scholarly journals Jointly super-resolved and optically sectioned Bayesian reconstruction method for structured illumination microscopy

2019 ◽  
Vol 27 (23) ◽  
pp. 33251 ◽  
Author(s):  
Yann Lai-Tim ◽  
Laurent M. Mugnier ◽  
François Orieux ◽  
Roberto Baena-Gallé ◽  
Michel Paques ◽  
...  
Keyword(s):  
Reconstruction Method ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Bayesian Reconstruction

scholarly journals The Influence of Noise in the Super-Resolution Reconstruction of Structured Illumination Microscopy

2021 ◽  
Vol 2112 (1) ◽  
pp. 012012
Author(s):  
Jinxi Bai ◽  
Zhendong Shi ◽  
Hua Ma ◽  
Lijia Liu ◽  
Lin Zhang
Keyword(s):  
Frequency Spectrum ◽  
Low Frequency ◽  
Super Resolution ◽  
Reconstruction Method ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Frequency Information ◽  
Image Reconstruction Method ◽  
Path Structure ◽  
Influence Of Noise

Abstract As one of the mainstream super-resolution imaging technologies, structured illumination microscopy (SIM) is popular for its fast imaging speed and simple optical path structure. Spectrum separation is a key step in the reconstruction of super-resolution images. However, in the process of imaging, the unavoidable noise will seriously affect the accuracy of frequency spectrum separation. This paper carries out a simulation study on the influence of noise in the process of frequency spectrum separation. The results show that although noise can cause distortion of low-frequency information in frequency spectrum separation results, it has little influence on high-frequency information. Therefore, a super-resolution image reconstruction method is proposed to effectively suppress the influence of noise. Both simulation and experimental results are shown the method can suppress the influence of noise without losing the details of super-resolution.

Structured illumination microscopy and correlative microscopy to study autophagy

Methods ◽  
2015 ◽  
Vol 75 ◽  
pp. 61-68 ◽  
Author(s):  
Laure-Anne Ligeon ◽  
Nicolas Barois ◽  
Elisabeth Werkmeister ◽  
Antonino Bongiovanni ◽  
Frank Lafont
Keyword(s):  
Structured Illumination ◽  
Correlative Microscopy ◽  
Structured Illumination Microscopy

Cost-Effective Live Cell Structured Illumination Microscopy with Video-Rate Imaging

ACS Photonics ◽  
2021 ◽  
Author(s):  
Alice Sandmeyer ◽  
Mario Lachetta ◽  
Hauke Sandmeyer ◽  
Wolfgang Hübner ◽  
Thomas Huser ◽  
...  
Keyword(s):  
Cost Effective ◽  
Live Cell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy

scholarly journals Growth factor dependent changes in nanoscale architecture of focal adhesions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karin Legerstee ◽  
Tsion E. Abraham ◽  
Wiggert A. van Cappellen ◽  
Alex L. Nigg ◽  
Johan A. Slotman ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Intermediate Layer ◽  
Cell Movement ◽  
Focal Adhesions ◽  
Bottom Layer ◽  
Vertical Axis ◽  
Longitudinal Distribution ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Mediate Cell

AbstractFocal adhesions (FAs) are flat elongated structures that mediate cell migration and link the cytoskeleton to the extracellular matrix. Along the vertical axis FAs were shown to be composed of three layers. We used structured illumination microscopy to examine the longitudinal distribution of four hallmark FA proteins, which we also used as markers for these layers. At the FA ends pointing towards the adherent membrane edge (heads), bottom layer protein paxillin protruded, while at the opposite ends (tails) intermediate layer protein vinculin and top layer proteins zyxin and VASP extended further. At the tail tips, only intermediate layer protein vinculin protruded. Importantly, head and tail compositions were altered during HGF-induced scattering with paxillin heads being shorter and zyxin tails longer. Additionally, FAs at protruding or retracting membrane edges had longer paxillin heads than FAs at static edges. These data suggest that redistribution of FA-proteins with respect to each other along FAs is involved in cell movement.

scholarly journals Film Thickness-Profile Measurement Using Iterative Peak Separation Structured Illumination Microscopy

2021 ◽  
Vol 11 (7) ◽  
pp. 3023
Author(s):  
Kejun Yang ◽  
Chenhaolei Han ◽  
Jinhua Feng ◽  
Yan Tang ◽  
Zhongye Xie ◽  
...  
Keyword(s):  
Modulation Depth ◽  
Thickness Distribution ◽  
Detection Threshold ◽  
Profile Measurement ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Separation Algorithm ◽  
Peak Separation ◽  
Lower Detection ◽  
Depth Response

The surface and thickness distribution measurement for transparent film is of interest for electronics and packaging materials. Structured illumination microscopy (SIM) is a prospective technique for measuring film due to its high accuracy and efficiency. However, when the distance between adjacent layers becomes close, the peaks of the modulation depth response (MDR) start to overlap and interfere with the peak extraction, which restricts SIM development in the field of film measurement. In this paper, an iterative peak separation algorithm is creatively applied in the SIM-based technique, providing a precise peak identification even as the MDR peaks overlap and bend into one. Compared with the traditional method, the proposed method has a lower detection threshold for thickness. The experiments and theoretical analysis are elaborated to demonstrate the feasibility of the mentioned method.

scholarly journals Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Liliana Barbieri ◽  
Huw Colin-York ◽  
Kseniya Korobchevskaya ◽  
Di Li ◽  
Deanna L. Wolfson ◽  
...  
Keyword(s):  
Resolution Enhancement ◽  
Traction Force ◽  
Super Resolution ◽  
Traction Force Microscopy ◽  
Two Dimensional ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Force Microscopy ◽  
Health And Disease ◽  
Spatio Temporal

AbstractQuantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

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