scholarly journals Total internal reflection enabled wide-field coherent anti-Stokes Raman scattering microscopy

2020 ◽  
Vol 45 (11) ◽  
pp. 3087
Author(s):  
Benjamin Doughty ◽  
Uvinduni I. Premadasa ◽  
John F. Cahill ◽  
Amber B. Webb ◽  
Jennifer L. Morrell-Falvey ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Wide Field ◽  
Anti Stokes

scholarly journals Total internal reflection enabled wide-field coherent anti-Stokes Raman scattering microscopy

2020 ◽  
Vol 45 (11) ◽  
pp. 3087
Author(s):  
Benjamin Doughty ◽  
Uvinduni I. Premadasa ◽  
John F. Cahill ◽  
Amber B. Webb ◽  
Jennifer L. Morrell-Falvey ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Wide Field ◽  
Anti Stokes

Wide-field coherent anti-Stokes Raman scattering microscopy

2004 ◽  
Vol 84 (5) ◽  
pp. 816-818 ◽  
Author(s):  
Christoph Heinrich ◽  
Stefan Bernet ◽  
Monika Ritsch-Marte
Keyword(s):  
Raman Scattering ◽  
Wide Field ◽  
Anti Stokes

scholarly journals Wide-field coherent anti-Stokes Raman scattering microscopy based on picosecond supercontinuum source

APL Photonics ◽  
2018 ◽  
Vol 3 (11) ◽  
pp. 116104 ◽  
Author(s):  
Yujie Shen ◽  
Jizhou Wang ◽  
Kai Wang ◽  
Alexei V. Sokolov ◽  
Marlan O. Scully
Keyword(s):  
Raman Scattering ◽  
Wide Field ◽  
Anti Stokes

Measurement of Ca2+ signaling dynamics in exocrine cells with total internal reflection microscopy

2006 ◽  
Vol 291 (1) ◽  
pp. G146-G155 ◽  
Author(s):  
Jong Hak Won ◽  
David I. Yule
Keyword(s):  
Plasma Membrane ◽  
Total Internal Reflection ◽  
Acinar Cells ◽  
Internal Reflection ◽  
Pancreatic Acinar Cells ◽  
Wide Field ◽  
Exocrine Cells ◽  
Parotid Acinar Cells ◽  
Signaling Dynamics ◽  
Total Internal Reflection Microscopy

In nonexcitable cells, such as exocrine cells from the pancreas and salivary glands, agonist-stimulated Ca2+ signals consist of both Ca2+ release and Ca2+ influx. We have investigated the contribution of these processes to membrane-localized Ca2+ signals in pancreatic and parotid acinar cells using total internal reflection fluorescence (TIRF) microscopy (TIRFM). This technique allows imaging with unsurpassed resolution in a limited zone at the interface of the plasma membrane and the coverslip. In TIRFM mode, physiological agonist stimulation resulted in Ca2+ oscillations in both pancreas and parotid with qualitatively similar characteristics to those reported using conventional wide-field microscopy (WFM). Because local Ca2+ release in the TIRF zone would be expected to saturate the Ca2+ indicator (Fluo-4), these data suggest that Ca2+ release is occurring some distance from the area subjected to the measurement. When acini were stimulated with supermaximal concentrations of agonists, an initial peak, largely due to Ca2+ release, followed by a substantial, maintained plateau phase indicative of Ca2+ entry, was observed. The contribution of Ca2+ influx and Ca2+ release in isolation to these near-plasma membrane Ca2+ signals was investigated by using a Ca2+ readmission protocol. In the absence of extracellular Ca2+, the profile and magnitude of the initial Ca2+ release following stimulation with maximal concentrations of agonist or after SERCA pump inhibition were similar to those obtained with WFM in both pancreas and parotid acini. In contrast, when Ca2+ influx was isolated by subsequent Ca2+ readmission, the Ca2+ signals evoked were more robust than those measured with WFM. Furthermore, in parotid acinar cells, Ca2+ readdition often resulted in the apparent saturation of Fluo-4 but not of the low-affinity dye Fluo-4-FF. Interestingly, Ca2+ influx as measured by this protocol in parotid acinar cells was substantially greater than that initiated in pancreatic acinar cells. Indeed, robust Ca2+ influx was observed in parotid acinar cells even at low physiological concentrations of agonist. These data indicate that TIRFM is a useful tool to monitor agonist-stimulated near-membrane Ca2+ signals mediated by Ca2+ influx in exocrine acinar cells. In addition, TIRFM reveals that the extent of Ca2+ influx in parotid acinar cells is greater than pancreatic acinar cells when compared using identical methodologies.

scholarly journals Wide-field Surface-Enhanced Coherent Anti-Stokes Raman Scattering Microscopy

2021 ◽  
Author(s):  
Cheng Zong ◽  
Ran Cheng ◽  
Fukai Chen ◽  
Peng Lin ◽  
Meng Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Large Scale ◽  
Time Lapse ◽  
Live Cells ◽  
Large Field ◽  
Wide Field ◽  
Label Free ◽  
Label Free Detection ◽  
Surface Enhanced ◽  
Anti Stokes

Surface-enhanced Raman scattering (SERS) spectroscopy has been used extensively to study biology, chemistry, and materials. However, a point-by-point SERS mapping is time-consuming, taking minutes to hours for large-scale imaging. Here, we report a wide-field surface-enhanced coherent anti-Stokes Raman scattering (WISE-CARS) microscopy for monitoring nanotags in live cells and label-free detection of metabolic molecules. The WISE-CARS microscope achieves an imaging speed as fast as 120 frames per second for a large field of view of 130 microns X 130 microns. By spectral focusing of femtosecond lasers, a hyperspectral WISE-CARS stack of 120 frames can be acquired with a spectral resolution of 10 cm-1, where over 1 million Raman spectra are parallelly recorded within 0.5 seconds. As applications, we demonstrate time-lapse, 3D WISE-CARS imaging of nanotags in live cells as well as label-free detection of adenine released from S. aureus.

Wide-Field Vibrational Phase Contrast Imaging Based on Coherent Anti-Stokes Raman Scattering Holography

2015 ◽  
Vol 32 (7) ◽  
pp. 074209
Author(s):  
Yong-Gang Lv ◽  
Zi-Heng Ji ◽  
Da-Shan Dong ◽  
Ke-Bin Shi ◽  
Qi-Huang Gong
Keyword(s):  
Raman Scattering ◽  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Wide Field ◽  
Contrast Imaging ◽  
Anti Stokes
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