 109 (1-2) ◽  
pp. 103-109 ◽  
Author(s):  
Yasushi Hiraoka ◽  
Da-Qiao Ding ◽  
Ayumu Yamamoto ◽  
Chihiro Tsutsumi ◽  
Yuji Chikashige
Keyword(s):  
Fission Yeast ◽  
Meiotic Prophase ◽  
Nuclear Movement ◽  
Meiotic Mutants

scholarly journals A General Method for Identifying Recessive Diploid-Specific Mutations in Saccharomyces cerevisiae, Its Application to the Isolation of Mutants Blocked at Intermediate Stages of Meiotic Prophase and Characterization of a New Gene SAE2

Genetics ◽  
1997 ◽  
Vol 146 (3) ◽  
pp. 797-816 ◽  
Author(s):  
Andrew H Z McKee ◽  
Nancy Kleckner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Prophase ◽  
Temperature Sensitive ◽  
Yeast Saccharomyces Cerevisiae ◽  
New Approach ◽  
Strand Breaks ◽  
Recessive Mutations ◽  
New Genes ◽  
New Gene

We describe a general new approach for identifying recessive mutations that affect diploid strains of yeast Saccharomyces cerevisiae and the application of this method to the identification of mutations that confer an intermediate block in meiotic prophase chromosome metabolism. The method uses a temperature-sensitive conjugation mutation ste7-1 in combination with homothallism. The mutations of interest confer a defect in spore formation that is dependent upon a gene required for initiation of meiotic recombination and development of meiosis-specific chromosome structure (SPO11). Identified in this screen were null mutations of the DMC1 gene, nonnull mutations of RAD50 (rad50S, and mutations in three new genes designated SAE1, SAE2 and SAE3 (Sporulation in the Absence of Spo Eleven). Molecular characterization of the SAE2 gene and characterization of meiotic and mitotic phenotypes of sae2 mutants are also presented. The phenotypes conferred by a sae2 null mutation are virtually indistinguishable from those conferred by the previously identified nonnull mutations of RAD50 (rad50S). Most notably, both mutations confer only weak sensitivity to the radiomimetic agent methyl methane sulfonate (MMS) but completely block resection and turnover of meiosis-specific double-strand breaks. These observations provide further evidence that this constellation of phenotypes identifies a specific molecular function.

Characterization of the diffuse stage in the male meiotic prophase and karyotype of Scapteriscus borellii (Orthoptera: Grylloidea)

Genetica ◽  
1986 ◽  
Vol 71 (1) ◽  
pp. 23-29 ◽  
Author(s):  
H. Cardoso ◽  
M. Stoll ◽  
A. Dutra ◽  
G. Oliver ◽  
M. V. Di Tomaso
Keyword(s):  
Meiotic Prophase ◽  
Diffuse Stage

Characterization of expressed meiotic prophase repeat transcript clones of Lilium: meiosis-specific expression, relatedness, and affinities to small heat shock protein genes

Genome ◽  
1990 ◽  
Vol 33 (1) ◽  
pp. 68-79 ◽  
Author(s):  
Robert A. Bouchard
Keyword(s):  
Heat Shock ◽  
Meiotic Prophase ◽  
Higher Plants ◽  
Small Heat Shock Protein ◽  
Heat Shock Gene ◽  
Cdna Clones ◽  
Restriction Maps ◽  
Specific Expression ◽  
Shock Gene

The inserts of plasmid cDNA clones for transcripts showing meiotic prophase specific expression show cross reassociation to varying degrees of intensity with one another. These clones were recovered from a cDNA library made from Lilium microsporocyte poly(A)+ RNA. RNA-dot and Northern-blot analyses indicate that these clones represent transcripts specific to the meiotic prophase interval in microsporocytes. The transcripts appear to constitute the most abundant class of meiosis-specific poly(A)+ RNAs. At least two subgroups can be distinguished by examining cloned transcripts from genes of this expressed meiotic prophase repeat (EMPR) sequence family. Members of each subgroup have similar although not identical restriction maps and show relatively high but varying fidelities of DNA cross reassociation between members. However, consensus restriction maps of the two subgroups are largely dissimilar and, except at low stringencies, cross reassociation is readily detected only at restriction fragments from a particular conserved internal segment. The DNA sequence of a representative EMPR clone has been determined, and the inferred peptide product has been found to show extensive sequence homology to that of a small heat-shock gene of Glycine max, particularly in the conserved region. Alignment of the sequences for the conserved regions of two EMPR subgroup representatives with the soybean sequence suggests that selection has acted to conserve similar blocks of amino acids in this area. These observations suggest that a major portion of the transcripts produced during the apparently unrelated processes of meiosis and heat shock in higher plants are derived from related gene sequences encoding similar products.Key words: meiosis, transcription, specific cDNA, heat-shock mRNA.

scholarly journals Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants

2011 ◽  
Vol 240 (7) ◽  
pp. 1779-1792 ◽  
Author(s):  
Kenji Saito ◽  
Kellee R. Siegfried ◽  
Christiane Nüsslein-Volhard ◽  
Noriyoshi Sakai
Keyword(s):  
Meiotic Prophase ◽  
Prophase I ◽  
Meiotic Prophase I ◽  
Cytogenetic Characterization
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