scholarly journals On-chip optical trapping of extracellular vesicles using box-shaped composite SiO2-Si3N4 waveguides

2018 ◽  
Vol 26 (21) ◽  
pp. 26985 ◽  
Author(s):  
Gyllion Brian Loozen ◽  
Jacob Caro
Keyword(s):  
Extracellular Vesicles ◽  
Optical Trapping ◽  
On Chip

An integrated microfluidic system for on-chip enrichment and quantification of circulating extracellular vesicles from whole blood

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3305-3315 ◽  
Author(s):  
Yi-Sin Chen ◽  
Yu-Dong Ma ◽  
Chihchen Chen ◽  
Shu-Chu Shiesh ◽  
Gwo-Bin Lee
Keyword(s):  
Extracellular Vesicles ◽  
Immunosorbent Assay ◽  
Whole Blood ◽  
Magnetic Beads ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Whole Blood ◽  
On Chip ◽  
Chip Enrichment

An integrated microfluidic system was developed for extracellular vesicle (EV) enrichment and quantification by using anti-CD63-coated magnetic beads and an on-chip enzyme-linked immunosorbent assay in human whole blood.

scholarly journals On-chip optical trapping and Raman spectroscopy using a TripleX dual-waveguide trap

2014 ◽  
Vol 22 (25) ◽  
pp. 30528 ◽  
Author(s):  
Martijn Boerkamp ◽  
Thijs van Leest ◽  
Jeroen Heldens ◽  
Arne Leinse ◽  
Marcel Hoekman ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Trapping ◽  
On Chip

On-chip optical trapping for atomic applications

2014 ◽  
Author(s):  
Maximillian A. Perez ◽  
Evan Salim ◽  
Daniel Farkas ◽  
Janet Duggan ◽  
Megan Ivory ◽  
...  
Keyword(s):  
Optical Trapping ◽  
On Chip

scholarly journals Rapid Detection and Trapping of Extracellular Vesicles by Electrokinetic Concentration for Liquid Biopsy on Chip

Micromachines ◽  
2018 ◽  
Vol 9 (6) ◽  
pp. 306 ◽  
Author(s):  
Lucia Cheung ◽  
Sarah Sahloul ◽  
Ajymurat Orozaliev ◽  
Yong-Ak Song
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Rapid Detection ◽  
On Chip

scholarly journals Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis

2020 ◽  
Author(s):  
Alessandro Gori ◽  
Alessandro Romanato ◽  
Greta Bergamaschi ◽  
Alessandro Strada ◽  
Paola Gagni ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Lipid Membrane ◽  
Binding Capacity ◽  
Microarray Platform ◽  
Label Free ◽  
New Class ◽  
Associated Proteins ◽  
Packing Defects ◽  
Binding Peptides ◽  
On Chip

Small extracellular vesicles (EVs) present fairly distinctive lipid membrane features in the extracellular environment. These include high curvature, lipid packing defects and a relative abundance in lipids such as phosphatidylserine and ceramide. EVs membrane could be then considered as a "universal" marker, alternative or complementary to traditional characteristic surface-associated proteins. Here we introduce the use of membrane sensing peptides as new, highly efficient ligands for EVs capturing onto bioanalytical chips to directly integrate EVs capturing and analysis on a microarray platform, even using serum without pre-isolation steps. EVs were analyzed by label-free, single particle counting and by fluorescence co-localization immune-staining with labelled anti-CD9/anti-CD63/anti-CD81 antibodies. Peptides performed as selective yet general EVs baits and showed a binding capacity higher than anti-tetraspanins antibodies. Insights into surface chemistry for optimal peptide performance are also discussed, as capturing efficiency is strictly bound to probes surface orientation and multivalency effects. We anticipate that this new class of ligands, also due to the versatility and limited costs of synthetic peptides, may greatly enrich the molecular toolbox for EVs analysis.

Nanofiltration of extracellular vesicles from human plasma & their on-chip qualification and quantification with a NanoBioAnalytical platform

Meta Gene ◽  
2018 ◽  
Vol 17 ◽  
pp. S7
Author(s):  
Obeid Sameh ◽  
Benoît Le Roy de Boiseaumarie ◽  
Celine Elie-Caille ◽  
Wilfrid Boireau ◽  
Ming Li Chou ◽  
...  
Keyword(s):  
Human Plasma ◽  
Extracellular Vesicles ◽  
On Chip

Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis

2019 ◽  
Author(s):  
Alessandro Gori ◽  
Alessandro Romanato ◽  
Greta Bergamaschi ◽  
Alessandro Strada ◽  
Paola Gagni ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Lipid Membrane ◽  
Binding Capacity ◽  
Microarray Platform ◽  
Label Free ◽  
New Class ◽  
Associated Proteins ◽  
Packing Defects ◽  
Binding Peptides ◽  
On Chip

Small extracellular vesicles (EVs) present fairly distinctive lipid membrane features in the extracellular environment. These include high curvature, lipid packing defects and a relative abundance in lipids such as phosphatidylserine and ceramide. EVs membrane could be then considered as a "universal" marker, alternative or complementary to traditional characteristic surface-associated proteins. Here we introduce the use of membrane sensing peptides as new, highly efficient ligands for EVs capturing onto bioanalytical chips to directly integrate EVs capturing and analysis on a microarray platform, even using serum without pre-isolation steps. EVs were analyzed by label-free, single particle counting and by fluorescence co-localization immune-staining with labelled anti-CD9/anti-CD63/anti-CD81 antibodies. Peptides performed as selective yet general EVs baits and showed a binding capacity higher than anti-tetraspanins antibodies. Insights into surface chemistry for optimal peptide performance are also discussed, as capturing efficiency is strictly bound to probes surface orientation and multivalency effects. We anticipate that this new class of ligands, also due to the versatility and limited costs of synthetic peptides, may greatly enrich the molecular toolbox for EVs analysis.

Analysis of single-cell differences by use of an on-chip microculture system and optical trapping

2001 ◽  
Vol 371 (2) ◽  
pp. 276-281 ◽  
Author(s):  
Yuichi Wakamoto ◽  
Ippei Inoue ◽  
Hiroyuki Moriguchi ◽  
Kenji Yasuda
Keyword(s):  
Single Cell ◽  
Optical Trapping ◽  
On Chip

scholarly journals On-Chip Immunoelectrophoresis of Extracellular Vesicles Released from Human Breast Cancer Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0123603 ◽  
Author(s):  
Takanori Akagi ◽  
Kei Kato ◽  
Masashi Kobayashi ◽  
Nobuyoshi Kosaka ◽  
Takahiro Ochiya ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
On Chip

On-chip supercontinuum optical trapping and resonance excitation of microspheres

2010 ◽  
Vol 35 (10) ◽  
pp. 1626 ◽  
Author(s):  
Arthur Nitkowski ◽  
Alexander Gondarenko ◽  
Michal Lipson
Keyword(s):  
Optical Trapping ◽  
Resonance Excitation ◽  
On Chip
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