scholarly journals Improving spatial resolution of confocal Raman microscopy by super-resolution image restoration

2016 ◽  
Vol 24 (10) ◽  
pp. 10767 ◽  
Author(s):  
Han Cui ◽  
Weiqian Zhao ◽  
Yun Wang ◽  
Ying Fan ◽  
Lirong Qiu ◽  
...  
2018 ◽  
Vol 91 (1) ◽  
pp. 1049-1055 ◽  
Author(s):  
Carol Korzeniewski ◽  
Jay P. Kitt ◽  
Saheed Bukola ◽  
Stephen E. Creager ◽  
Shelley D. Minteer ◽  
...  

2008 ◽  
Vol 14 (S2) ◽  
pp. 782-783
Author(s):  
M Kalbac ◽  
E Lee ◽  
A Zoubir ◽  
A Whitley

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008


2017 ◽  
Vol 100 ◽  
pp. 119-128 ◽  
Author(s):  
M. Torres-Carrasco ◽  
A. del Campo ◽  
M.A. de la Rubia ◽  
E. Reyes ◽  
A. Moragues ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4222
Author(s):  
Richard Krombholz ◽  
Dominique Lunter

In the development of dermal drug formulations and cosmetics, understanding the penetration properties of the active ingredients is crucial. Given that widespread methods, including tape stripping, lack in spatial resolution, while being time- and labour-intensive, Confocal Raman Microscopy is a promising alternative. In optimizing topically applied formulations, or the development of generic formulations, comparative in-situ measurements have a huge potential of saving time and resources. In this work, we show our approach to in-situ skin penetration analysis by confocal Raman Microscopy. To analyse feasibility of the approach, we used caffeine solutions as model vehicles and tested the effectiveness of 1,2-pentanediol as a penetration enhancer for delivery to the skin.


1993 ◽  
Vol 47 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Anurag Govil ◽  
David M. Pallister ◽  
Michael D. Morris

We describe an iterative image restoration technique which functions as digital confocal microscopy for Raman images. We deconvolute the lateral and axial components of the microscope point spread function from a series of optical sections, to generate a stack of well-resolved Raman images which describe the three-dimensional topology of a sample. The technique provides an alternative to confocal microscopy for three-dimensional microscopic Raman imaging.


Author(s):  
R. S. Hansen ◽  
D. W. Waldram ◽  
T. Q. Thai ◽  
R. B. Berke

Abstract Background High-resolution Digital Image Correlation (DIC) measurements have previously been produced by stitching of neighboring images, which often requires short working distances. Separately, the image processing community has developed super resolution (SR) imaging techniques, which improve resolution by combining multiple overlapping images. Objective This work investigates the novel pairing of super resolution with digital image correlation, as an alternative method to produce high-resolution full-field strain measurements. Methods First, an image reconstruction test is performed, comparing the ability of three previously published SR algorithms to replicate a high-resolution image. Second, an applied translation is compared against DIC measurement using both low- and super-resolution images. Third, a ring sample is mechanically deformed and DIC strain measurements from low- and super-resolution images are compared. Results SR measurements show improvements compared to low-resolution images, although they do not perfectly replicate the high-resolution image. SR-DIC demonstrates reduced error and improved confidence in measuring rigid body translation when compared to low resolution alternatives, and it also shows improvement in spatial resolution for strain measurements of ring deformation. Conclusions Super resolution imaging can be effectively paired with Digital Image Correlation, offering improved spatial resolution, reduced error, and increased measurement confidence.


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