Three-dimensional Fluorescence Imaging of Beads and Cancer Cells by Off-axis Incoherent Digital Holography

2018 ◽  
Author(s):  
Manoj Kumar ◽  
Xiangyu Quan ◽  
Yasuhiro Awatsuji ◽  
Osamu Matoba
Keyword(s):  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Digital Holography ◽  
Three Dimensional

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

scholarly journals pH and redox dual-sensitive drug delivery system constructed based on fluorescent carbon dots

RSC Advances ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 2656-2663
Author(s):  
Boye Zhang ◽  
Qianqian Duan ◽  
Yi Li ◽  
Jianming Wang ◽  
Wendong Zhang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Controlled Release ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Drug Delivery System ◽  
Delivery System ◽  
Carbon Dots ◽  
Ph Responsive ◽  
Charge Reversal ◽  
Fluorescent Carbon Dots

The system is pH-responsive and redox-controlled release. And the charge reversal and size transitions of the system can enhance the targeted ability. Moreover, the system can recognize the cancer cells by the fluorescence imaging.

scholarly journals Design, synthesis and application in biological imaging of a novel red fluorescent dye based on a rhodanine derivative

RSC Advances ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 160-163
Author(s):  
Zijing Li ◽  
Bin Huang ◽  
Yuan Wang ◽  
Wenbo Yuan ◽  
Yijing Wu ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Inhibitory Concentration ◽  
Fluorescent Dye ◽  
Biological Imaging ◽  
Design Synthesis ◽  
Red Emission ◽  
Rhodanine Derivative

2RDNTPA can be applied in fluorescence imaging of living cancer cells (HepG2) with red emission of 620 nm and negligible cytotoxicity with a half maximal inhibitory concentration much more than 100 μM.

Parallel Phase-Shifting Digital Holography Capable of Simultaneously Capturing Visible and Invisible Three-Dimensional Information

2010 ◽  
Vol 6 (10) ◽  
pp. 472-478 ◽  
Author(s):  
Takashi Kakue ◽  
Kenichi Ito ◽  
Tatsuki Tahara ◽  
Yasuhiro Awatsuji ◽  
Kenzo Nishio ◽  
...  
Keyword(s):  
Digital Holography ◽  
Three Dimensional ◽  
Phase Shifting

Androgen receptor: acting in the three-dimensional chromatin landscape of prostate cancer cells

2011 ◽  
Vol 5 (1) ◽  
Author(s):  
Harri Makkonen ◽  
Jorma J. Palvimo
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Cancer Cells ◽  
Binding Sites ◽  
Target Genes ◽  
Three Dimensional ◽  
Regulatory Elements ◽  
Gene Promoters ◽  
Loop Formation ◽  
Prostate Cancer Cells

AbstractAndrogen receptor (AR) acts as a hormone-controlled transcription factor that conveys the messages of both natural and synthetic androgens to the level of genes and gene programs. Defective AR signaling leads to a wide array of androgen insensitivity disorders, and deregulated AR function, in particular overexpression of AR, is involved in the growth and progression of prostate cancer. Classic models of AR action view AR-binding sites as upstream regulatory elements in gene promoters or their proximity. However, recent wider genomic screens indicate that AR target genes are commonly activated through very distal chromatin-binding sites. This highlights the importance of long-range chromatin regulation of transcription by the AR, shifting the focus from the linear gene models to three-dimensional models of AR target genes and gene programs. The capability of AR to regulate promoters from long distances in the chromatin is particularly important when evaluating the role of AR in the regulation of genes in malignant prostate cells that frequently show striking genomic aberrations, especially gene fusions. Therefore, in addition to the mechanisms of DNA loop formation between the enhancer bound ARs and the transcription apparatus at the target core promoter, the mechanisms insulating distally bound ARs from promiscuously making contacts and activating other than their normal target gene promoters are critical for proper physiological regulation and thus currently under intense investigation. This review discusses the current knowledge about the AR action in the context of gene aberrations and the three-dimensional chromatin landscape of prostate cancer cells.

scholarly journals Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull’s Eye-Type Plasmonic Chip

Sensors ◽  
2017 ◽  
Vol 17 (12) ◽  
pp. 2942 ◽  
Author(s):  
Shota Izumi ◽  
Shohei Yamamura ◽  
Naoko Hayashi ◽  
Mana Toma ◽  
Keiko Tawa
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Breast Cancer Cells ◽  
Dual Color ◽  
Bull's Eye
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