scholarly journals Axially resolved volumetric two-photon microscopy with an extended field of view using depth localization under mirrored Airy beams

2020 ◽  
Vol 28 (26) ◽  
pp. 39563
Author(s):  
Ryan K. Y. Chan ◽  
Hongsen He ◽  
Yu-Xuan Ren ◽  
Cora S. W. Lai ◽  
Edmund Y. Lam ◽  
...  
2021 ◽  
Author(s):  
Gabriel Paiva Fonseca ◽  
Matthias Baer‐Beck ◽  
Eric Fournie ◽  
Christian Hofmann ◽  
Ilaria Rinaldi ◽  
...  

2021 ◽  
Vol 18 (2) ◽  
pp. 220-220
Author(s):  
Weijian Zong ◽  
Runlong Wu ◽  
Shiyuan Chen ◽  
Junjie Wu ◽  
Hanbin Wang ◽  
...  

2007 ◽  
Author(s):  
Holger Kunze ◽  
Wolfgang Härer ◽  
Karl Stierstorfer

2008 ◽  
Vol 190 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Eoin C. Kavanagh ◽  
George Koulouris ◽  
Laurence Parker ◽  
William B. Morrison ◽  
Diane Bergin ◽  
...  

2016 ◽  
Vol 7 (2) ◽  
pp. 512 ◽  
Author(s):  
F. Momey ◽  
J.-G. Coutard ◽  
T. Bordy ◽  
F. Navarro ◽  
M. Menneteau ◽  
...  

2005 ◽  
Vol 288 (6) ◽  
pp. F1084-F1089 ◽  
Author(s):  
Bruce A. Molitoris ◽  
Ruben M. Sandoval

Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within the same field of view over seconds to weeks. This approach invigorates the validity of data and facilitates analysis and interpretation as trends are more readily determined when one is more closely monitoring indicative physiological parameters. Therefore, in this review we emphasize how specific approaches will enable studies into glomerular permeability, proximal tubule endocytosis, and microvascular function within the kidney. We attempt to show how visual data can be quantified, thus allowing enhanced understanding of the process under study. Finally, emphasis is given to the possible future opportunities of this technology and its present limitations.


2020 ◽  
Vol 22 (2) ◽  
pp. 152 ◽  
Author(s):  
Chrysostomos Sahinis ◽  
Eleftherios Kellis ◽  
Nikiforos Galanis ◽  
Konstantinos Dafkou ◽  
Athanasios Ellinoudis

Aim: Τo examine the inter- and intra-muscular differences in the anatomical cross-sectional area (CSA) of the quadricep muscles, using extended - field of view (EFOV) ultrasonography (US).Material and methods: Panoramic transverse US images of the thigh were acquired from 10 young participants at five different locations across the thigh, in two sessions, spaced a week apart. The CSA of the vastus medialis (VM), rectus femoris (RF), vastus intermedius (VI), vastus lateralis (VL) and tensor vastus intermedius (TVI) was quantified.Results: The intraclass correlation coefficients ranged from 0.75 to 0.97 and the standard error of measurement ranged from 0.78% to 6.61%, indicating high test-retest reliability. Analysis of the variance indicated that among the 5 quadriceps muscles the VL and the RF displayed the greater CSA proximally, the VI medially and the VM distally across the thigh (p <0.05). No differences in the quadriceps CSA measured with and without including the TVI were found (p >0.05).Conclusions: The EFOV US technique provides transverse scans of the quadriceps muscle in vivo and allowed a reliable and non-invasive determination of CSA at a low cost. Evaluation of CSA along the thigh largely depends on the measurement site. Future studies that examine the quadriceps CSA using EFOV after any form of intervention should consider changes of at least 6.5% as meaningful.


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