scholarly journals Review of micro-optical sectioning tomography (MOST): technology and applications for whole-brain optical imaging [Invited]

2019 ◽  
Vol 10 (8) ◽  
pp. 4075 ◽  
Author(s):  
Ting Zheng ◽  
Zhao Feng ◽  
Xiaojun Wang ◽  
Tao Jiang ◽  
Rui Jin ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Optical Sectioning ◽  
Whole Brain

scholarly journals All-optical imaging and manipulation of whole-brain neuronal activities in behaving larval zebrafish

2018 ◽  
Vol 9 (12) ◽  
pp. 6154 ◽  
Author(s):  
Zhen-Fei Jiao ◽  
Chun-Feng Shang ◽  
Yu-Fan Wang ◽  
Zhe Yang ◽  
Chen Yang ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
All Optical

scholarly journals Whole-Brain Three-Dimensional Profiling Reveals Brain Region Specific Axon Vulnerability in 5xFAD Mouse Model

2020 ◽  
Vol 14 ◽  
Author(s):  
Jianping Zhang ◽  
Ben Long ◽  
Anan Li ◽  
Qingtao Sun ◽  
Jiaojiao Tian ◽  
...  
Keyword(s):  
Mouse Model ◽  
Early Stage ◽  
Three Dimensional ◽  
Brain Regions ◽  
Pathological Feature ◽  
Optical Sectioning ◽  
Whole Brain ◽  
Early Symptom ◽  
Tomography System ◽  
5Xfad Mouse

Axonopathy is a pathological feature observed in both Alzheimer’s disease (AD) patients and animal models. However, identifying the temporal and regional progression of axonopathy during AD development remains elusive. Using the fluorescence micro-optical sectioning tomography system, we acquired whole-brain datasets in the early stage of 5xFAD/Thy1-GFP-M mice. We reported that among GFP labeled axons, GFP-positive axonopathy first formed in the lateral septal nucleus, subiculum, and medial mammillary nucleus. The axonopathy further increased in most brain regions during aging. However, most of the axonopathic varicosities disappeared significantly in the medial mammillary nucleus after 8 weeks old. Continuous three-dimensional datasets showed that axonopathy in the medial mammillary nucleus was mainly located on axons from hippocampal GFP-positive neurons. Using the rabies viral tracer in combination with immunohistochemistry, we found that axons in the medial mammillary nucleus from the subiculum were susceptible to lesions that prior to the occurrence of behavioral disorders. In conclusion, we created an early-stage spatiotemporal map of axonopathy in 5xFAD/Thy1-GFP-M mice and identified specific neural circuits which are vulnerable to axon lesions in an AD mouse model. These findings underline the importance of early interventions for AD, and may contribute to the understanding of its progression and its early symptom treatment.

Whole brain optical imaging

2015 ◽  
Author(s):  
Ludovico Silvestri ◽  
Anna Letizia Allegra Mascaro ◽  
Irene Costantini ◽  
Leonardo Sacconi ◽  
Francesco S. Pavone
Keyword(s):  
Optical Imaging ◽  
Whole Brain

scholarly journals Whole-brain imaging and characterization of Drosophila brains based on one-, two-, and three-photon excitations

2018 ◽  
Author(s):  
Kuo-Jen Hsu ◽  
Yen-Yin Lin ◽  
Ann-Shyn Chiang ◽  
Shi-Wei Chu
Keyword(s):  
High Speed ◽  
Optical Sectioning ◽  
Whole Brain ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Functional Connectome ◽  
Molecular Specificity ◽  
The Brain

AbstractTo study functional connectome, optical microscopy provides the advantages of in vivo observation, molecular specificity, high-speed acquisition, and sub-micrometer spatial resolution. Now, the most complete single-neuron-based anatomical connectome is built upon Drosophila; thus it will be a milestone to achieve whole-brain observation with sub-cellular resolution in living Drosophila. Surprisingly, two-photon microscopy cannot penetrate through the 200-μm-thick brain, due to the extraordinarily strong aberration/scattering from tracheae. Here we achieve whole-Drosophila-brain observation by degassing the brain or by using three-photon microscopy at 1300-nm, while only the latter provides in vivo feasibility, reduced aberration/scattering and exceptional optical sectioning capability. Furthermore, by comparing one-photon (488-nm), two-photon (920-nm), and three-photon (1300-nm) excitations in the brain, we not only demonstrate first quantitative reduction of both scattering and aberration in trachea-filled tissues, but unravel that the contribution of aberration exceeds scattering at long wavelengths. Our work paves the way toward constructing functional connectome in a living Drosophila.

scholarly journals Whole-Brain Reconstruction of Neurons in the Ventral Pallidum Reveals Diverse Projection Patterns

2021 ◽  
Vol 15 ◽  
Author(s):  
Qiru Feng ◽  
Sile An ◽  
Ruiyu Wang ◽  
Rui Lin ◽  
Anan Li ◽  
...  
Keyword(s):  
Single Neuron ◽  
Neural Circuit ◽  
Imaging System ◽  
Ventral Pallidum ◽  
Imaging Data ◽  
Subcortical Structures ◽  
Optical Sectioning ◽  
Whole Brain ◽  
Brain Imaging Data ◽  
Axonal Density

The ventral pallidum (VP) integrates reward signals to regulate cognitive, emotional, and motor processes associated with motivational salience. Previous studies have revealed that the VP projects axons to many cortical and subcortical structures. However, descriptions of the neuronal morphologies and projection patterns of the VP neurons at the single neuron level are lacking, thus hindering the understanding of the wiring diagram of the VP. In this study, we used recently developed progress in robust sparse labeling and fluorescence micro-optical sectioning tomography imaging system (fMOST) to label mediodorsal thalamus-projecting neurons in the VP and obtain high-resolution whole-brain imaging data. Based on these data, we reconstructed VP neurons and classified them into three types according to their fiber projection patterns. We systematically compared the axonal density in various downstream centers and analyzed the soma distribution and dendritic morphologies of the various subtypes at the single neuron level. Our study thus provides a detailed characterization of the morphological features of VP neurons, laying a foundation for exploring the neural circuit organization underlying the important behavioral functions of VP.

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