Quasi-noise-free stimulated emission depletion microscopy imaging of thick samples using adaptive optics and block-matching 3D filtering

2019 ◽  
Author(s):  
Piotr Zdankowski ◽  
Maciej Trusiak ◽  
David McGloin ◽  
Jason R. Swedlow
Keyword(s):  
Adaptive Optics ◽  
Block Matching ◽  
Stimulated Emission ◽  
Microscopy Imaging ◽  
Stimulated Emission Depletion

scholarly journals Numerically Enhanced Stimulated Emission Depletion Microscopy with Adaptive Optics for Deep-Tissue Super-Resolved Imaging

ACS Nano ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 394-405 ◽  
Author(s):  
Piotr Zdańkowski ◽  
Maciej Trusiak ◽  
David McGloin ◽  
Jason R. Swedlow
Keyword(s):  
Adaptive Optics ◽  
Stimulated Emission ◽  
Deep Tissue ◽  
Stimulated Emission Depletion

scholarly journals Numerically enhanced adaptive optics-based 3D STED microscopy for deep-tissue super-resolved imaging

2019 ◽  
Author(s):  
Piotr Zdankowski ◽  
Maciej Trusiak ◽  
David McGloin ◽  
Jason R. Swedlow
Keyword(s):  
Adaptive Optics ◽  
Signal To Noise Ratio ◽  
Super Resolution ◽  
Thick Layer ◽  
Block Matching ◽  
Stimulated Emission ◽  
Optical Aberrations ◽  
Deep Tissue ◽  
Resolution Imaging ◽  
Induced Pluripotent

AbstractIn stimulated emission depletion (STED) nanoscopy, the major origin of decreased signal-to-noise ratio within images can be attributed to sample photobleaching and strong optical aberrations. This is due to STED utilising both a high power depletion laser (increasing risk of photodamage), while the depletion beam is very sensitive to sample-induced aberrations. Here we demonstrate a custom-built 3D STED microscope with automated aberration correction that is capable of 3D super-resolution imaging through thick, highly aberrating, tissue. We introduce and investigate image denoising by block-matching and collaborative filtering (BM3D) to numerically enhance fine object details otherwise mixed with noise. Numerical denoising provides an increase in the final effective resolution of the STED imaging of 31% using the well-established Fourier ring correlation metric. Experimental validation of the proposed method is achieved through super-resolved 3D imaging of axons in differentiated induced pluripotent stem cells growing under a 80µm thick layer of tissue with lateral and axial resolution of 256nm and 300nm, respectively.

scholarly journals Auto-aligning stimulated emission depletion microscope using adaptive optics

2013 ◽  
Vol 38 (11) ◽  
pp. 1860 ◽  
Author(s):  
Travis J. Gould ◽  
Emil B. Kromann ◽  
Daniel Burke ◽  
Martin J. Booth ◽  
Joerg Bewersdorf
Keyword(s):  
Adaptive Optics ◽  
Stimulated Emission ◽  
Stimulated Emission Depletion

scholarly journals Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy

2020 ◽  
Vol 28 (11) ◽  
pp. 16749 ◽  
Author(s):  
Jacopo Antonello ◽  
Aurélien Barbotin ◽  
Ee Zhuan Chong ◽  
Jens Rittscher ◽  
Martin J. Booth
Keyword(s):  
Adaptive Optics ◽  
Stimulated Emission ◽  
Multi Scale ◽  
Stimulated Emission Depletion

scholarly journals Stimulated Emission Depletion Microscopy with Color Centers in Hexagonal Boron Nitride

ACS Photonics ◽  
2021 ◽  
Author(s):  
Prince Khatri ◽  
Ralph Nicholas Edward Malein ◽  
Andrew J. Ramsay ◽  
Isaac J. Luxmoore
Keyword(s):  
Boron Nitride ◽  
Hexagonal Boron Nitride ◽  
Stimulated Emission ◽  
Color Centers ◽  
Stimulated Emission Depletion

scholarly journals Stimulated Emission Depletion Microscopy Resolves Individual Nitrogen Vacancy Centers in Diamond Nanocrystals

ACS Nano ◽  
2013 ◽  
Vol 7 (12) ◽  
pp. 10912-10919 ◽  
Author(s):  
Silvia Arroyo-Camejo ◽  
Marie-Pierre Adam ◽  
Mondher Besbes ◽  
Jean-Paul Hugonin ◽  
Vincent Jacques ◽  
...  
Keyword(s):  
Stimulated Emission ◽  
Nitrogen Vacancy ◽  
Nitrogen Vacancy Centers ◽  
Stimulated Emission Depletion
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