Evanescent Coupling of Fluorescence Emission into Waveguide Modes for Integrated Biochemical Sensors

2006 ◽  
Author(s):  
Lirong Wang ◽  
Nasser Peyghambarian ◽  
Sergio B. Mendes
2017 ◽  
Vol 9 (37) ◽  
pp. 31478-31487 ◽  
Author(s):  
Nhu Hoa Thi Tran ◽  
Jisoo Kim ◽  
Thang Bach Phan ◽  
Sungwon Khym ◽  
Heongkyu Ju

AIP Advances ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 075302
Author(s):  
Byungjun Kang ◽  
Kengo Motokura ◽  
Minoru Fujii ◽  
Dmitry V. Nesterenko ◽  
Zouheir Sekkat ◽  
...  

1990 ◽  
Vol 137 (2) ◽  
pp. 145 ◽  
Author(s):  
C.Y. Kim ◽  
S.D. Yu ◽  
R.F. Harrington ◽  
J.W. Ra ◽  
S.Y. Lee

1970 ◽  
Vol 117 (11) ◽  
pp. 2061 ◽  
Author(s):  
A.L. Cullen ◽  
O.J. Davies
Keyword(s):  

2017 ◽  
Author(s):  
Pedro M. R. Paulo ◽  
David Botequim ◽  
Agnieszka Jóskowiak ◽  
Sofia Martins ◽  
Duarte M. F. Prazeres ◽  
...  

<div> <div> <div> <p>We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon- enhanced fluorescence of this dye using dimers of 80 nm particles was measured at single molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and, in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from non-enhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. </p> </div> </div> </div>


2020 ◽  
Vol 17 (6) ◽  
pp. 472-478
Author(s):  
Wei-tao Gong ◽  
Wei-dong Qu ◽  
Guiling Ning

Two pyridinium amide-based receptors L1 and L2 with a small difference of H-bond position of the amide have been synthesized and characterized. Interestingly, they exhibited a huge difference in sensing towards AcO- and H2PO4 -, respectively. Receptor L1 was found to be ‘naked-eye’ selective for AcO- anions, while receptor L2 showed clear fluorescence enhancement selective to H2PO4 - anion. The recognition ability has been established by fluorescence emission, UV-vis spectra, and 1HNMR titration.


1982 ◽  
Vol 37 (3-4) ◽  
pp. 256-259 ◽  
Author(s):  
F. Schuler ◽  
P. Brandt ◽  
W. Wießner

Abstract An improved method for isolation of (photosystem II)-particles from Euglena gracilis, strain Z was established. PS II-particles isolated by ultrasonic treatment and following differential centrifugation show fluorescence emission and absorption spectra identical with in vivo properties of Euglena gracilis. These PS II-particles have only PS II-activity and contain CP a, the typical chlorophyll-protein-complex of PS II. No contamination of PS I-components are detectable.


2020 ◽  
Vol 74 (4) ◽  
pp. 439-451
Author(s):  
Philipp Holz ◽  
Christoph Pönisch ◽  
Albrecht Brandenburg

Imaging fluorescence spectroscopy proves to be a fast and sensitive method for measuring the thickness of thin coatings in the manufacturing industry. This encouraged us to systematically study, theoretically and experimentally, parameters that influence the fluorescence of thin layers. We analyzed the fluorescence signal as a function of the scattering and reflectance properties of the sample substrate. In addition, we investigated effects of the layer properties on fluorescence emission. A ray-tracing software is used to describe the influence of these parameters on the fluorescence emission of thin layers. Experiments using a custom-made system for imaging fluorescence analysis verify the simulations. This work shows a factor five variation of fluorescence intensity as a function of the reflectance of the sample substrate. Simulations show variations by a factor of up to eight for samples with different surface roughness. Results on tilted samples indicate a significant increase of the detected fluorescence signal, for fluorescent droplets on reflective substrates, if illuminated and coaxially observed at angles greater than 25°. These findings are of utmost relevance for all applications which utilize the fluorescence emission to quantify thin layers. These applications range from in-line lubricant monitoring in press plants to monitoring of functional coatings in medical technology and the detection of filmic contaminations.


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