scholarly journals All-optical imaging and manipulation of whole-brain neuronal activities in behaving larval zebrafish

2018 ◽  
Vol 9 (12) ◽  
pp. 6154 ◽  
Author(s):  
Zhen-Fei Jiao ◽  
Chun-Feng Shang ◽  
Yu-Fan Wang ◽  
Zhe Yang ◽  
Chen Yang ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
All Optical

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

Nature ◽  
2011 ◽  
Vol 478 (7368) ◽  
pp. 204-208 ◽  
Author(s):  
Tim Grotjohann ◽  
Ilaria Testa ◽  
Marcel Leutenegger ◽  
Hannes Bock ◽  
Nicolai T. Urban ◽  
...  
Keyword(s):  
Optical Imaging ◽  
All Optical

scholarly journals All-Optical Imaging of Gold Nanoparticle Geometry Using Super-Resolution Microscopy

2018 ◽  
Vol 122 (4) ◽  
pp. 2336-2342 ◽  
Author(s):  
Adam Taylor ◽  
René Verhoef ◽  
Michael Beuwer ◽  
Yuyang Wang ◽  
Peter Zijlstra
Keyword(s):  
Optical Imaging ◽  
Gold Nanoparticle ◽  
Super Resolution ◽  
All Optical ◽  
Super Resolution Microscopy

scholarly journals Review of micro-optical sectioning tomography (MOST): technology and applications for whole-brain optical imaging [Invited]

2019 ◽  
Vol 10 (8) ◽  
pp. 4075 ◽  
Author(s):  
Ting Zheng ◽  
Zhao Feng ◽  
Xiaojun Wang ◽  
Tao Jiang ◽  
Rui Jin ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Optical Sectioning ◽  
Whole Brain

scholarly journals Whole-brain calcium imaging during physiological vestibular stimulation in larval zebrafish

2018 ◽  
Author(s):  
Geoffrey Migault ◽  
Thomas Panier ◽  
Raphaël Candelier ◽  
Georges Debrégeas ◽  
Volker Bormuth
Keyword(s):  
Virtual Environments ◽  
Functional Imaging ◽  
Light Sheet ◽  
Vestibular Stimulation ◽  
Brain Response ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
In Vivo Functional Imaging ◽  
First Time

AbstractDuring in vivo functional imaging, animals are head-fixed and thus deprived from vestibular inputs, which severely hampers the design of naturalistic virtual environments. To overcome this limitation, we developed a miniaturized ultra-stable light-sheet microscope that can be dynamically rotated during imaging along with a head-restrained zebrafish larva. We demonstrate that this system enables whole-brain functional imaging at single-cell resolution under controlled vestibular stimulation. We recorded for the first time the dynamic whole-brain response of a vertebrate to physiological vestibular stimulation. This development largely expands the potential of virtual-reality systems to explore complex multisensory-motor integration in 3D.

scholarly journals A brainstem integrator for self-localization and positional homeostasis

2021 ◽  
Author(s):  
En Yang ◽  
Maarten F Zwart ◽  
Mikail Rubinov ◽  
Ben James ◽  
Ziqiang Wei ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Optic Flow ◽  
Inferior Olive ◽  
Brain Regions ◽  
Neural Pathway ◽  
Whole Brain ◽  
Water Currents ◽  
Larval Zebrafish ◽  
Integrate Optic ◽  
As If

To accurately track self-location, animals need to integrate their movements through space. In amniotes, representations of self-location have been found in regions such as the hippocampus. It is unknown whether more ancient brain regions contain such representations and by which pathways they may drive locomotion. Fish displaced by water currents must prevent uncontrolled drift to potentially dangerous areas. We found that larval zebrafish track such movements and can later swim back to their earlier location. Whole-brain functional imaging revealed the circuit enabling this process of positional homeostasis. Position-encoding brainstem neurons integrate optic flow, then bias future swimming to correct for past displacements by modulating inferior olive and cerebellar activity. Manipulation of position-encoding or olivary neurons abolished positional homeostasis or evoked behavior as if animals had experienced positional shifts. These results reveal a multiregional hindbrain circuit in vertebrates for optic flow integration, memory of self-location, and its neural pathway to behavior.

scholarly journals Whole-brain serial-section electron microscopy in larval zebrafish

2017 ◽  
Author(s):  
David Grant Colburn Hildebrand ◽  
Marcelo Cicconet ◽  
Russel Miguel Torres ◽  
Woohyuk Choi ◽  
Tran Minh Quan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Serial Section ◽  
Acquisition Time ◽  
Myelinated Axons ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
Section Electron ◽  
Serial Section Electron Microscopy ◽  
Section Electron Microscopy

Investigating the dense meshwork of wires and synapses that form neuronal circuits is possible with the high resolution of serial-section electron microscopy (ssEM)1. However, the imaging scale required to comprehensively reconstruct axons and dendrites is more than 10 orders of magnitude smaller than the spatial extents occupied by networks of interconnected neurons2—some of which span nearly the entire brain. The difficulties in generating and handling data for relatively large volumes at nanoscale resolution has thus restricted all studies in vertebrates to neuron fragments, thereby hindering investigations of complete circuits. These efforts were transformed by recent advances in computing, sample handling, and imaging techniques1, but examining entire brains at high resolution remains a challenge. Here we present ssEM data for a complete 5.5 days post-fertilisation larval zebrafish brain. Our approach utilizes multiple rounds of targeted imaging at different scales to reduce acquisition time and data management. The resulting dataset can be analysed to reconstruct neuronal processes, allowing us to, for example, survey all the myelinated axons (the projectome). Further, our reconstructions enabled us to investigate the precise projections of neurons and their contralateral counterparts. In particular, we observed that myelinated axons of reticulospinal and lateral line afferent neurons exhibit remarkable bilateral symmetry. Additionally, we found that fasciculated reticulospinal axons maintain the same neighbour relations throughout the extent of their projections. Furthermore, we use the dataset to set the stage for whole-brain comparisons of structure and function by co-registering functional reference atlases and in vivo two-photon fluorescence microscopy data from the same specimen. We provide the complete dataset and reconstructions as an open-access resource for neurobiologists and others interested in the ultrastructure of the larval zebrafish.

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