Through-focus scanning-optical-microscope imaging method for nanoscale dimensional analysis

2008 ◽  
Vol 33 (17) ◽  
pp. 1990 ◽  
Author(s):  
Ravikiran Attota ◽  
Thomas A. Germer ◽  
Richard M. Silver
Keyword(s):  
Dimensional Analysis ◽  
Optical Microscope ◽  
Imaging Method ◽  
Microscope Imaging

Measurement of Electrical Impedance and Eddy Currents in Tissue Phantoms

2013 ◽  
Author(s):  
Emily Sequin ◽  
Karen Bellman ◽  
Scott Koch ◽  
Joseph West ◽  
Shaurya Prakash ◽  
...  
Keyword(s):  
Eddy Currents ◽  
Clinical Medicine ◽  
Frozen Section ◽  
Optical Microscope ◽  
Imaging Method ◽  
Tissue Properties ◽  
Tissue Phantoms ◽  
Frozen Section Analysis ◽  
Resected Tissue ◽  
Current Characteristics

Measurement of the electromagnetic (EM) properties of tissue such as electrical conductivity, permittivity, and eddy current characteristics can be used in clinical medicine for characterizing and distinguishing soft tissue morphology. Such measurements can yield complementary information to what can be obtained using analysis with an optical microscope. An example is the assessment of margins during the surgical resection of occult tumors. In current practice, the surgeon relies on pre-operative imaging modalities, sight and palpation to locate and attempt to fully resect the tumor(s). Frozen section pathological assessment offers the only other resource available to the surgeon for margin analysis, but it is incomplete because only a small fraction of the resected tissue is examined and it is often not feasible to wait for the results of the frozen section analysis before completing the surgery. This paper describes a characterization and imaging method based on variations in electromagnetic tissue properties to assess the surgical margins of resected tissues. This is noteworthy because accurate margin assessment has been shown to significantly improve long term patient outcomes[1].

Abrasive Surfaces Measured by Digital Optical Stereopsis

2009 ◽  
Vol 76-78 ◽  
pp. 465-470
Author(s):  
Dennis V. de Pellegrin ◽  
Andrew Torrance
Keyword(s):  
Failure Analysis ◽  
Digital Elevation Models ◽  
Optical Microscope ◽  
The Other ◽  
Digital Elevation ◽  
Microscope Imaging

The principle of stereopsis involves measuring an object’s geometry from a pair of images taken at slightly different viewing positions. This technique is frequently used for geographical mapping in satellite-based reconnaissance, however, the same practice has not been reliably applied at the other end of the scale spectrum: i.e. optical microscope imaging. The impediments have been identified and addressed in this work, concluding that optical stereopsis can be applied to microscopical surface examinations, and that the resulting digital elevation models can be of particular use in tribological investigations for performance and failure analysis.

Electron microscopy of biological specimens by means of an electrostatic phase plate

1972 ◽  
Vol 329 (1578) ◽  
pp. 327-359 ◽  
Keyword(s):  
Spherical Aberration ◽  
Special Property ◽  
Field Method ◽  
Objective Lens ◽  
Phase Plate ◽  
Imaging Method ◽  
Contrast Effects ◽  
Resolution Limit ◽  
Bright Field ◽  
Microscope Imaging

A new electron microscope imaging method has been developed that is especially suited to the study of thin biological materials. It involves the use of an electrostatic phase plate - a device which creates a more or less uniform difference in optical path between the un­scattered and scattered waves by means of its electric field. This phase plate functions in an analogous manner to the absorbing bright contrast phase plate of light microscopy. The contrast effects and aberrations peculiar to the method have been examined and are discussed in terms of their likely influence on the image’s representation of the object structure. Analysis of electron micrographs of some biological test specimens, whose structure is relatively well known, confirms that this representation, to a resolution of ca . 0.85 nm, is a particularly faithful one. In the analysis the resolution limit was determined by the degree of specimen preservation, and a real limit, determined by the degree of spherical aberration in the objective lens, of ca . 0.5 nm is expected. A special property of the imaging method, as distinct from the conventional bright field method, is that it emphasizes the detail within the biological material itself, but reduces the contrast from the surrounding film of stain; negative staining remains necessary only because it helps to preserve the morphology of the specimen during irradiation. Evidence is presented that this property enables the method to display information about the specimen that it would not be possible to detect with the bright field method.

Tribological Process Characterization With In-Situ Quantitative Nano-Scale Metrology

2005 ◽  
Author(s):  
Antanas Daugela ◽  
Alex Meyman ◽  
Vladimir Knyazik ◽  
Nikolai Yeremin
Keyword(s):  
Mechanical Properties ◽  
Finite Element ◽  
Finite Element Model ◽  
Element Model ◽  
Optical Microscope ◽  
Nano Scale ◽  
Process Characterization ◽  
Microscope Imaging

A novel quantitative nano+micro-tribometer with integrated nanoindenter, SPM and optical microscope imaging has been used to characterize mechanical properties of Cu coated Si wafers at various test stages. A 2D Finite Element Model was developed to study changes on workhardened contacts assessed via nanoindentation experiments.

Acousto-Optic Tunable Filter Hyperspectral Microscope Imaging Method for Characterizing Spectra from Foodborne Pathogens

2012 ◽  
Vol 55 (5) ◽  
pp. 1997-2006 ◽  
Author(s):  
B. Park ◽  
S. C. Yoon ◽  
S. Lee ◽  
J. Sundaram ◽  
W. R. Windham ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Tunable Filter ◽  
Imaging Method ◽  
Microscope Imaging

A New Electron Microscope Imaging Method for Enhancing Detail in Thin Biological Specimens

1974 ◽  
Vol 29 (1) ◽  
pp. 158-163 ◽  
Author(s):  
P. N. T. Unwin
Keyword(s):  
Electron Microscope ◽  
Field Method ◽  
Objective Lens ◽  
Phase Plate ◽  
Imaging Method ◽  
Bright Field ◽  
Realistic Representation ◽  
Microscope Imaging ◽  
Electron Microscope Imaging ◽  
Strong Contrast

An electron microscope imaging method is described which makes use of an electrostatic device, analogous in function to the absorbing phase plate of light microscope, to produce strong contrast in biological specimens. This device is situated at the back focal plane of the objective lens in place of a normal objective aperture. The images created provide an especially realistic representation of the specimen structure and contain information about it that would not necessarily be able to be detected by the conventional bright field method of observation.

scholarly journals Confocal microscopy 3D imaging of diesel particulate matter

2021 ◽  
Author(s):  
Lisa Miyashita ◽  
Gary Foley ◽  
Ian Gill ◽  
Gavin Gillmore ◽  
Jonathan Grigg ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Confocal Microscopy ◽  
Major Axis ◽  
Lung Epithelial Cells ◽  
Imaging Method ◽  
Diesel Particulate Matter ◽  
Diesel Particulate ◽  
New Findings ◽  
Microscope Imaging ◽  
Axis Length

AbstractTo date, diesel particulate matter (DPM) has been described as aggregates of spherule particles with a smooth appearing surface. We have used a new colour confocal microscope imaging method to study the 3D shape of diesel particulate matter (DPM); we observed that the particles can have sharp jagged appearing edges and consistent with these findings, 2D light microscopy demonstrated that DPM adheres to human lung epithelial cells. Importantly, the slide preparation and confocal microscopy method applied avoids possible alteration to the particles’ surfaces and enables colour 3D visualisation of the particles. From twenty-one PM10 particles, the mean (standard deviation) major axis length was 5.6 (2.25) μm with corresponding values for the minor axis length of 3.8 (1.25) μm. These new findings may help explain why air pollution particulate matter (PM) has the ability to infiltrate human airway cells, potentially leading to respiratory tract, cardiovascular and neurological disease.

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