scholarly journals Adaptive optics via pupil ring segmentation improves spherical aberration correction for two-photon imaging of optically cleared tissues

2020 ◽  
Vol 28 (23) ◽  
pp. 34935
Author(s):  
Yufeng Gao ◽  
Lina Liu ◽  
Yixuan Yin ◽  
Jiuling Liao ◽  
Jia Yu ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Spherical Aberration ◽  
Aberration Correction ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Multidither coherent optical adaptive technique for deep tissue two-photon microscopy

2019 ◽  
Vol 12 (04) ◽  
pp. 1942003 ◽  
Author(s):  
Biwei Zhang ◽  
Wei Gong ◽  
Chenxue Wu ◽  
Lejia Hu ◽  
Xinpei Zhu ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Diffraction Theory ◽  
Fluorescence Signal ◽  
Two Photon Microscopy ◽  
Scalar Diffraction ◽  
Two Photon ◽  
Scalar Diffraction Theory ◽  
Adaptive Technique ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging. Multidither coherent optical adaptive technique (COAT) can correct the scattered wavefront in parallel. However, the determination of the corrective phases may not be completely accurate using conventional method, which undermines the performance of this technique. In this paper, we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fluorescence signal. A numerical simulation model is established to study the performance of adaptive optics in two-photon microscopy by combining scalar diffraction theory with vector diffraction theory. The results show that the distortion of the wavefront can be corrected more thoroughly with our method in two-photon imaging. In our simulation, with the scattering from a 450-[Formula: see text]m-thick mouse brain tissue, excitation focal spots with higher peak-to-background ratio (PBR) and images with higher contrast can be obtained. Hence, further enhancement of the multidither COAT correction performance in two-photon imaging can be expected.

scholarly journals Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs

2003 ◽  
Author(s):  
Chih-kuan Tung ◽  
Tsung-Kai Chiu ◽  
Wen Lo ◽  
Po-Hsiang Wang ◽  
Sun-Jan Lin ◽  
...  
Keyword(s):  
Spherical Aberration ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Two photon imaging of mouse retina with sensorless adaptive optics

2016 ◽  
Author(s):  
Daniel J. Wahl ◽  
Michelle Cua ◽  
Sujin Lee ◽  
Yuan Zhao ◽  
Robert J. Zawadzki ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Mouse Retina ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Progress on Bimodal Adaptive Optics OCT and Two-Photon Imaging

2021 ◽  
Author(s):  
William Newberry ◽  
Laura Vargas ◽  
Marinko V. Sarunic
Keyword(s):  
Adaptive Optics ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Biocompatible well-defined chromophore–polymer conjugates for photodynamic therapy and two-photon imaging

2013 ◽  
Vol 4 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Cristina Cepraga ◽  
Thibault Gallavardin ◽  
Sophie Marotte ◽  
Pierre-Henri Lanoë ◽  
Jean-Christophe Mulatier ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Two Photon ◽  
Polymer Conjugates ◽  
Photon Imaging ◽  
Two Photon Imaging
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