Acousto-Optic Tunable Filter Hyperspectral Microscope Imaging Method for Characterizing Spectra from Foodborne Pathogens

2012 ◽  
Vol 55 (5) ◽  
pp. 1997-2006 ◽  
Author(s):  
B. Park ◽  
S. C. Yoon ◽  
S. Lee ◽  
J. Sundaram ◽  
W. R. Windham ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Tunable Filter ◽  
Imaging Method ◽  
Microscope Imaging

Electron microscopy of biological specimens by means of an electrostatic phase plate

1972 ◽  
Vol 329 (1578) ◽  
pp. 327-359 ◽  
Keyword(s):  
Spherical Aberration ◽  
Special Property ◽  
Field Method ◽  
Objective Lens ◽  
Phase Plate ◽  
Imaging Method ◽  
Contrast Effects ◽  
Resolution Limit ◽  
Bright Field ◽  
Microscope Imaging

A new electron microscope imaging method has been developed that is especially suited to the study of thin biological materials. It involves the use of an electrostatic phase plate - a device which creates a more or less uniform difference in optical path between the un­scattered and scattered waves by means of its electric field. This phase plate functions in an analogous manner to the absorbing bright contrast phase plate of light microscopy. The contrast effects and aberrations peculiar to the method have been examined and are discussed in terms of their likely influence on the image’s representation of the object structure. Analysis of electron micrographs of some biological test specimens, whose structure is relatively well known, confirms that this representation, to a resolution of ca . 0.85 nm, is a particularly faithful one. In the analysis the resolution limit was determined by the degree of specimen preservation, and a real limit, determined by the degree of spherical aberration in the objective lens, of ca . 0.5 nm is expected. A special property of the imaging method, as distinct from the conventional bright field method, is that it emphasizes the detail within the biological material itself, but reduces the contrast from the surrounding film of stain; negative staining remains necessary only because it helps to preserve the morphology of the specimen during irradiation. Evidence is presented that this property enables the method to display information about the specimen that it would not be possible to detect with the bright field method.

A New Electron Microscope Imaging Method for Enhancing Detail in Thin Biological Specimens

1974 ◽  
Vol 29 (1) ◽  
pp. 158-163 ◽  
Author(s):  
P. N. T. Unwin
Keyword(s):  
Electron Microscope ◽  
Field Method ◽  
Objective Lens ◽  
Phase Plate ◽  
Imaging Method ◽  
Bright Field ◽  
Realistic Representation ◽  
Microscope Imaging ◽  
Electron Microscope Imaging ◽  
Strong Contrast

An electron microscope imaging method is described which makes use of an electrostatic device, analogous in function to the absorbing phase plate of light microscope, to produce strong contrast in biological specimens. This device is situated at the back focal plane of the objective lens in place of a normal objective aperture. The images created provide an especially realistic representation of the specimen structure and contain information about it that would not necessarily be able to be detected by the conventional bright field method of observation.

scholarly journals Confocal microscopy 3D imaging of diesel particulate matter

2021 ◽  
Author(s):  
Lisa Miyashita ◽  
Gary Foley ◽  
Ian Gill ◽  
Gavin Gillmore ◽  
Jonathan Grigg ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Confocal Microscopy ◽  
Major Axis ◽  
Lung Epithelial Cells ◽  
Imaging Method ◽  
Diesel Particulate Matter ◽  
Diesel Particulate ◽  
New Findings ◽  
Microscope Imaging ◽  
Axis Length

AbstractTo date, diesel particulate matter (DPM) has been described as aggregates of spherule particles with a smooth appearing surface. We have used a new colour confocal microscope imaging method to study the 3D shape of diesel particulate matter (DPM); we observed that the particles can have sharp jagged appearing edges and consistent with these findings, 2D light microscopy demonstrated that DPM adheres to human lung epithelial cells. Importantly, the slide preparation and confocal microscopy method applied avoids possible alteration to the particles’ surfaces and enables colour 3D visualisation of the particles. From twenty-one PM10 particles, the mean (standard deviation) major axis length was 5.6 (2.25) μm with corresponding values for the minor axis length of 3.8 (1.25) μm. These new findings may help explain why air pollution particulate matter (PM) has the ability to infiltrate human airway cells, potentially leading to respiratory tract, cardiovascular and neurological disease.

Through-focus scanning-optical-microscope imaging method for nanoscale dimensional analysis

2008 ◽  
Vol 33 (17) ◽  
pp. 1990 ◽  
Author(s):  
Ravikiran Attota ◽  
Thomas A. Germer ◽  
Richard M. Silver
Keyword(s):  
Dimensional Analysis ◽  
Optical Microscope ◽  
Imaging Method ◽  
Microscope Imaging

Weak Beam Imaging and Diffraction Studies of Stacking Faults in Silicon

1976 ◽  
Vol 34 ◽  
pp. 590-591
Author(s):  
T. Y. Tan ◽  
W. K. Tice
Keyword(s):  
Fast Neutron ◽  
Rapid Determination ◽  
Stacking Faults ◽  
Imaging Method ◽  
Large Reduction ◽  
Dislocation Loops ◽  
Fringe Spacing ◽  
Weak Beam ◽  
Kinematical Theory

In studying ion implanted semiconductors and fast neutron irradiated metals, the need for characterizing small dislocation loops having diameters of a few hundred angstrom units usually arises. The weak beam imaging method is a powerful technique for analyzing these loops. Because of the large reduction in stacking fault (SF) fringe spacing at large sg, this method allows for a rapid determination of whether the loop is faulted, and, hence, whether it is a perfect or a Frank partial loop. This method was first used by Bicknell to image small faulted loops in boron implanted silicon. He explained the fringe spacing by kinematical theory, i.e., ≃l/(Sg) in the fault fringe in depth oscillation. The fault image contrast formation mechanism is, however, really more complicated.

Performance and applications of the holography electron microscope

1993 ◽  
Vol 51 ◽  
pp. 1078-1079
Author(s):  
Akira Tonomura
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Phase Measurement ◽  
Electron Holography ◽  
Imaging Method ◽  
High Contrast ◽  
Magnetic Lens ◽  
High Brightness ◽  
Holographic Imaging ◽  
Dynamic Observation

Electron holography is a two-step imaging method. However, the ultimate performance of holographic imaging is mainly determined by the brightness of the electron beam used in the hologram-formation process. In our 350kV holography electron microscope (see Fig. 1), the decrease in the inherently high brightness of field-emitted electrons is minimized by superposing a magnetic lens in the gun, for a resulting value of 2 × 109 A/cm2 sr. This high brightness has lead to the following distinguished features. The minimum spacing (d) of carrier fringes is d = 0.09 Å, thus allowing a reconstructed image with a resolution, at least in principle, as high as 3d=0.3 Å. The precision in phase measurement can be as high as 2π/100, since the position of fringes can be known precisely from a high-contrast hologram formed under highly collimated illumination. Dynamic observation becomes possible because the current density is high.

Fast low-radiation Flash-CT-scan - An alternative imaging method in patients with congenital heart disease?

2011 ◽  
Vol 59 (S 01) ◽  
Author(s):  
S Ihlenburg ◽  
A Rüffer ◽  
T Radkow ◽  
A Purbojo ◽  
M Glöckler ◽  
...  
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Ct Scan ◽  
Congenital Heart ◽  
Imaging Method
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