scholarly journals Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy

2021 ◽  
Author(s):  
Giuseppe de Vito ◽  
Lapo Turrini ◽  
Marie-Caroline Muellenbroich ◽  
pietro ricci ◽  
Giuseppe Sancataldo ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Zebrafish Larvae

scholarly journals Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish

2015 ◽  
Vol 6 (5) ◽  
pp. 1797 ◽  
Author(s):  
Zhe Yang ◽  
Li Mei ◽  
Fei Xia ◽  
Qingming Luo ◽  
Ling Fu ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Light Sheet Microscopy

Light-Sheet Microscopy for Whole-Brain Imaging

2018 ◽  
pp. 69-81
Author(s):  
Monika Pawłowska ◽  
Marzena Stefaniuk ◽  
Diana Legutko ◽  
Leszek Kaczmarek
Keyword(s):  
Brain Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Light Sheet Microscopy

High-fidelity functional and structural whole-brain imaging with Bessel-beam light-sheet microscopy (Conference Presentation)

2017 ◽  
Author(s):  
Marie Caroline Müllenbroich ◽  
Ludovico Silvestri ◽  
Lapo Turrini ◽  
Antonino Paolo Di Giovanna ◽  
Tommaso Alterini ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Bessel Beam ◽  
Light Sheet ◽  
High Fidelity ◽  
Whole Brain ◽  
Light Sheet Microscopy

Whole-brain functional imaging with two-photon light-sheet microscopy

2015 ◽  
Vol 12 (5) ◽  
pp. 379-380 ◽  
Author(s):  
Sébastien Wolf ◽  
Willy Supatto ◽  
Georges Debrégeas ◽  
Pierre Mahou ◽  
Sergei G Kruglik ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy

scholarly journals Two-photon light-sheet microscopy for high-speed whole-brain functional imaging of zebrafish neuronal physiology and pathology

2020 ◽  
Author(s):  
Giuseppe de Vito ◽  
Lapo Turrini ◽  
Chiara Fornetto ◽  
Pietro Ricci ◽  
Caroline Müllenbroich ◽  
...  
Keyword(s):  
Functional Imaging ◽  
High Speed ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Neuronal Physiology

scholarly journals Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging

2021 ◽  
Vol 15 ◽  
Author(s):  
Zhouzhou Zhang ◽  
Xiao Yao ◽  
Xinxin Yin ◽  
Zhangcan Ding ◽  
Tianyi Huang ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Light Sheet ◽  
Dendritic Morphology ◽  
Mammalian Brain ◽  
Wide Field ◽  
Whole Brain ◽  
Multi Scale ◽  
Light Sheet Microscopy ◽  
Constant Quality

Whole-brain imaging has become an increasingly important approach to investigate neural structures, such as somata distribution, dendritic morphology, and axonal projection patterns. Different structures require whole-brain imaging at different resolutions. Thus, it is highly desirable to perform whole-brain imaging at multiple scales. Imaging a complete mammalian brain at synaptic resolution is especially challenging, as it requires continuous imaging from days to weeks because of the large number of voxels to sample, and it is difficult to acquire a constant quality of imaging because of light scattering during in toto imaging. Here, we reveal that light-sheet microscopy has a unique advantage over wide-field microscopy in multi-scale imaging because of its decoupling of illumination and detection. Based on this observation, we have developed a multi-scale light-sheet microscope that combines tiling of light-sheet, automatic zooming, periodic sectioning, and tissue expansion to achieve a constant quality of brain-wide imaging from cellular (3 μm × 3 μm × 8 μm) to sub-micron (0.3 μm × 0.3 μm × 1 μm) spatial resolution rapidly (all within a few hours). We demonstrated the strength of the system by testing it using mouse brains prepared using different clearing approaches. We were able to track electrode tracks as well as axonal projections at sub-micron resolution to trace the full morphology of single medial prefrontal cortex (mPFC) neurons that have remarkable diversity in long-range projections.

scholarly journals Characterization of Scattering Effects in Phantom Samples using Single and Two-Photon Excitation Light Sheet Microscopy

2012 ◽  
Vol 102 (3) ◽  
pp. 195a-196a
Author(s):  
Zeno Lavagnino ◽  
Francesca Cella Zanacchi ◽  
Emiliano Ronzitti ◽  
Ivan Coto Hernandez ◽  
Alberto Diaspro
Keyword(s):  
Light Sheet ◽  
Excitation Light ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Scattering Effects ◽  
Two Photon Excitation
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