Wide-field optical coherence microscopy of the mouse brain slice

2015 ◽  
Vol 40 (19) ◽  
pp. 4420 ◽  
Author(s):  
Eunjung Min ◽  
Junwon Lee ◽  
Andrey Vavilin ◽  
Sunwoo Jung ◽  
Sungwon Shin ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Brain Slice ◽  
Wide Field ◽  
Optical Coherence ◽  
Optical Coherence Microscopy

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

Wide-field, full-field optical coherence microscopy for high-axial-resolution phase and amplitude imaging

2015 ◽  
Vol 54 (27) ◽  
pp. 8212 ◽  
Author(s):  
Antoine Federici ◽  
Henrique S. Gutierrez da Costa ◽  
Jonas Ogien ◽  
Audrey K. Ellerbee ◽  
Arnaud Dubois
Keyword(s):  
Wide Field ◽  
Optical Coherence ◽  
Axial Resolution ◽  
Optical Coherence Microscopy ◽  
Full Field

scholarly journals Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus optical coherence microscopy

2016 ◽  
Author(s):  
Szymon Tamborski ◽  
Hong Chou Lyu ◽  
Danuta Bukowska ◽  
Hubert Dolezyczek ◽  
Grzegorz Wilczynski ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Vascular System ◽  
Optical Coherence ◽  
Optical Coherence Microscopy

3D wide field-of-view Gabor-domain optical coherence microscopy advancing real-time in-vivo imaging and metrology

2017 ◽  
Author(s):  
Cristina Canavesi ◽  
Andrea Cogliati ◽  
Adam Hayes ◽  
Patrice Tankam ◽  
Anand Santhanam ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Field Of View ◽  
Wide Field ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Wide Field Of View ◽  
Gabor Domain

scholarly journals Computed optical coherence microscopy of mouse brain ex vivo

2019 ◽  
Vol 24 (11) ◽  
pp. 1 ◽  
Author(s):  
Meiqi Wu ◽  
David M. Small ◽  
Nozomi Nishimura ◽  
Steven G. Adie
Keyword(s):  
Mouse Brain ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Optical Coherence Microscopy
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