scholarly journals CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaging

Optica ◽  
2020 ◽  
Vol 7 (7) ◽  
pp. 802 ◽  
Author(s):  
Michael A. Phillips ◽  
Maria Harkiolaki ◽  
David Miguel Susano Pinto ◽  
Richard M. Parton ◽  
Ana Palanca ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Super Resolution ◽  
Structured Illumination

scholarly journals Resolving the internal morphology of core–shell microgels with super-resolution fluorescence microscopy

2020 ◽  
Vol 2 (1) ◽  
pp. 323-331 ◽  
Author(s):  
Pia Otto ◽  
Stephan Bergmann ◽  
Alice Sandmeyer ◽  
Maxim Dirksen ◽  
Oliver Wrede ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Internal Structure ◽  
Single Molecule ◽  
Fluorescent Dyes ◽  
Super Resolution ◽  
Core Shell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Internal Morphology

We investigate the internal structure of smart core–shell microgels by super-resolution fluorescence microscopy by combining of 3D single molecule localization and structured illumination microscopy using freely diffusing fluorescent dyes.

Super-resolution structured illumination fluorescence microscopy of the lateral wall of the cochlea: the Connexin26/30 proteins are separately expressed in man

2016 ◽  
Vol 365 (1) ◽  
pp. 13-27 ◽  
Author(s):  
Wei Liu ◽  
Fredrik Edin ◽  
Hans Blom ◽  
Peetra Magnusson ◽  
Annelies Schrott-Fischer ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Super Resolution ◽  
Lateral Wall ◽  
Structured Illumination

scholarly journals Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications

2013 ◽  
Vol 36 (1-2) ◽  
pp. 27-35 ◽  
Author(s):  
Tieqiao Zhang ◽  
Samantha Osborn ◽  
Chloe Brandow ◽  
Denis Dwyre ◽  
Ralph Green ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Optical Microscopy ◽  
Blood Cells ◽  
Super Resolution ◽  
Structured Illumination ◽  
Tissue Sections ◽  
Moiré Effect ◽  
Different Types ◽  
Microscopy Images ◽  
Conventional Light Microscopy

Structured illumination fluorescence microscopy utilizes interfering light and the moiré effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in thexandydirections, it also allows enhancement of resolution in thez- direction by the same factor of two (to approximately 220 nm), making it a powerful tool for 3-D morphology studies of fluorescently labeled cells or thin tissue sections. In this report, we applied this technique to several types of blood cells that are commonly seen in hematopathology. Compared with standard brightfield and ordinary fluorescence microscopy images, the 3-D morphology results clearly reveal the morphological features of different types of normal blood cells. We have also used this technique to evaluate morphologies of abnormal erythrocytes and compare them with those recorded on normal cells. The results give a very intuitive presentation of morphological structures of erythrocytes with great details. This research illustrates the potential of this technique to be used in hematology and cyto-pathology studies aimed at identifying nanometer-sized features that cannot be distinguished otherwise with conventional optical microscopy.

Super-Resolution Fluorescence Microscopy Image Reconstruction Algorithm Based on Structured Illumination

2021 ◽  
Vol 48 (3) ◽  
pp. 0307001
Author(s):  
刘智 Liu Zhi ◽  
罗泽伟 Luo Zewei ◽  
王正印 Wang Zhengyin ◽  
涂壮 Tu Zhuang ◽  
庄正飞 Zhuang Zhengfei ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Fluorescence Microscopy ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Structured Illumination ◽  
Image Reconstruction Algorithm ◽  
Microscopy Image

scholarly journals Single-shot super-resolution total internal reflection fluorescence microscopy

2017 ◽  
Author(s):  
Min Guo ◽  
Panagiotis Chandris ◽  
John Paul Giannini ◽  
Adam J. Trexler ◽  
Robert Fischer ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Super Resolution ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Single Shot ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Simple Method ◽  
Close Proximity

AbstractWe demonstrate a simple method for combining instant structured illumination microscopy (SIM) with total internal reflection fluorescence microscopy (TIRF), doubling the spatial resolution of TIRF (down to 115 +/-13 nm) and enabling imaging frame rates up to 100 Hz over hundreds of time points. We apply instant TIRF-SIM to multiple live samples, achieving rapid, high contrast super-resolution imaging in close proximity to the coverslip surface.

scholarly journals Camera-based localization microscopy optimized with calibrated structured illumination

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Martin Schmidt ◽  
Adam C. Hundahl ◽  
Henrik Flyvbjerg ◽  
Rodolphe Marie ◽  
Kim I. Mortensen
Keyword(s):  
Data Analysis ◽  
Super Resolution ◽  
Wide Field ◽  
Structured Illumination ◽  
Uniform Illumination ◽  
Localization Microscopy ◽  
Localization And Tracking ◽  
Field Imaging ◽  
Wide Field Imaging ◽  
Localization Information

AbstractUntil very recently, super-resolution localization and tracking of fluorescent particles used camera-based wide-field imaging with uniform illumination. Then it was demonstrated that structured illuminations encode additional localization information in images. The first demonstration of this uses scanning and hence suffers from limited throughput. This limitation was mitigated by fusing camera-based localization with wide-field structured illumination. Current implementations, however, use effectively only half the localization information that they encode in images. Here we demonstrate how all of this information may be exploited by careful calibration of the structured illumination. Our approach achieves maximal resolution for given structured illumination, has a simple data analysis, and applies to any structured illumination in principle. We demonstrate this with an only slightly modified wide-field microscope. Our protocol should boost the emerging field of high-precision localization with structured illumination.

scholarly journals Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 861
Author(s):  
Jacopo Cardellini ◽  
Arianna Balestri ◽  
Costanza Montis ◽  
Debora Berti
Keyword(s):  
Drug Delivery ◽  
Fluorescence Microscopy ◽  
Drug Delivery Systems ◽  
Laser Scanning ◽  
Super Resolution ◽  
Laser Scanning Confocal Microscopy ◽  
Delivery Systems ◽  
Scanning Confocal Microscopy ◽  
Biological Phenomena

In the past decade(s), fluorescence microscopy and laser scanning confocal microscopy (LSCM) have been widely employed to investigate biological and biomimetic systems for pharmaceutical applications, to determine the localization of drugs in tissues or entire organisms or the extent of their cellular uptake (in vitro). However, the diffraction limit of light, which limits the resolution to hundreds of nanometers, has for long time restricted the extent and quality of information and insight achievable through these techniques. The advent of super-resolution microscopic techniques, recognized with the 2014 Nobel prize in Chemistry, revolutionized the field thanks to the possibility to achieve nanometric resolution, i.e., the typical scale length of chemical and biological phenomena. Since then, fluorescence microscopy-related techniques have acquired renewed interest for the scientific community, both from the perspective of instrument/techniques development and from the perspective of the advanced scientific applications. In this contribution we will review the application of these techniques to the field of drug delivery, discussing how the latest advancements of static and dynamic methodologies have tremendously expanded the experimental opportunities for the characterization of drug delivery systems and for the understanding of their behaviour in biologically relevant environments.

scholarly journals Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Liliana Barbieri ◽  
Huw Colin-York ◽  
Kseniya Korobchevskaya ◽  
Di Li ◽  
Deanna L. Wolfson ◽  
...  
Keyword(s):  
Resolution Enhancement ◽  
Traction Force ◽  
Super Resolution ◽  
Traction Force Microscopy ◽  
Two Dimensional ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Force Microscopy ◽  
Health And Disease ◽  
Spatio Temporal

AbstractQuantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

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