scholarly journals Cytoskeleton polarity is essential in determining orientational order in basal bodies of multi-ciliated cells

2020 ◽  
Vol 16 (2) ◽  
pp. e1007649
Author(s):  
Toshinori Namba ◽  
Shuji Ishihara
Keyword(s):  
Orientational Order ◽  
Basal Bodies ◽  
Ciliated Cells

Ciliary coordination in newt lung cells requires microtubule-based linkages between basal bodies: A correlative light and EM study

1992 ◽  
Vol 50 (1) ◽  
pp. 570-571
Author(s):  
Robert Hard ◽  
Gerald Rupp ◽  
Matthew L. Withiam-Leitch ◽  
Lisa Cardamone
Keyword(s):  
Basal Body ◽  
Untreated Control ◽  
Beat Frequency ◽  
Primary Cultures ◽  
Living Cells ◽  
Power Stroke ◽  
Ultrastructural Changes ◽  
Lung Cells ◽  
Basal Bodies ◽  
Ciliated Cells

In a coordinated field of beating cilia, the direction of the power stroke is correlated with the orientation of basal body appendages, called basal feet. In newt lung ciliated cells, adjacent basal feet are interconnected by cold-stable microtubules (basal MTs). In the present study, we investigate the hypothesis that these basal MTs stabilize ciliary distribution and alignment. To accomplish this, newt lung primary cultures were treated with the microtubule disrupting agent, Colcemid. In newt lung cultures, cilia normally disperse in a characteristic fashion as the mucociliary epithelium migrates from the tissue explant. Four arbitrary, but progressive stages of dispersion were defined and used to monitor this redistribution process. Ciliaiy beat frequency, coordination, and dispersion were assessed for 91 hrs in untreated (control) and treated cultures. When compared to controls, cilia dispersed more rapidly and ciliary coordination decreased markedly in cultures treated with Colcemid (2 mM). Correlative LM/EM was used to assess whether these effects of Colcemid were coupled to ultrastructural changes. Living cells were defined as having coordinated or uncoordinated cilia and then were processed for transmission EM.

Role of f-box factor foxj1 in differentiation of ciliated airway epithelial cells

2004 ◽  
Vol 286 (4) ◽  
pp. L650-L657 ◽  
Author(s):  
Yingjian You ◽  
Tao Huang ◽  
Edward J. Richer ◽  
Jens-Erik Harboe Schmidt ◽  
Joseph Zabner ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Basal Body ◽  
Ciliated Cell ◽  
Airway Epithelial Cells ◽  
Cell Phenotype ◽  
Basal Bodies ◽  
Airway Epithelial ◽  
Ciliated Cells ◽  
Wild Type

Factors required for commitment of an undifferentiated airway epithelial cell to a ciliated cell are unknown. Cell ultrastructure analysis indicates ciliated cell commitment activates a multistage program involving synthesis of cilia precursor proteins and assembly of macromolecular complexes. Foxj1 is an f-box transcription factor expressed in ciliated cells and shown to be required for cilia formation by gene deletion in a mouse model. To identify a specific role for foxj1 in directing the ciliated cell phenotype, we evaluated the capacity of foxj1 to induce ciliogenesis and direct cilia assembly. In a primary culture model of wild-type mouse airway epithelial cells, foxj1 expression preceded the appearance of cilia and in cultured foxj1 null cells cilia did not develop. Delivery of foxj1 to polarized epithelial cell lines and primary cultured alveolar epithelial cells failed to promote ciliogenesis. Similarly, delivery of foxj1 to wild-type airway epithelial cells did not enhance the total number of ciliated cells. In contrast, delivery of foxj1 to null cells resulted in the appearance of cilia. Analysis revealed that, in the absence of foxj1, null cells contained cilia precursor basal bodies, indicating prior commitment to ciliogenesis. However, the basal bodies were disorganized within the apical compartment and failed to dock with the apical membrane. Reconstitution of foxj1 in null cells restored normal basal body organization, resulting in axoneme growth. Thus foxj1 functions in late-stage ciliogenesis to regulate programs promoting basal body docking and axoneme formation in cells previously committed to the ciliated cell phenotype.

MOTION ACTIVITY OF AIRWAY CILIATED EPITHELIUM IN PATIENTS WITH ASTHMA

2016 ◽  
Vol 1 (62) ◽  
pp. 40-46
Author(s):  
Михаил Луценко ◽  
Mikhail Lutsenko
Keyword(s):  
Inflammatory Process ◽  
Mucociliary Clearance ◽  
Severe Form ◽  
Basal Bodies ◽  
Ciliated Epithelium ◽  
Epithelial Layer ◽  
Bronchial Mucosa ◽  
Ciliated Cells ◽  
Motion Activity ◽  
Mucociliary System

At asthma in bronchial mucous tunic as a result of chronic inflammatory process the working of mucociliary system is disturbed and there is restructuring of epithelial layer. The number of ciliary cells at the severe form of asthma decreases till 70%. The reason of restructuring of ciliated epithelium is the accumulation of a big number of fatty acids peroxides in the mucous tunic. Under their influence there is a decrease of activity of succinate dehydrogenase and ATP in the basal bodies of ciliary cells. Under the severe form of asthma there is a suppression of activity of mucociliary clearance as a result of destruction of a big number of bronchial mucosa ciliated cells.

scholarly journals Myosin at the apical pole of ciliated epithelial cells as revealed by a monoclonal antibody.

1986 ◽  
Vol 103 (2) ◽  
pp. 613-619 ◽  
Author(s):  
C Klotz ◽  
N Bordes ◽  
M C Laine ◽  
D Sandoz ◽  
M Bornens
Keyword(s):  
Monoclonal Antibody ◽  
Striated Muscle ◽  
Ultrastructural Localization ◽  
Basal Bodies ◽  
Myosin Heavy Chains ◽  
Ciliated Cells ◽  
Western Blots ◽  
Apical Pole ◽  
Heavy Chains ◽  
Filamentous Material

A monoclonal antibody (CC-212), obtained in a fusion experiment in which basal bodies from quail oviduct were used as immunogen, has been shown to label the apical pole of ciliated cells and to react with a 200-kD protein. This monoclonal antibody was demonstrated to be an anti-myosin from smooth muscle or from nonmuscular cells using the following criteria: On Western blots it reacted with the myosin heavy chains from gizzard and platelet extracts and from cultured cell line extracts, but did not react with striated muscle myosin heavy chains. By immunofluorescence it decorated the stress fibers of well-spread cells with a characteristic striated pattern, while it did not react with myotubes containing organized myofibrils. On native ciliated cells as well as on Triton-extracted ciliated cortices from quail oviduct, this monoclonal antibody decorated the apical pole with a stronger labeling of the periphery of the apical area. Ultrastructural localization was attempted using the immunogold technique on the same preparation. Myosin was associated with a filamentous material present between striated rootlets and the proximal extremities of the basal bodies. No labeling of the basal body itself or of axoneme was observed.

scholarly journals Centrin4p, a Novel Mammalian Centrin Specifically Expressed in Ciliated Cells

2003 ◽  
Vol 14 (5) ◽  
pp. 1818-1834 ◽  
Author(s):  
Olivier Gavet ◽  
Chantale Alvarez ◽  
Patricia Gaspar ◽  
Michel Bornens
Keyword(s):  
Posttranscriptional Regulation ◽  
Splice Variants ◽  
Regulation Mechanism ◽  
Basal Bodies ◽  
Ciliated Cells ◽  
Centriole Duplication ◽  
Gene Coding ◽  
Cilia Formation ◽  
Subsequent Step ◽  
Molecular Machinery

Centriole assembly plays an important role in centrosome duplication during the cell cycle and is a prerequisite for cilia formation during the differentiation of ciliated cells. In spite of numerous investigations, the molecular machinery that governs centriole/basal body formation remains enigmatic. Recent reports suggest that the ubiquitously expressed mammalian centrins, centrin2p and centrin3p, could be involved in the centriole duplication process. To better understand the specific functions of these proteins, we performed a systematic search for novel mammalian centrins. We isolated a cDNA and the corresponding gene coding for a novel murine centrin, centrin4p, which is more closely related to centrin2p. Like centrin2p, centrin4p accumulates to centrioles and procentrioles when ectopically expressed in HeLa cells. However, centrin4p possesses two splice variants that do not localize to centrioles, suggesting a posttranscriptional regulation mechanism. We also observed that centrin4p does not share the same centriolar targeting properties with centrin2p and 3p, indicating that these proteins could recognize different centriolar partners. Centrin4 mRNA possesses a restricted expression profile and is only detected in brain, kidney, lung, and ovary. In brain, centrin4p is exclusively expressed in ependymal and choroidal ciliated cells where it is localized to basal bodies. Together, our present data suggest that centrin4p could be more specifically involved in basal bodies assembly or in a subsequent step of ciliogenesis.

scholarly journals Immunological detection of spectrin during differentiation and in mature ciliated cells from quail oviduct

1989 ◽  
Vol 93 (4) ◽  
pp. 683-690
Author(s):  
B. Chailley ◽  
T. Frappier ◽  
F. Regnouf ◽  
M.C. Laine
Keyword(s):  
Plasma Membrane ◽  
Close Contact ◽  
Apical Part ◽  
Immunogold Labeling ◽  
Apical Plasma Membrane ◽  
Basal Bodies ◽  
Ciliated Cells ◽  
Electron Immunocytochemistry ◽  
Basal Foot ◽  
The Brain

A protein that was immunologically related to the erythrocyte and brain alpha-240-subunit and to the brain beta-235-subunit of spectrin was characterized by immunoblotting and was detected by immunofluorescence in the apical part of ciliated cells from quail oviduct. After immunogold-labeling electron immunocytochemistry, spectrin was detected mainly in a fibrillar meshwork located between the proximal parts of the basal bodies. It was also observed to be in contact with the basal foot of basal bodies, but was not found to be associated with the apical plasma membrane. Cilia and microvilli were unlabeled. In contrast, spectrin was detected in close contact with the lateral plasma membrane of mature ciliated cells as well as in stem epithelial cells in unstimulated oviduct. During ciliogenesis induced by estrogen, spectrin gradually appeared at the apex of the cells as the apical cytoskeleton differentiated.

Immunocytochemical study of the formation of striated rootlets during ciliogenesis in quail oviduct

1990 ◽  
Vol 95 (3) ◽  
pp. 423-432 ◽  
Author(s):  
M. Lemullois ◽  
M.C. Marty
Keyword(s):  
Monoclonal Antibody ◽  
Polyclonal Antibody ◽  
Ciliated Cell ◽  
Apical Part ◽  
Basal Bodies ◽  
Immunocytochemical Study ◽  
Ciliated Cells ◽  
Immunogold Staining ◽  
Dense Granules ◽  
Basal Pole

In quail oviduct, a 175K (K = 10(3) Mr) protein associated with striated rootlets was previously identified by Klotz and co-workers using monoclonal antibody CC310. As this monoclonal antibody recognizes several proteins on immunoblots of ciliated cells, we prepared a polyclonal antibody monospecific to the 175K protein by intrasplenic immunization of mice. Immunofluorescence study confirmed the distribution of the 175K protein at the apical part of the ciliated cell and its absence in other epithelial cells. Immunogold staining showed that this protein was strongly associated with the fibrillar axis of striated rootlets. The absence of labeling on striation suggested that rootlets were composed of several proteins, with one group forming the fibrillar axis and the second forming the striation. The formation of striated rootlets during ciliogenesis was studied using this polyclonal antibody. The 175K protein appeared at the beginning of centriologenesis in fibrillar material located around dense granules, and then around the generative complex. The formation of rootlets began at the basal pole of migrating basal bodies. The elongation of the rootlet axes took place when basal bodies were anchored to the plasma membrane.

scholarly journals [Ciliogenesis in the mucous cells of the quail oviduct. I. Ultrastructural study in the laying quail]

1976 ◽  
Vol 71 (2) ◽  
pp. 449-459 ◽  
Author(s):  
D Sandoz ◽  
E Biosvieux-Ulrich
Keyword(s):  
Golgi Apparatus ◽  
Ultrastructural Study ◽  
The Other ◽  
Basal Bodies ◽  
Luminal Epithelium ◽  
Mucous Cells ◽  
Ciliated Cells ◽  
Laying Quail ◽  
Golgi Zone

The luminal epithelium of the oviduct (magnum) of laying quails is composed of ciliated cells and mucous cells. Ciliogenesis was observed in some of the mucous cells. Both centrioles of the diplosome migrate to the top of the cell, and one of them induces the formation of a rudimentary cilium. In some of the other cells, that are filled with mucous granules, the formation of basal bodies by an acentriolar pathway was observed. In these cells, numerous, dense fibrous masses are associated with the forming face of the Golgi apparatus. In the Golgi zone, generative complexes composed of a deuterosome and some forming procentrioles were found. Cilia develop from completed basal bodies. During ciliogenesis, the Golgi apparatus is disorganized, and generally the production of mucous granules is arrested. The nucleus is also modified: it becomes larger and the chromatin is dispersed. It is assumed that mucous cells are able to be transformed into ciliated cells in the oviduct of laying quails.

scholarly journals Rootletin Interacts with C-Nap1 and May Function as a Physical Linker between the Pair of Centrioles/Basal Bodies in Cells

2006 ◽  
Vol 17 (2) ◽  
pp. 1033-1040 ◽  
Author(s):  
Jun Yang ◽  
Michael Adamian ◽  
Tiansen Li
Keyword(s):  
Potential Role ◽  
Basal Bodies ◽  
Ciliated Cells ◽  
Centrosome Separation ◽  
Interphase Cells ◽  
Major Structural Component ◽  
Chromosome Separation ◽  
Shape And Size ◽  
In Cells

Rootletin, a major structural component of the ciliary rootlet, is located at the basal bodies and centrosomes in ciliated and nonciliated cells, respectively. Here we investigated its potential role in the linkage of basal bodies/centrioles and the mechanism involved in such linkages. We show that rootletin interacts with C-Nap1, a protein restricted at the ends of centrioles and functioning in centrosome cohesion in interphase cells. Their interaction in vivo is supported by their colocalization at the basal bodies/centrioles and coordinated association with the centrioles during the cell cycle. Ultrastructural examinations demonstrate that rootletin fibers connect the basal bodies in ciliated cells and are present both at the ends of and in between the pair of centrioles in nonciliated cells. The latter finding stands in contrast with C-Nap1, which is present only at the ends of the centrioles. Transient expression of C-Nap1 fragments dissociated rootletin fibers from the centrioles, resulting in centrosome separation in interphase. Overexpression of rootletin in cells caused multinucleation, micronucleation, and irregularity of nuclear shape and size, indicative of defects in chromosome separation. These data suggest that rootletin may function as a physical linker between the pair of basal bodies/centrioles by binding to C-Nap1.

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