Two photon imaging of mouse retina with sensorless adaptive optics

2016 ◽  
Author(s):  
Daniel J. Wahl ◽  
Michelle Cua ◽  
Sujin Lee ◽  
Yuan Zhao ◽  
Robert J. Zawadzki ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Mouse Retina ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Multidither coherent optical adaptive technique for deep tissue two-photon microscopy

2019 ◽  
Vol 12 (04) ◽  
pp. 1942003 ◽  
Author(s):  
Biwei Zhang ◽  
Wei Gong ◽  
Chenxue Wu ◽  
Lejia Hu ◽  
Xinpei Zhu ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Diffraction Theory ◽  
Fluorescence Signal ◽  
Two Photon Microscopy ◽  
Scalar Diffraction ◽  
Two Photon ◽  
Scalar Diffraction Theory ◽  
Adaptive Technique ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging. Multidither coherent optical adaptive technique (COAT) can correct the scattered wavefront in parallel. However, the determination of the corrective phases may not be completely accurate using conventional method, which undermines the performance of this technique. In this paper, we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fluorescence signal. A numerical simulation model is established to study the performance of adaptive optics in two-photon microscopy by combining scalar diffraction theory with vector diffraction theory. The results show that the distortion of the wavefront can be corrected more thoroughly with our method in two-photon imaging. In our simulation, with the scattering from a 450-[Formula: see text]m-thick mouse brain tissue, excitation focal spots with higher peak-to-background ratio (PBR) and images with higher contrast can be obtained. Hence, further enhancement of the multidither COAT correction performance in two-photon imaging can be expected.

scholarly journals Two-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina

2013 ◽  
Vol 33 (27) ◽  
pp. 10972-10985 ◽  
Author(s):  
B. G. Borghuis ◽  
J. S. Marvin ◽  
L. L. Looger ◽  
J. B. Demb
Keyword(s):  
Bipolar Cell ◽  
Glutamate Release ◽  
Mouse Retina ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals In vivo two-photon imaging of the mouse retina

2013 ◽  
Vol 4 (8) ◽  
pp. 1285 ◽  
Author(s):  
Robin Sharma ◽  
Lu Yin ◽  
Ying Geng ◽  
William H. Merigan ◽  
Grazyna Palczewska ◽  
...  
Keyword(s):  
Mouse Retina ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Adaptive optics via pupil ring segmentation improves spherical aberration correction for two-photon imaging of optically cleared tissues

2020 ◽  
Vol 28 (23) ◽  
pp. 34935
Author(s):  
Yufeng Gao ◽  
Lina Liu ◽  
Yixuan Yin ◽  
Jiuling Liao ◽  
Jia Yu ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Spherical Aberration ◽  
Aberration Correction ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Bayesian Hypothesis Testing And Experimental Design For Two-Photon Imaging Data

2018 ◽  
Author(s):  
Luke E. Rogerson ◽  
Zhijian Zhao ◽  
Katrin Franke ◽  
Philipp Berens ◽  
Thomas Euler
Keyword(s):  
Neural Activity ◽  
Ex Vivo ◽  
Gaussian Process Regression ◽  
Mouse Retina ◽  
Central Feature ◽  
Imaging Data ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
The Cost

AbstractVariability, stochastic or otherwise, is a central feature of neural circuits. Yet the means by which variation and uncertainty are derived from noisy observations of neural activity is often unprincipled, with too much weight placed on numerical convenience at the cost of statistical rigour. For two-photon imaging data, composed of fundamentally probabilistic streams of photon detections, the problem is particularly acute. Here, we present a complete statistical pipeline for the inference and analysis of neural activity using Gaussian Process Regression, applied to two-photon recordings of light-driven activity in ex vivo mouse retina. We demonstrate the flexibility and extensibility of these models, considering cases with non-stationary statistics, driven by complex parametric stimuli, in signal discrimination, hierarchical clustering and inference tasks. Sparse approximation methods allow these models to be fitted rapidly, permitting them to actively guiding the design of light stimulation in the midst of ongoing two-photon experiments.

Progress on Bimodal Adaptive Optics OCT and Two-Photon Imaging

2021 ◽  
Author(s):  
William Newberry ◽  
Laura Vargas ◽  
Marinko V. Sarunic
Keyword(s):  
Adaptive Optics ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals In vivo two-photon imaging of the mouse retina

2012 ◽  
Vol 12 (14) ◽  
pp. 51-51 ◽  
Author(s):  
R. Sharma ◽  
L. Yin ◽  
Y. Geng ◽  
W. H. Merigan ◽  
D. R. Williams ◽  
...  
Keyword(s):  
Mouse Retina ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging
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