scholarly journals Nanoscale topography and spatial light modulator characterization using wide-field quantitative phase imaging

2014 ◽  
Vol 22 (3) ◽  
pp. 3432 ◽  
Author(s):  
Gannavarpu Rajshekhar ◽  
Basanta Bhaduri ◽  
Chris Edwards ◽  
Renjie Zhou ◽  
Lynford L. Goddard ◽  
...  
Keyword(s):  
Spatial Light Modulator ◽  
Wide Field ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Light Modulator ◽  
Quantitative Phase ◽  
Nanoscale Topography

High-resolution quantitative phase imaging based on a spatial light modulator and incremental binary random sampling

2020 ◽  
Vol 59 (20) ◽  
pp. 6148 ◽  
Author(s):  
Zhao Wang ◽  
Gong-Xiang Wei ◽  
Xiao-Lu Ge ◽  
Hui-Qiang Liu ◽  
Ben-Yi Wang
Keyword(s):  
High Resolution ◽  
Random Sampling ◽  
Spatial Light Modulator ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Light Modulator ◽  
Quantitative Phase

Quantitative phase imaging by wide field lensless digital holographic microscope

2015 ◽  
Author(s):  
A. Adinda-Ougba ◽  
N. Koukourakis ◽  
A. Essaidi ◽  
N. C. Ger­hardt ◽  
M. R. Hofmann
Keyword(s):  
Wide Field ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

scholarly journals Adaptive dynamic range shift (ADRIFT) quantitative phase imaging

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Keiichiro Toda ◽  
Miu Tamamitsu ◽  
Takuro Ideguchi
Keyword(s):  
Dynamic Range ◽  
Range Shift ◽  
Wide Field ◽  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Mid Infrared ◽  
Quantitative Phase ◽  
Adaptive Dynamic ◽  
Sensitivity Improvement

AbstractQuantitative phase imaging (QPI) with its high-contrast images of optical phase delay (OPD) maps is often used for label-free single-cell analysis. Contrary to other imaging methods, sensitivity improvement has not been intensively explored because conventional QPI is sensitive enough to observe the surface roughness of a substrate that restricts the minimum measurable OPD. However, emerging QPI techniques that utilize, for example, differential image analysis of consecutive temporal frames, such as mid-infrared photothermal QPI, mitigate the minimum OPD limit by decoupling the static OPD contribution and allow measurement of much smaller OPDs. Here, we propose and demonstrate supersensitive QPI with an expanded dynamic range. It is enabled by adaptive dynamic range shift through a combination of wavefront shaping and dark-field QPI techniques. As a proof-of-concept demonstration, we show dynamic range expansion (sensitivity improvement) of QPI by a factor of 6.6 and its utility in improving the sensitivity of mid-infrared photothermal QPI. This technique can also be applied for wide-field scattering imaging of dynamically changing nanoscale objects inside and outside a biological cell without losing global cellular morphological image information.

scholarly journals Fast photothermal spatial light modulation for quantitative phase imaging at the nanoscale

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Hadrien M. L. Robert ◽  
Kristýna Holanová ◽  
Łukasz Bujak ◽  
Milan Vala ◽  
Verena Henrichs ◽  
...  
Keyword(s):  
Super Resolution ◽  
Light Polarization ◽  
Spatial Light Modulators ◽  
Phase Imaging ◽  
Light Modulation ◽  
Quantitative Phase Imaging ◽  
Light Modulator ◽  
Microtubule Network ◽  
Quantitative Phase ◽  
Spatial Light Modulation

AbstractSpatial light modulators have become an essential tool for advanced microscopy, enabling breakthroughs in 3D, phase, and super-resolution imaging. However, continuous spatial-light modulation that is capable of capturing sub-millisecond microscopic motion without diffraction artifacts and polarization dependence is challenging. Here we present a photothermal spatial light modulator (PT-SLM) enabling fast phase imaging for nanoscopic 3D reconstruction. The PT-SLM can generate a step-like wavefront change, free of diffraction artifacts, with a high transmittance and a modulation efficiency independent of light polarization. We achieve a phase-shift > π and a response time as short as 70 µs with a theoretical limit in the sub microsecond range. We used the PT-SLM to perform quantitative phase imaging of sub-diffractional species to decipher the 3D nanoscopic displacement of microtubules and study the trajectory of a diffusive microtubule-associated protein, providing insights into the mechanism of protein navigation through a complex microtubule network.

Wavelength-scanning lensfree on-chip microscopy for wide-field pixel-super-resolved quantitative phase imaging

2021 ◽  
Author(s):  
Xuejuan Wu ◽  
Jiasong Sun ◽  
Jialin Zhang ◽  
Linpeng Lu ◽  
Rong Chen ◽  
...  
Keyword(s):  
Wide Field ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Wavelength Scanning ◽  
On Chip

scholarly journals Fast photothermal spatial light modulation for quantitative phase imaging at the nanoscale

2020 ◽  
Author(s):  
Hadrien Robert ◽  
Łukasz Bujak ◽  
Kristýna Holanová ◽  
Milan Vala ◽  
Piliarik Marek
Keyword(s):  
Super Resolution ◽  
Light Polarization ◽  
Spatial Light Modulators ◽  
Phase Imaging ◽  
Light Modulation ◽  
Quantitative Phase Imaging ◽  
Light Modulator ◽  
Quantitative Phase ◽  
Light Modulators ◽  
Spatial Light Modulation

Abstract Spatial light modulators have become an essential tool for advanced microscopy enabling breakthroughs in 3D, phase, or super-resolution imaging. However, continuous spatial-light modulation without diffraction artifacts, polarization dependence, and able to capture sub-ms microscopic motion is challenging. Here we present a photothermal spatial light modulator (PT-SLM) enabling the fast wavefront shaping free of diffraction artifacts, having a high transmissivity and modulation efficiency independent of light polarization. It is based on the microscopic heating of a thin layer of thermo-optic material confined between the photothermal heat-source and a transparent heatsink. We achieve a phase-shift > π with a response time as short as 70 µs with a theoretical limit in the sub-µs range. The combination of the PT-SLM with an interferometric scattering microscope (iSCAT) allowed us to perform quantitative phase imaging of sub-diffractional scatterers and decipher the 3D nanoscopic displacement of microtubules matching closely with control data from atomic force microscopy.

Sign in / Sign up

Export Citation Format

Share Document