Cerebrovascular imaging in vivo by non-contact photoacoustic microscopy based on photoacoustic remote sensing with a laser diode for interrogation

2021 ◽  
Author(s):  
Siqi Liang ◽  
Jiasheng Zhou ◽  
Wenzhao Yang ◽  
Sung-Liang Chen
Keyword(s):  
Remote Sensing ◽  
Laser Diode ◽  
Photoacoustic Microscopy ◽  
Cerebrovascular Imaging

scholarly journals High-resolution in vivo imaging of rhesus cerebral cortex with ultrafast portable photoacoustic microscopy

NeuroImage ◽  
2021 ◽  
pp. 118260
Author(s):  
Wei Qin ◽  
Qi Gan ◽  
Lei Yang ◽  
Yongchao Wang ◽  
Weizhi Qi ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
High Resolution ◽  
In Vivo Imaging ◽  
Photoacoustic Microscopy

scholarly journals Recovery of photoacoustic images based on accurate ultrasound positioning

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yinhao Pan ◽  
Ningbo Chen ◽  
Liangjian Liu ◽  
Chengbo Liu ◽  
Zhiqiang Xu ◽  
...  
Keyword(s):  
Biological Tissue ◽  
Large Field ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Imaging Data ◽  
Motor Speed ◽  
Image Correction ◽  
Microscopy Imaging ◽  
Scanning Method

AbstractPhotoacoustic microscopy is an in vivo imaging technology based on the photoacoustic effect. It is widely used in various biomedical studies because it can provide high-resolution images while being label-free, safe, and harmless to biological tissue. Polygon-scanning is an effective scanning method in photoacoustic microscopy that can realize fast imaging of biological tissue with a large field of view. However, in polygon-scanning, fluctuations of the rotating motor speed and the geometric error of the rotating mirror cause image distortions, which seriously affect the photoacoustic-microscopy imaging quality. To improve the image quality of photoacoustic microscopy using polygon-scanning, an image correction method is proposed based on accurate ultrasound positioning. In this method, the photoacoustic and ultrasound imaging data of the sample are simultaneously obtained, and the angle information of each mirror used in the polygon-scanning is extracted from the ultrasonic data to correct the photoacoustic images. Experimental results show that the proposed method can significantly reduce image distortions in photoacoustic microscopy, with the image dislocation offset decreasing from 24.774 to 10.365 μm.

scholarly journals In Vitro and In Vivo Multispectral Photoacoustic Imaging for the Evaluation of Chromophore Concentration

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3366
Author(s):  
Aneline Dolet ◽  
Rita Ammanouil ◽  
Virginie Petrilli ◽  
Cédric Richard ◽  
Piero Tortoli ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Photoacoustic Imaging ◽  
Hyperspectral Remote Sensing ◽  
Reference Spectrum ◽  
In Vivo Experiments ◽  
Mouse Tumors ◽  
Chromophore Concentration ◽  
Saturation Rate

Multispectral photoacoustic imaging is a powerful noninvasive medical imaging technique that provides access to functional information. In this study, a set of methods is proposed and validated, with experimental multispectral photoacoustic images used to estimate the concentration of chromophores. The unmixing techniques used in this paper consist of two steps: (1) automatic extraction of the reference spectrum of each pure chromophore; and (2) abundance calculation of each pure chromophore from the estimated reference spectra. The compared strategies bring positivity and sum-to-one constraints, from the hyperspectral remote sensing field to multispectral photoacoustic, to evaluate chromophore concentration. Particularly, the study extracts the endmembers and compares the algorithms from the hyperspectral remote sensing domain and a dedicated algorithm for segmentation of multispectral photoacoustic data to this end. First, these strategies are tested with dilution and mixing of chromophores on colored 4% agar phantom data. Then, some preliminary in vivo experiments are performed. These consist of estimations of the oxygen saturation rate (sO2) in mouse tumors. This article proposes then a proof-of-concept of the interest to bring hyperspectral remote sensing algorithms to multispectral photoacoustic imaging for the estimation of chromophore concentration.

scholarly journals Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo

2010 ◽  
Vol 35 (19) ◽  
pp. 3195 ◽  
Author(s):  
Chi Zhang ◽  
Konstantin Maslov ◽  
Lihong V. Wang
Keyword(s):  
Optical Absorption ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Subwavelength Resolution

scholarly journals Reflection-mode in vivo photoacoustic microscopy with subwavelength lateral resolution

2014 ◽  
Vol 5 (12) ◽  
pp. 4235 ◽  
Author(s):  
Wei Song ◽  
Wei Zheng ◽  
Ruimin Liu ◽  
Riqiang Lin ◽  
Hongtao Huang ◽  
...  
Keyword(s):  
Lateral Resolution ◽  
Photoacoustic Microscopy ◽  
Reflection Mode

scholarly journals Transcranial Imaging of Functional Cerebral Hemodynamic Changes in Single Blood Vessels using in vivo Photoacoustic Microscopy

2012 ◽  
Vol 32 (6) ◽  
pp. 938-951 ◽  
Author(s):  
Lun-De Liao ◽  
Chin-Teng Lin ◽  
Yen-Yu I Shih ◽  
Timothy Q Duong ◽  
Hsin-Yi Lai ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Cerebral Blood Volume ◽  
Hemoglobin Concentration ◽  
Photoacoustic Microscopy ◽  
Functional Changes ◽  
Sagittal Sinus ◽  
Microscopy Technique ◽  
Initial Dip ◽  
Transcranial Imaging

Optical imaging of changes in total hemoglobin concentration ( HbT), cerebral blood volume ( CBV), and hemoglobin oxygen saturation ( SO 2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel functional photoacoustic microscopy technique to probe the responses of single cortical vessels to left forepaw electrical stimulation in mice with intact skulls. Functional changes in HbT, CBV, and SO 2 in the superior sagittal sinus and different-sized arterioles from the anterior cerebral artery system were bilaterally imaged with unambiguous 36 × 65- μm2 spatial resolution. In addition, an early decrease of SO 2 in single blood vessels during activation (i.e., ‘the initial dip’) was observed. Our results indicate that the initial dip occurred specifically in small arterioles of activated regions but not in large veins. This technique complements other existing imaging approaches for the investigation of the hemodynamic responses in single cerebral blood vessels.

scholarly journals In vivo fast variable focus photoacoustic microscopy using an electrically tunable lens

2014 ◽  
Vol 22 (17) ◽  
pp. 20130 ◽  
Author(s):  
Bingbing Li ◽  
Huan Qin ◽  
Sihua Yang ◽  
Da Xing
Keyword(s):  
Fast Variable ◽  
Photoacoustic Microscopy ◽  
Variable Focus ◽  
Tunable Lens

scholarly journals High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Xiufeng Li ◽  
Victor T C Tsang ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T W Wong
Keyword(s):  
High Resolution ◽  
High Speed ◽  
High Performance ◽  
Continuous Wave ◽  
Signal To Noise Ratio ◽  
Cost Effective ◽  
High Signal ◽  
Photoacoustic Microscopy ◽  
Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

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