scholarly journals APPRAISAL "IN VITRO" OF ANTIMICROBIAL ACTIVITY PHOTODYNAMIC THERAPY ON STREPTOCOCCUS MUTANS OVER "IN SITU" DENTAL BIOFILM.

2012 ◽  
Author(s):  
V. H. Panhóca ◽  
F. E. Florez ◽  
N. Faria ◽  
A. N. S. Rastelli ◽  
J. M. G. Tanomaru ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Photodynamic Therapy ◽  
Streptococcus Mutans ◽  
Dental Biofilm

scholarly journals Antimicrobial Photodynamic Therapy and Dental Plaque: A Systematic Review of the Literature

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
G. C. Santin ◽  
D. S. B. Oliveira ◽  
R. Galo ◽  
M. C. Borsatto ◽  
S. A. M. Corona
Keyword(s):  
Systematic Review ◽  
Photodynamic Therapy ◽  
Data Extraction ◽  
Antimicrobial Photodynamic Therapy ◽  
Review Of The Literature ◽  
Eligibility Criteria ◽  
Dental Biofilm ◽  
Quality Assessments

Background. The aim of this study was to perform a systematic review of the literature on the efficacy of antimicrobial photodynamic therapy (PDTa) on cariogenic dental biofilm.Types of Studies Reviewed. Studiesin vivo,in vitro, andin situwere included. Articles that did not address PDTa, those that did not involve cariogenic biofilm, those that used microorganisms in the plankton phase, and reviews were excluded. Data extraction and quality assessments were performed independently by two raters using a scale.Results. Two hundred forty articles were retrieved; only seventeen of them met the eligibility criteria and were analyzed in the present review. Considerable variability was found regarding the methodologies and application protocols for antimicrobial PDTa. Two articles reported unfavorable results.Practical Implications. The present systematic review does not allow drawing any concrete conclusions regarding the efficacy of antimicrobial PDTa, although this method seems to be a promising option.

scholarly journals Function-adaptive clustered nanoparticles reverse Streptococcus mutans dental biofilm and maintain microbiota balance

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Fatemeh Ostadhossein ◽  
Parikshit Moitra ◽  
Esra Altun ◽  
Debapriya Dutta ◽  
Dinabandhu Sar ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Ex Vivo ◽  
Bacterial Species ◽  
Reactive Oxygen Species Generation ◽  
Biofilm Inhibition ◽  
16S Rrna Analysis ◽  
Dental Biofilm ◽  
Dental Plaques

AbstractDental plaques are biofilms that cause dental caries by demineralization with acidogenic bacteria. These bacteria reside inside a protective sheath which makes any curative treatment challenging. We propose an antibiotic-free strategy to disrupt the biofilm by engineered clustered carbon dot nanoparticles that function in the acidic environment of the biofilms. In vitro and ex vivo studies on the mature biofilms of Streptococcus mutans revealed >90% biofilm inhibition associated with the contact-mediated interaction of nanoparticles with the bacterial membrane, excessive reactive oxygen species generation, and DNA fragmentation. An in vivo examination showed that these nanoparticles could effectively suppress the growth of S. mutans. Importantly, 16S rRNA analysis of the dental microbiota showed that the diversity and richness of bacterial species did not substantially change with nanoparticle treatment. Overall, this study presents a safe and effective approach to decrease the dental biofilm formation without disrupting the ecological balance of the oral cavity.

scholarly journals The anti-adherence effect of Lippia sidoides Cham: extract against microorganisms of dental biofilm

2013 ◽  
Vol 15 (1) ◽  
pp. 41-46 ◽  
Author(s):  
A.C.L. Albuquerque ◽  
M.S.V. Pereira ◽  
D.F. Silva ◽  
L.F. Pereira ◽  
F.A.C. Viana ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Folk Medicine ◽  
Tooth Surface ◽  
Bacterial Strains ◽  
Lippia Sidoides ◽  
Dental Biofilm ◽  
Scientific Exploration ◽  
Infectious Origin ◽  
In Vitro Simulation

Most illnesses affecting the oral cavity are proven to have infectious origin. Several categories of chemical agents have been used in the chemical control of dental biofilm through strategies that aim at reducing bacterial adhesion and inhibiting the growth and the proliferation of microorganisms on the tooth surface. The use of plants in folk medicine and in Dentistry, as well as the spread of successful cases, has led to scientific exploration, resulting in chemical-pharmacological knowledge of thousands of plants. The present study aimed to evaluate the anti-adherence activity of Lippia sidoides Cham., comparing the results with those of 0.12% chlorhexidine by means of an in vitro simulation of dental biofilm. The studied bacterial strains were Streptococcus mutans, Streptococcus sanguinis and Lactobacillus casei, main responsible for the biofilm adherence. The studied extract was effective in inhibiting the adherence of Streptococcus mutans up to a concentration of 1:16, compared to Chlorhexidine. Lippia sidoides Cham extract showed anti-adherence effect on the major microorganisms responsible for dental biofilm consolidation.

Comparison of antibacterial efficacy of herbal extracts of aloe vera leaves and mushroom against streptococcus mutansand lactobacillus - an in vitro study

2017 ◽  
Vol 9 (1) ◽  
pp. 23-29
Author(s):  
Bijo Kurian
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Aloe Vera ◽  
In Vitro Study ◽  
Herbal Extracts ◽  
Zone Of Inhibition ◽  
Group Iii ◽  
Mushroom Extract ◽  
Group I

BACKGROUND AND OBJECTIVES: This study evaluated and compared the antimicrobial efficacy and minimal inhibitory concentration (MIC) of chlorhexidine 0.12% and extracts of aloe vera and mushroom against Streptococcus mutans and lactobacillus. MATERIALS & METHODOLOGY: The agar disc diffusion and broth micro dilution method was used to check the antimicrobial activity of 0.12% chlorhexidine and aqueous extracts of two medicinal plants. The test samples were divided as follows- Streptococcus mutans, group I: chlorhexidine, group II: Aloe vera and group III: Mushroom. Lactobacillus, Group I: chlorhexidine, group II: Aloe vera extract and group III: Mushroom extract. The zone of inhibition and MIC values were tabulated and the data was statistically analyzed using ANOVA and Bonferroni post- hoc tests. RESULTS: Chlorhexidine shows maximum antibacterial action against Streptococcus mutans and lactobacillus followed by mushroom extract and aloe veraextracts with the zone of inhibition of (20.3mm/ 24.13), (17.03/ 16.05), (14.09/14.26) respectively. Both the extracts had MIC value of 80µg/ ml and chlorhexidine was 40µg/ml. CONCLUSION: Within the limitations of this in- vitro study, it can be concluded that all the herbal extracts tested in this study demonstrated antibacterial activity against MS and lactobacillus. Chlorhexidine showed maximum antibacterial action against Streptococcus mutans and lactobacillus followed by mushroom extract and then aloe veraextracts with the zone of inhibition of (20.3mm/24.13), (17.03/ 16.05), (14.09/14.26) respectively. Herbal products have potent antimicrobial activity that can be looked at as an alternative to chlorhexidine. However, further in vitro and long-term in vivo studies are recommended to confirm and correlate the findings of this study clinically.

scholarly journals THE EFFECT OF CURCUMA XANTHORRHIZA ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)

2017 ◽  
Vol 10 (17) ◽  
pp. 30
Author(s):  
Royan Diana ◽  
Hedijanti Joenoes ◽  
Ariadna A Djais
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
Single Species ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Vitro Study ◽  
Dental Biofilm ◽  
Significant Difference ◽  
Curcuma Xanthorrhiza

Objective: This study aimed to compare the effect of Curcuma xanthrorrhiza ethanol extract to the viability of Streptococcus mutans and Aggregatibacter  actinomycetemcomitans using single- and dual-species biofilm at different phases of formation.Methods: Biofilm models were incubated for 4, 12, and 24 hrs, then exposed to the extract at a concentration of 0.525%.Results: The viability of the single-species S. mutans biofilm was low (p<0.05), and no significant difference (p>0.05) was found between singlespeciesA. actinomycetemcomitans and dual-species biofilm.Conclusions: Curcuma xanthorrhiza ethanol extract is more effective for decreasing the viability of single-species S. mutans biofilm.

scholarly journals Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

2020 ◽  
Vol 13 (06) ◽  
pp. 2050022
Author(s):  
Xiaoyue Liang ◽  
Zhaohui Zou ◽  
Zheng Zou ◽  
Changyi Li ◽  
Xiaoxi Dong ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Lactic Acid ◽  
Streptococcus Mutans ◽  
Antibacterial Effect ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Saline Control ◽  
Control Group ◽  
Plaque Biofilm

The main objective of this study is to evaluate the antibacterial effect of antibacterial photodynamic therapy (aPDT) on Streptococcus mutans (S. mutans) biofilm model in vitro. The selection of photosensitizers is the key step for the efficacy of photodynamic therapy (PDT). However, no studies have been conducted in the oral field to compare the functional characteristics and application effects of PDT mediated by various photosensitizers. In this research, the antibacterial effect of Methylene blue (MB)/650[Formula: see text]nm laser and Hematoporphyrin monomethyl ether (HMME)/532[Formula: see text]nm laser on S. mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT. The yield of lactic acid was analyzed by Colony forming unit (CFU) and spectrophotometry, and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy (CLSM) to evaluate the bactericidal effect on each group. Based on the results of CFU, the bacterial colonies formed by 30.4[Formula: see text]J/cm2 532[Formula: see text]nm MB-aPDT group and 30.4[Formula: see text]J/cm2 532[Formula: see text]nm HMME-aPDT group were significantly less than those in other groups, and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group. Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group. The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4[Formula: see text]J/cm2 aPDT. Therefore, aPDT suitable for energy measurement can kill S. mutans plaque biofilm, and MB-aPDT is better than HMME-aPDT.

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