scholarly journals Simulation Study on the Enhancement of Resonance Energy Transfer through Surface Plasmon Coupling in a GaN Porous Structure

2021 ◽  
Author(s):  
Yang Kuo ◽  
You-Jui Lu ◽  
Chun-Yen Shih ◽  
Chih-Chung Yang
Keyword(s):  
Energy Transfer ◽  
Porous Structure ◽  
Surface Plasmon ◽  
Simulation Study ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Plasmon Coupling ◽  
Surface Plasmon Coupling

scholarly journals Discovering Severe Acute Respiratory Syndrome Coronavirus 3CL Protease Inhibitors: Virtual Screening, Surface Plasmon Resonance, and Fluorescence Resonance Energy Transfer Assays

2006 ◽  
Vol 11 (8) ◽  
pp. 915-921 ◽  
Author(s):  
Lili Chen ◽  
Shuai Chen ◽  
Chunshan Gui ◽  
Jianhua Shen ◽  
Xu Shen ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Surface Plasmon Resonance ◽  
Virtual Screening ◽  
Severe Acute Respiratory Syndrome ◽  
Fluorescence Resonance Energy Transfer ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Resonance

An integrated system has been developed for discovering potent inhibitors of severe acute respiratory syndrome coronavirus 3C-like protease (SARS-CoV 3CLpro) by virtual screening correlating with surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) technologies-based assays. The authors screened 81,287 small molecular compounds against SPECS database by virtual screening; 256 compounds were subsequently selected for biological evaluation. Through SPR technology-based assay, 52 from these 256 compounds were discovered to show binding to SARS-CoV 3CLpro. The enzymatic inhibition activities of these 52 SARS-CoV 3CLpro binders were further applied to FRET-based assay, and IC50 values were determined. Based on this integrated assay platform, 8 new SARS-CoV 3CLpro inhibitors were discovered. The fact that the obtained IC50 values for the inhibitors are in good accordance with the discovered dissociation equilibrium constants (KDs) assayed by SPR implied the reliability of this platform. Our current work is hoped to supply a powerful approach in the discovery of potent SARS-CoV 3CLpro inhibitors, and the determined inhibitors could be used as possible lead compounds for further research.

Surface plasmon enhanced Förster resonance energy transfer between the CdTe quantum dots

2008 ◽  
Vol 93 (12) ◽  
pp. 123102 ◽  
Author(s):  
Vamsi K. Komarala ◽  
A. Louise Bradley ◽  
Yury P. Rakovich ◽  
Stephen J. Byrne ◽  
Yurii K. Gun’ko ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Surface Plasmon ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Cdte Quantum Dots ◽  
Förster Resonance Energy Transfer ◽  
Förster Resonance

scholarly journals Surface plasmon enhanced energy transfer between gold nanorods and fluorophores: application to endocytosis study and RNA detection

2015 ◽  
Vol 178 ◽  
pp. 383-394 ◽  
Author(s):  
Yinan Zhang ◽  
Guoke Wei ◽  
Jun Yu ◽  
David J. S. Birch ◽  
Yu Chen
Keyword(s):  
Energy Transfer ◽  
Surface Plasmon ◽  
Fluorescence Lifetime ◽  
Gold Nanorods ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Diagnosis And Prognosis ◽  
Photon Excitation

Previously we have demonstrated surface plasmon enhanced energy transfer between fluorophores and gold nanorods under two-photon excitation using fluorescence lifetime imaging microscopy (FLIM) in both solution and intracellular phases. These studies demonstrated that gold nanoparticle–dye energy transfer combinations are appealing, not only in Förster resonance energy transfer (FRET) imaging, but also energy transfer-based fluorescence lifetime sensing of bio-analytes. Here, we apply this approach to study the internalization of gold nanorods (GNRs) in HeLa cells using the early endosome labeling marker GFP. The observed energy transfer between GFP and the GNRs indicates the involvement of endocytosis in GNR uptake. Moreover, a novel nanoprobe based on oligonucleotide functionalized gold nanorods for nucleic acid sensing via dye–GNRs energy transfer is demonstrated, potentially opening up new possibilities in cancer diagnosis and prognosis. The influence of oligonucleotide design on such nanoprobe performance was studied for the first time using time-resolved fluorescence spectroscopy, bringing new insights to the optimization of the nanoprobe.

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