Improving and Understanding Three Dimensional Spatial Resolution in a Confocal Raman Microscopy and Raman Hyperspectral Imaging I

2010 ◽  
Author(s):  
Eunah Lee ◽  
Bernard Roussel ◽  
Emmanuel Froigneux ◽  
Fran Adar ◽  
Sergey Mamedov ◽  
...  
2009 ◽  
Vol 15 (S2) ◽  
pp. 1142-1143
Author(s):  
E Lee ◽  
J Shaver ◽  
S Kock ◽  
F Adar ◽  
A Whitley

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009


2018 ◽  
Vol 91 (1) ◽  
pp. 1049-1055 ◽  
Author(s):  
Carol Korzeniewski ◽  
Jay P. Kitt ◽  
Saheed Bukola ◽  
Stephen E. Creager ◽  
Shelley D. Minteer ◽  
...  

2008 ◽  
Vol 14 (S2) ◽  
pp. 782-783
Author(s):  
M Kalbac ◽  
E Lee ◽  
A Zoubir ◽  
A Whitley

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008


2016 ◽  
Vol 24 (10) ◽  
pp. 10767 ◽  
Author(s):  
Han Cui ◽  
Weiqian Zhao ◽  
Yun Wang ◽  
Ying Fan ◽  
Lirong Qiu ◽  
...  

2017 ◽  
Vol 100 ◽  
pp. 119-128 ◽  
Author(s):  
M. Torres-Carrasco ◽  
A. del Campo ◽  
M.A. de la Rubia ◽  
E. Reyes ◽  
A. Moragues ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4222
Author(s):  
Richard Krombholz ◽  
Dominique Lunter

In the development of dermal drug formulations and cosmetics, understanding the penetration properties of the active ingredients is crucial. Given that widespread methods, including tape stripping, lack in spatial resolution, while being time- and labour-intensive, Confocal Raman Microscopy is a promising alternative. In optimizing topically applied formulations, or the development of generic formulations, comparative in-situ measurements have a huge potential of saving time and resources. In this work, we show our approach to in-situ skin penetration analysis by confocal Raman Microscopy. To analyse feasibility of the approach, we used caffeine solutions as model vehicles and tested the effectiveness of 1,2-pentanediol as a penetration enhancer for delivery to the skin.


1993 ◽  
Vol 47 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Anurag Govil ◽  
David M. Pallister ◽  
Michael D. Morris

We describe an iterative image restoration technique which functions as digital confocal microscopy for Raman images. We deconvolute the lateral and axial components of the microscope point spread function from a series of optical sections, to generate a stack of well-resolved Raman images which describe the three-dimensional topology of a sample. The technique provides an alternative to confocal microscopy for three-dimensional microscopic Raman imaging.


2012 ◽  
Vol 9 (7) ◽  
pp. 8273-8306 ◽  
Author(s):  
M. Wall ◽  
G. Nehrke

Abstract. Confocal Raman microscopy mapping was used to investigate the organic matrix distribution within the skeleton of the coral Porites lutea. Two types of growth lines could be identified: one corresponds to the well-known incremental growth layers, whereas the second type of growth lines showed an elemental composition that differed from the incremental growth layers. The position and shape of the latter growth lines resemble either denticle finger-like structures (most likely traces of former spines) or former skeletal surfaces. We hypothesize that these lines are involved in the three-dimensional arrangement of skeletal elements and represent the outer skeletal surface before another growth cycle of elongation, infilling and thickening of skeletal components continues. We show that high spatial resolution mapping can significantly improve our understanding of skeletal growth patterns in coral skeletons.


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