scholarly journals A portable, pressure driven, room temperature nucleic acid extraction and storage system for point of care molecular diagnostics

2013 ◽  
Vol 5 (13) ◽  
pp. 3177 ◽  
Author(s):  
Samantha Byrnes ◽  
Andy Fan ◽  
Jacob Trueb ◽  
Francis Jareczek ◽  
Mark Mazzochette ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnostics ◽  
Room Temperature ◽  
Point Of Care ◽  
Storage System ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
And Storage ◽  
Pressure Driven

Equipment-free nucleic acid extraction and amplification on a simple paper disc for point-of-care diagnosis of rotavirus A

2018 ◽  
Vol 1018 ◽  
pp. 78-85 ◽  
Author(s):  
Xin Ye ◽  
Jin Xu ◽  
Lijuan Lu ◽  
Xinxin Li ◽  
Xueen Fang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Rotavirus A ◽  
Paper Disc

scholarly journals A point of care platform based on microfluidic chip for nucleic acid extraction in less than 1 minute

2019 ◽  
Vol 13 (3) ◽  
pp. 034102 ◽  
Author(s):  
Jianzhong Zhang ◽  
Xiaosong Su ◽  
Jiasu Xu ◽  
Jin Wang ◽  
Juntian Zeng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Microfluidic Chip ◽  
Point Of Care ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Anja Gulliksen ◽  
Helen Keegan ◽  
Cara Martin ◽  
John O'Leary ◽  
Lars A. Solli ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Clinical Sample ◽  
Point Of Care ◽  
Fluorescent Detection ◽  
Clinical Samples ◽  
Microfluidic Channels ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Hpv E6

The paper presents the development of a “proof-of-principle” hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module. Nucleic acid extracts (n=28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%–85.7%. A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module. For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly. The modules of a “sample-in, answer-out” diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection.

scholarly journals Facile silicification of plastic surface for bioassays

2017 ◽  
Vol 53 (13) ◽  
pp. 2134-2137 ◽  
Author(s):  
Seonki Hong ◽  
Ki Soo Park ◽  
Ralph Weissleder ◽  
Cesar M. Castro ◽  
Hakho Lee
Keyword(s):  
Nucleic Acid ◽  
Surface Chemistry ◽  
Point Of Care ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Plastic Substrates ◽  
Biomimetic Surface ◽  
Plastic Surface

We report a biomimetic surface chemistry to modify plastic substrates for point-of-care nucleic acid extraction.

Design of Automatic Nucleic Acid Extraction Instrument Based on Magnetic Nanobeads

2012 ◽  
Vol 621 ◽  
pp. 313-316
Author(s):  
Yong Jie Dou ◽  
Jian Sheng Xu ◽  
Cong Liang Deng ◽  
Xiao Li Zhao ◽  
Leng Nie ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Room Temperature ◽  
Handling Time ◽  
Sample Volume ◽  
Sample Temperature ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Sample Handling ◽  
Sample Throughput ◽  
Magnetic Nanobeads

In this paper, an automatic nucleic acid extraction instrument is described. It consists of mechanical components, motor controlling module, temperature controlling module and computer. Also, a prototype has been designed and constructed. The maximum sample throughput of this prototype is 96, the sample volume is 300-1000ul, the maximum sample handling time is about 60min, the sample temperature controlling range is from room temperature to 95oC, and the accuracy of temperature controlling achieved is about ± 2oC.

scholarly journals Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Nasir Ali ◽  
Rita de Cássia Pontello Rampazzo ◽  
Alexandre Dias Tavares Costa ◽  
Marco Aurelio Krieger
Keyword(s):  
Nucleic Acid ◽  
Extraction Method ◽  
Point Of Care ◽  
Extraction Methods ◽  
Vital Role ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Advantages And Disadvantages ◽  
Point Of Care Diagnostics ◽  
Working Principle

Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx). POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at thepoint of careorpoint of need. Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx. In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems. At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx. We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems. We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.

Advances in Fully Automated Nucleic Acid Extraction System Based on Magnetic Nanobeads

2019 ◽  
Vol 11 (12) ◽  
pp. 1633-1643
Author(s):  
Zhu Chen ◽  
Changhu Xiao ◽  
Manling Tang ◽  
Yuyue Xu ◽  
Ziyu He ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Point Of Care ◽  
Low Cost ◽  
Future Trend ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Advantages And Disadvantages ◽  
Good Repeatability ◽  
Magnetic Nanobeads

Using magnetic nanobeads (MNBs) to extract nucleic acids is an efficient, inexpensive, easy automation, high throughput and good repeatability, instead of the traditional nucleic acid extraction (NAE) methods. Advances in fully automated MNBs-based nucleic acid extraction systems (MNAES) can push the frontiers of point-of-care testing (POCT) devices towards low-cost, automation, and enhanced accuracy molecular-level diagnostics. So, this paper introduces the pipettingbased MNAES with position of magnetic separation kit or tip, and based on magnetic bar MNAES with blending manner is shock or rotation. Further, advantages and disadvantages of various MNAES are compared. We envisage that the future trend in molecular diagnosis and monitoring will be facilitation, intelligent, miniaturization, and high throughput MNAES with sample-in-answer-out capability.

One-Step Release of DNA Rings From Polyoma Virions

1968 ◽  
Vol 26 ◽  
pp. 34-35
Author(s):  
C. Vasquez ◽  
A. K. Kleinschmidt
Keyword(s):  
Nucleic Acid ◽  
Room Temperature ◽  
Sodium Perchlorate ◽  
Length Distribution ◽  
Uranyl Acetate ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
One Step ◽  
Protein Monolayer ◽  
The One

A tightly packed regularly coiled DNA ring is assumed to be present inside the polyoma virion. Applying the “one-step” technique of nucleic acid extraction from virions while being adsorbed to a protein monolayer, we studied the configurational changes of the DNA.Intact polyoma virions were mixed with DFP-trypsin (100μg/ml) and spread onto a subphase of a mixture of urea and sodium perchlorate (6M and 2M, or 3M and 1M respectively), at pH 8.2 and room temperature. After spreading the film, aliquots were transferred to grids at different periods of time, and the DNA stained by acetonic uranyl acetate.The length distribution of the DNA molecules measured from electron micrographs is shown in Fig. 1. The number of twists present at different times after spreading were also counted. At 12 minutes, with 3M urea and 1M sodium perchlorate in the subphase, the DNA was found to be 85% supertwisted (Fig. 2); 12% was opened to rings, and 3% existed as linear filaments.

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