scholarly journals Spectral dependence of single molecule fluorescence enhancement

2007 ◽  
Vol 15 (21) ◽  
pp. 14266 ◽  
Author(s):  
Palash Bharadwaj ◽  
Lukas Novotny
Keyword(s):  
Single Molecule ◽  
Spectral Dependence ◽  
Fluorescence Enhancement ◽  
Single Molecule Fluorescence

scholarly journals Mixed metal zero-mode guides (ZMWs) for tunable fluorescence enhancement

2020 ◽  
Vol 2 (5) ◽  
pp. 1894-1903
Author(s):  
Abdullah Al Masud ◽  
W. Elliott Martin ◽  
Faruk H. Moonschi ◽  
So Min Park ◽  
Bernadeta R. Srijanto ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Zero Mode ◽  
Single Molecule Fluorescence ◽  
Mixed Metal

Mixed metal ZMWs tune single molecule fluorescence enhancement.

Combining gold nanoparticle antennas with single-molecule fluorescence resonance energy transfer (smFRET) to study DNA hairpin dynamics

Nanoscale ◽  
2018 ◽  
Vol 10 (14) ◽  
pp. 6611-6619 ◽  
Author(s):  
Jinyong Hu ◽  
Meiyan Wu ◽  
Li Jiang ◽  
Zhensheng Zhong ◽  
Zhangkai Zhou ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Gold Nanoparticle ◽  
Fluorescence Resonance Energy Transfer ◽  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Hairpin ◽  
Single Molecule Fluorescence ◽  
Fluorescence Resonance

Gold nanoparticle antennas as a promising platform not only for fluorescence enhancement but also for the studies of single-molecule kinetics.

scholarly journals Quantum Yield Limits for the Detection of Single-Molecule Fluorescence Enhancement by a Gold Nanorod

ACS Photonics ◽  
2020 ◽  
Vol 7 (9) ◽  
pp. 2498-2505
Author(s):  
Xuxing Lu ◽  
Gang Ye ◽  
Deep Punj ◽  
Ryan C. Chiechi ◽  
Michel Orrit
Keyword(s):  
Quantum Yield ◽  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Gold Nanorod ◽  
Single Molecule Fluorescence

Single-molecule fluorescence enhancement of a near-infrared dye by gold nanorods using DNA transient binding

2018 ◽  
Vol 20 (31) ◽  
pp. 20468-20475 ◽  
Author(s):  
Weichun Zhang ◽  
Martín Caldarola ◽  
Xuxing Lu ◽  
Biswajit Pradhan ◽  
Michel Orrit
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Gold Nanorods ◽  
Near Infrared ◽  
Fluorescence Enhancement ◽  
Plasmonic Nanostructures ◽  
New Materials ◽  
Single Molecule Fluorescence ◽  
Molecular Fluorescence ◽  
Near Infrared Dye

Fluorescence enhancement by plasmonic nanostructures enables the optical detection of single molecules with weak fluorescence, extending the scope of molecular fluorescence imaging to new materials and systems.

scholarly journals Single Molecule Fluorescence Imaging and Blinking

2006 ◽  
Vol 46 (3) ◽  
pp. 164-168
Author(s):  
Hiroaki YOKOTA ◽  
Tetsuichi WAZAWA ◽  
Yoshiharu ISHII
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Single Molecule Fluorescence

scholarly journals Single bacterial resolvases first exploit, then constrain intrinsic dynamics of the Holliday junction to direct recombination

2021 ◽  
Author(s):  
Sujay Ray ◽  
Nibedita Pal ◽  
Nils G Walter
Keyword(s):  
Cluster Analysis ◽  
Homologous Recombination ◽  
Single Molecule ◽  
Holliday Junction ◽  
Energetic Cost ◽  
Genomic Integrity ◽  
Dna Molecules ◽  
Single Molecule Fluorescence ◽  
And Cluster Analysis ◽  
Fluorescence Observation

Abstract Homologous recombination forms and resolves an entangled DNA Holliday Junction (HJ) crucial for achieving genetic reshuffling and genome repair. To maintain genomic integrity, specialized resolvase enzymes cleave the entangled DNA into two discrete DNA molecules. However, it is unclear how two similar stacking isomers are distinguished, and how a cognate sequence is found and recognized to achieve accurate recombination. We here use single-molecule fluorescence observation and cluster analysis to examine how prototypic bacterial resolvase RuvC singles out two of the four HJ strands and achieves sequence-specific cleavage. We find that RuvC first exploits, then constrains the dynamics of intrinsic HJ isomer exchange at a sampled branch position to direct cleavage toward the catalytically competent HJ conformation and sequence, thus controlling recombination output at minimal energetic cost. Our model of rapid DNA scanning followed by ‘snap-locking’ of a cognate sequence is strikingly consistent with the conformational proofreading of other DNA-modifying enzymes.

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