scholarly journals Lateral resolution enhancement of laser scanning microscopy by a higher-order radially polarized mode beam

2011 ◽  
Vol 19 (17) ◽  
pp. 15947 ◽  
Author(s):  
Yuichi Kozawa ◽  
Terumasa Hibi ◽  
Aya Sato ◽  
Hibiki Horanai ◽  
Makoto Kurihara ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Resolution Enhancement ◽  
Higher Order ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Lateral Resolution ◽  
Mode Beam ◽  
Radially Polarized

scholarly journals Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection

2021 ◽  
Vol 11 (6) ◽  
pp. 2837
Author(s):  
Zhimin Zhang ◽  
Shaocong Liu ◽  
Minfei He ◽  
Yuran Huang ◽  
Cuifang Kuang ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Fluorescence Emission ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Lateral Resolution ◽  
Confocal Laser ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Three Dimension ◽  
Axial Resolution

Pixel reassignment image scanning microscopy (PRISM) is a useful tool to improve the resolution of confocal laser scanning microscopy (CLSM) only equipped with a detector array. However, while it can improve the lateral resolution, it has little effect on the axial resolution. Here, new microscopy has been proposed which combines three-dimension fluorescence emission difference microscopy (3D FED) with PRISM to further improve three-dimension resolution. We call this method three-dimension pixel reassignment fluorescence emission difference microscopy (3D-PRFED). Detailed theoretical analysis and simulation are presented in this paper. Additionally, the performance of lateral and axial resolution improvement of this method has been demonstrated by imaging 100 nm fluorescent beads and nuclear pore complexes samples. Experiment results show that this method in our system can improve lateral resolution by a factor of 1.85 and axial resolution by a factor of 1.48 compared with CLSM.

ACTIVATED SLUDGE FLOC CHARACTERIZATION BY CONFOCAL LASER SCANNING MICROSCOPY

2012 ◽  
Vol 11 (3) ◽  
pp. 669-674 ◽  
Author(s):  
Szabolcs Szilveszter ◽  
Botond Raduly ◽  
Szilard Bucs ◽  
Beata Abraham ◽  
Szabolcs Lanyi ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser
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