scholarly journals Two-photon light-sheet microscopy for high-speed whole-brain functional imaging of zebrafish neuronal physiology and pathology

2020 ◽  
Author(s):  
Giuseppe de Vito ◽  
Lapo Turrini ◽  
Chiara Fornetto ◽  
Pietro Ricci ◽  
Caroline Müllenbroich ◽  
...  
Keyword(s):  
Functional Imaging ◽  
High Speed ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Neuronal Physiology

Whole-brain functional imaging with two-photon light-sheet microscopy

2015 ◽  
Vol 12 (5) ◽  
pp. 379-380 ◽  
Author(s):  
Sébastien Wolf ◽  
Willy Supatto ◽  
Georges Debrégeas ◽  
Pierre Mahou ◽  
Sergei G Kruglik ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy

scholarly journals Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sota Takanezawa ◽  
Takashi Saitou ◽  
Takeshi Imamura
Keyword(s):  
High Speed ◽  
Bessel Beam ◽  
Light Sheet ◽  
Time Lapse ◽  
Whole Body ◽  
Wide Field ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Two Photon Excitation

AbstractTwo-photon excitation can lower phototoxicity and improve penetration depth, but its narrow excitation range restricts its applications in light-sheet microscopy. Here, we propose simple illumination optics, a lens-axicon triplet composed of an axicon and two convex lenses, to generate longer extent Bessel beams. This unit can stretch the beam full width at half maximum of 600–1000 μm with less than a 4-μm waist when using a 10× illumination lens. A two-photon excitation digital scanned light-sheet microscope possessing this range of field of view and ~2–3-μm axial resolution is constructed and used to analyze the cellular dynamics over the whole body of medaka fish. We demonstrate long-term time-lapse observations over several days and high-speed recording with ~3 mm3 volume per 4 s of the embryos. Our system is minimal and suppresses laser power loss, which can broaden applications of two-photon excitation in light-sheet microscopy.

Whole-brain functional imaging at cellular resolution using light-sheet microscopy

2013 ◽  
Vol 10 (5) ◽  
pp. 413-420 ◽  
Author(s):  
Misha B Ahrens ◽  
Michael B Orger ◽  
Drew N Robson ◽  
Jennifer M Li ◽  
Philipp J Keller
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Light Sheet Microscopy ◽  
Cellular Resolution

scholarly journals Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy

2021 ◽  
Author(s):  
Giuseppe de Vito ◽  
Lapo Turrini ◽  
Marie-Caroline Muellenbroich ◽  
pietro ricci ◽  
Giuseppe Sancataldo ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Zebrafish Larvae

scholarly journals Characterization of Scattering Effects in Phantom Samples using Single and Two-Photon Excitation Light Sheet Microscopy

2012 ◽  
Vol 102 (3) ◽  
pp. 195a-196a
Author(s):  
Zeno Lavagnino ◽  
Francesca Cella Zanacchi ◽  
Emiliano Ronzitti ◽  
Ivan Coto Hernandez ◽  
Alberto Diaspro
Keyword(s):  
Light Sheet ◽  
Excitation Light ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Scattering Effects ◽  
Two Photon Excitation

scholarly journals High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Benjamin Schmid ◽  
Gopi Shah ◽  
Nico Scherf ◽  
Michael Weber ◽  
Konstantin Thierbach ◽  
...  
Keyword(s):  
High Speed ◽  
Light Sheet ◽  
Endodermal Cell ◽  
Cell Dynamics ◽  
Light Sheet Microscopy

Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy

2012 ◽  
Vol 9 (7) ◽  
pp. 755-763 ◽  
Author(s):  
Raju Tomer ◽  
Khaled Khairy ◽  
Fernando Amat ◽  
Philipp J Keller
Keyword(s):  
High Speed ◽  
Light Sheet ◽  
High Speed Imaging ◽  
Light Sheet Microscopy

scholarly journals Wide field intravital imaging by two-photon-excitation digital-scanned light-sheet microscopy (2p-DSLM) with a high-pulse energy laser

2014 ◽  
Vol 5 (10) ◽  
pp. 3311 ◽  
Author(s):  
Atsushi Maruyama ◽  
Yusuke Oshima ◽  
Hiroko Kajiura-Kobayashi ◽  
Shigenori Nonaka ◽  
Takeshi Imamura ◽  
...  
Keyword(s):  
Pulse Energy ◽  
Light Sheet ◽  
Intravital Imaging ◽  
Wide Field ◽  
High Pulse Energy ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
High Pulse ◽  
Energy Laser

scholarly journals Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation

2013 ◽  
Vol 21 (11) ◽  
pp. 13824 ◽  
Author(s):  
Florian O. Fahrbach ◽  
Vasily Gurchenkov ◽  
Kevin Alessandri ◽  
Pierre Nassoy ◽  
Alexander Rohrbach
Keyword(s):  
Light Sheet ◽  
Bessel Beams ◽  
Fluorescence Excitation ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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