Quantitative Phase-Contrast Microscopy for Analysis of Live Cells by Using Lateral Shearing Approach in Digital Holography

2007 ◽  
Author(s):  
P. Ferraro ◽  
L. Miccio ◽  
S. Grilli ◽  
S. De Nicola ◽  
A. Finizio ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Digital Holography ◽  
Live Cells ◽  
Phase Contrast Microscopy ◽  
Quantitative Phase ◽  
Lateral Shearing ◽  
Contrast Microscopy

scholarly journals High-resolution quantitative phase-contrast microscopy by digital holography

2005 ◽  
Vol 13 (22) ◽  
pp. 8693 ◽  
Author(s):  
Christopher J. Mann ◽  
Lingfeng Yu ◽  
Chun-Min Lo ◽  
Myung K. Kim
Keyword(s):  
High Resolution ◽  
Phase Contrast ◽  
Digital Holography ◽  
Phase Contrast Microscopy ◽  
Quantitative Phase ◽  
Contrast Microscopy

scholarly journals Twin-beams digital holography for 3D tracking and quantitative phase-contrast microscopy in microfluidics

2011 ◽  
Vol 19 (25) ◽  
pp. 25833 ◽  
Author(s):  
Pasquale Memmolo ◽  
Andrea Finizio ◽  
Melania Paturzo ◽  
Lisa Miccio ◽  
Pietro Ferraro
Keyword(s):  
Phase Contrast ◽  
Digital Holography ◽  
Phase Contrast Microscopy ◽  
3D Tracking ◽  
Quantitative Phase ◽  
Contrast Microscopy

Quantitative phase contrast microscopy in turbid microfluidic channels by digital holography

2012 ◽  
Author(s):  
Vittorio Bianco ◽  
Melania Paturzo ◽  
Andrea Finizio ◽  
Pasquale Memmolo ◽  
Roberto Puglisi ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Digital Holography ◽  
Microfluidic Channels ◽  
Phase Contrast Microscopy ◽  
Quantitative Phase ◽  
Contrast Microscopy

Phase Contrast Microscopy with Digital Holography

1998 ◽  
Author(s):  
Etienne Cuche ◽  
Patrick Ruffieux ◽  
Patrick Poscio ◽  
Christian Depeursinge
Keyword(s):  
Phase Contrast ◽  
Digital Holography ◽  
Phase Contrast Microscopy ◽  
Contrast Microscopy

Phase-shifting Zernike phase contrast microscopy for quantitative phase measurement

2011 ◽  
Vol 36 (21) ◽  
pp. 4305 ◽  
Author(s):  
Peng Gao ◽  
Baoli Yao ◽  
Irina Harder ◽  
Norbert Lindlein ◽  
Francisco Jose Torcal-Milla
Keyword(s):  
Phase Contrast ◽  
Phase Measurement ◽  
Phase Shifting ◽  
Phase Contrast Microscopy ◽  
Quantitative Phase ◽  
Contrast Microscopy

Quantitative phase retrieval in X-ray Zernike phase contrast microscopy

2015 ◽  
Vol 22 (4) ◽  
pp. 1056-1061 ◽  
Author(s):  
Heng Chen ◽  
Zhili Wang ◽  
Kun Gao ◽  
Qiyue Hou ◽  
Dajiang Wang ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Phase Retrieval ◽  
Materials Science ◽  
Phase Contrast Microscopy ◽  
Contrast Imaging ◽  
X Ray ◽  
Quantitative Phase ◽  
Direct Light ◽  
Contrast Microscopy ◽  
Phase Variations

In recent years, increasing attention has been devoted to X-ray phase contrast imaging, since it can provide high-contrast images by using phase variations. Among the different existing techniques, Zernike phase contrast microscopy is one of the most popular phase-sensitive techniques for investigating the fine structure of the sample at high spatial resolution. In X-ray Zernike phase contrast microscopy, the image contrast is indeed a mixture of absorption and phase contrast. Therefore, this technique just provides qualitative information on the object, which makes the interpretation of the image difficult. In this contribution, an approach is proposed for quantitative phase retrieval in X-ray Zernike phase contrast microscopy. By shifting the phase of the direct light by π/2 and 3π/2, two images of the same object are measured successively. The phase information of the object can then be quantitatively retrieved by a proper combination of the measured images. Numerical experiments were carried out and the results confirmed the feasibility of the proposed method. It is expected that the proposed method will find widespread applications in biology, materials science and so on.

Sign in / Sign up

Export Citation Format

Share Document