scholarly journals A compact photonic resonator absorption microscope for point of care digital resolution nucleic acid molecular diagnostics: publisher’s note

2021 ◽  
Author(s):  
Sarah J. Walker ◽  
Shreya Ghosh ◽  
Nantao Li ◽  
Yanyu Xiong ◽  
Young-gu Ju ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnostics ◽  
Point Of Care ◽  
Digital Resolution

scholarly journals A portable photonic resonator absorption microscope for point of care digital resolution nucleic acid molecular diagnostics

2021 ◽  
Author(s):  
Shreya Ghosh ◽  
Nantao Li ◽  
Yanyu Xiong ◽  
Young-gu Ju ◽  
Michael P. Rathslag ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnostics ◽  
Point Of Care ◽  
Digital Resolution

scholarly journals A glucose meter interface for point-of-care gene circuit-based diagnostics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Evan Amalfitano ◽  
Margot Karlikow ◽  
Masoud Norouzi ◽  
Katariina Jaenes ◽  
Seray Cicek ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Synthetic Biology ◽  
Molecular Diagnostics ◽  
Point Of Care ◽  
Low Cost ◽  
Gene Circuit ◽  
Reporter Systems ◽  
Glucose Meter ◽  
Pandemic Response ◽  
Glucose Output

AbstractRecent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2.

scholarly journals Nanomaterials for molecular signal amplification in electrochemical nucleic acid biosensing: recent advances and future prospects for point-of-care diagnostics

2020 ◽  
Vol 5 (1) ◽  
pp. 49-66 ◽  
Author(s):  
Léonard Bezinge ◽  
Akkapol Suea-Ngam ◽  
Andrew J. deMello ◽  
Chih-Jen Shih
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnostics ◽  
Signal Amplification ◽  
Point Of Care ◽  
Future Prospects ◽  
Electrochemical Biosensing ◽  
Recent Advances ◽  
Point Of Care Diagnostics ◽  
Molecular Signal

This account reviews the major amplification strategies utilizing nanomaterials in electrochemical biosensing for robust and sensitive molecular diagnostics.

Translating Nucleic Acid Amplification Assays to the Microscale: Lab on a Chip for Point-of-Care Molecular Diagnostics

2016 ◽  
Vol 12 (5) ◽  
pp. 386-396 ◽  
Author(s):  
Michael G. Mauk ◽  
Jinzhao Song ◽  
Yubing Tong ◽  
Haim H. Bau ◽  
Changchun Liu
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnostics ◽  
Point Of Care ◽  
Lab On A Chip ◽  
Nucleic Acid Amplification

scholarly journals Dynamic aqueous multiphase reaction system for simple, sensitive and quantitative one-pot CRISPR-Cas12a based molecular diagnosis

2020 ◽  
Author(s):  
Kun Yin ◽  
Xiong Ding ◽  
Ziyue Li ◽  
Hui Zhao ◽  
Kumarasen Cooper ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnosis ◽  
Molecular Diagnostics ◽  
Point Of Care ◽  
Sucrose Concentration ◽  
Reaction System ◽  
Fluorescent Detection ◽  
Quantitative Detection ◽  
One Pot ◽  
Multiphase Reaction

AbstractRecently, CRISPR-Cas technology has opened a new era of nucleic acid-based molecular diagnostics. However, current CRISPR-Cas-based nucleic acid biosensing has largely a lack of the quantitative detection ability and typically requires separate manual operations. Herein, we reported a dynamic aqueous multiphase reaction (DAMR) system for simple, sensitive and quantitative one-pot CRISPR-Cas12a based molecular diagnosis by taking advantage of density difference of sucrose concentration. In the DAMR system, recombinase polymerase amplification (RPA) and CRISPR-Cas12a derived fluorescent detection occurred in spatially separated but connected aqueous phases. Our DAMR system was utilized to quantitatively detect human papillomavirus (HPV) 16 and 18 DNAs with sensitivities of 10 and 100 copies within less than one hour. Multiplex detection of HPV16/18 in clinical human swab samples were successfully achieved in the DAMR system using 3D-printed microfluidic device. Furthermore, we demonstrated that target DNA in real human plasma samples can be directly amplified and detected in the DAMR system without complicated sample pre-treatment. As demonstrated, the DAMR system has shown great potential for development of next-generation point-of-care molecular diagnostics.

scholarly journals A portable, pressure driven, room temperature nucleic acid extraction and storage system for point of care molecular diagnostics

2013 ◽  
Vol 5 (13) ◽  
pp. 3177 ◽  
Author(s):  
Samantha Byrnes ◽  
Andy Fan ◽  
Jacob Trueb ◽  
Francis Jareczek ◽  
Mark Mazzochette ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnostics ◽  
Room Temperature ◽  
Point Of Care ◽  
Storage System ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
And Storage ◽  
Pressure Driven

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

scholarly journals Comparison of four commercial, automated antigen tests to detect SARS-CoV-2 variants of concern

2021 ◽  
Author(s):  
Andreas Osterman ◽  
Maximilian Iglhaut ◽  
Andreas Lehner ◽  
Patricia Späth ◽  
Marcel Stern ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Study Cohort ◽  
Operating Characteristics ◽  
Testing Strategies ◽  
Level 3 ◽  
Amino Acid Mutations ◽  
Antigen Tests ◽  
Roche Diagnostics ◽  
Analytical Sensitivities

AbstractA versatile portfolio of diagnostic tests is essential for the containment of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) pandemic. Besides nucleic acid-based test systems and point-of-care (POCT) antigen (Ag) tests, quantitative, laboratory-based nucleocapsid Ag tests for SARS-CoV-2 have recently been launched. Here, we evaluated four commercial Ag tests on automated platforms and one POCT to detect SARS-CoV-2. We evaluated PCR-positive (n = 107) and PCR-negative (n = 303) respiratory swabs from asymptomatic and symptomatic patients at the end of the second pandemic wave in Germany (February–March 2021) as well as clinical isolates EU1 (B.1.117), variant of concern (VOC) Alpha (B.1.1.7) or Beta (B.1.351), which had been expanded in a biosafety level 3 laboratory. The specificities of automated SARS-CoV-2 Ag tests ranged between 97.0 and 99.7% (Lumipulse G SARS-CoV-2 Ag (Fujirebio): 97.03%, Elecsys SARS-CoV-2 Ag (Roche Diagnostics): 97.69%; LIAISON® SARS-CoV-2 Ag (Diasorin) and SARS-CoV-2 Ag ELISA (Euroimmun): 99.67%). In this study cohort of hospitalized patients, the clinical sensitivities of tests were low, ranging from 17.76 to 52.34%, and analytical sensitivities ranged from 420,000 to 25,000,000 Geq/ml. In comparison, the detection limit of the Roche Rapid Ag Test (RAT) was 9,300,000 Geq/ml, detecting 23.58% of respiratory samples. Receiver-operating-characteristics (ROCs) and Youden’s index analyses were performed to further characterize the assays’ overall performance and determine optimal assay cutoffs for sensitivity and specificity. VOCs carrying up to four amino acid mutations in nucleocapsid were detected by all five assays with characteristics comparable to non-VOCs. In summary, automated, quantitative SARS-CoV-2 Ag tests show variable performance and are not necessarily superior to a standard POCT. The efficacy of any alternative testing strategies to complement nucleic acid-based assays must be carefully evaluated by independent laboratories prior to widespread implementation.

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