3D fluorescence imaging of tumor cell spheroids

2011 ◽  
Author(s):  
Verena Richter ◽  
Thomas Bruns ◽  
Petra Weber ◽  
Wolfgang S.L. Strauβ ◽  
Rainer Wittig ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Fluorescence Imaging ◽  
Cell Spheroids

3D fluorescence imaging of tumor cell spheroids

2011 ◽  
Author(s):  
Verena Richter ◽  
Thomas Bruns ◽  
Petra Weber ◽  
Wolfgang S. L. Strauss ◽  
Rainer Wittig ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Fluorescence Imaging ◽  
Cell Spheroids

scholarly journals Predictive Models of Diffusive Nanoparticle Transport in 3-Dimensional Tumor Cell Spheroids

2013 ◽  
Vol 15 (3) ◽  
pp. 816-831 ◽  
Author(s):  
Yue Gao ◽  
Mingguang Li ◽  
Bin Chen ◽  
Zancong Shen ◽  
Peng Guo ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Predictive Models ◽  
Nanoparticle Transport ◽  
3 Dimensional ◽  
Cell Spheroids

Metabolism of alternative substrates and the bioenergetic status of EMT6 tumor cell spheroids

2000 ◽  
Vol 13 (6) ◽  
pp. 349-360 ◽  
Author(s):  
Janna P. Wehrle ◽  
Cheng E. Ng ◽  
Kathy A. McGovern ◽  
Nanci R. Aiken ◽  
Dikoma C. Shungu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Alternative Substrates ◽  
Cell Spheroids ◽  
Emt6 Tumor

Migration and internalization of cells and polystyrene microspheres in tumor cell spheroids

1982 ◽  
Vol 141 (1) ◽  
pp. 201-209 ◽  
Author(s):  
Mary J. Dorie ◽  
Robert F. Kallman ◽  
Diane F. Rapacchietta ◽  
Donna Van Antwerp ◽  
Yi Rong Huang
Keyword(s):  
Tumor Cell ◽  
Polystyrene Microspheres ◽  
Cell Spheroids

scholarly journals AQP3 Increases Intercellular Cohesion in NSCLC A549 Cell Spheroids through Exploratory Cell Protrusions

2021 ◽  
Vol 22 (8) ◽  
pp. 4287
Author(s):  
Sol Min ◽  
Chungyoul Choe ◽  
Sangho Roh
Keyword(s):  
Cell Surface ◽  
Tumor Cell ◽  
A549 Cell ◽  
Water Channel ◽  
3D Culture ◽  
Physical Contact ◽  
Culture Methods ◽  
Multicellular Aggregate ◽  
Cell Spheroids ◽  
Actomyosin Cytoskeleton

Tumor cell aggregation is critical for cell survival following the loss of extracellular matrix attachment and dissemination. However, the underlying mechanotransduction of clustering solitary tumor cells is poorly understood, especially in non-small cell lung cancers (NSCLC). Here, we examined whether cell surface protrusions played an important role in facilitating the physical contact between floating cells detached from a substrate. We employed poly-2-hydroxyethyl methacrylate-based 3D culture methods to mimic in vivo tumor cell cluster formation. The suprastructural analysis of human NSCLC A549 cell spheroids showed that finger-like protrusions clung together via the actin cytoskeleton. Time-lapse holotomography demonstrated that the finger-like protrusions of free-floating cells in 3D culture displayed exploratory coalescence. Global gene expression analysis demonstrated that the genes in the organic hydroxyl transport were particularly enriched in the A549 cell spheroids. Particularly, the knockdown of the water channel aquaporin 3 gene (AQP3) impaired multicellular aggregate formation in 3D culture through the rearrangement of the actomyosin cytoskeleton. Moreover, the cells with reduced levels of AQP3 decreased their transmigration. Overall, these data indicate that cell detachment-upregulated AQP3 contributes to cell surface protrusions through actomyosin cytoskeleton remodeling, causing the aggressive aggregation of free-floating cells dependent on the property of the substratum and collective metastasis.

scholarly journals Biologically excretable AIE nanoparticles wear tumor cell-derived “exosome caps” for efficient NIR-II fluorescence imaging-guided photothermal therapy

Nano Today ◽  
2021 ◽  
Vol 41 ◽  
pp. 101333
Author(s):  
Yirun Li ◽  
Xiaoxiao Fan ◽  
Yuanyuan Li ◽  
Liang Zhu ◽  
Runze Chen ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Fluorescence Imaging ◽  
Photothermal Therapy
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