scholarly journals Multicolor structured illumination microscopyand quantitative control of polychromatic lightwith a digital micromirror device

2021 ◽  
Author(s):  
Peter Brown ◽  
Rory Kruithoff ◽  
Gregory Seedorf ◽  
Douglas Shepherd
Keyword(s):  
Digital Micromirror Device ◽  
Structured Illumination

scholarly journals A Novel Digital-Micromirror Device Based Structured Illumination Microscope for Multicolor Live Cell Imaging

2021 ◽  
Vol 120 (3) ◽  
pp. 179a
Author(s):  
Peter T. Brown ◽  
Rory Kruithoff ◽  
Gregory J. Seedorf ◽  
Douglas P. Shepherd
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Digital Micromirror Device ◽  
Structured Illumination

scholarly journals Fringe optimization for structured illumination super-resolution microscope with digital micromirror device

2019 ◽  
Vol 12 (03) ◽  
pp. 1950014 ◽  
Author(s):  
Xibin Yang ◽  
Qian Zhu ◽  
Zhenglong Sun ◽  
Gang Wen ◽  
Xin Jin ◽  
...  
Keyword(s):  
Modulation Depth ◽  
Low Cost ◽  
Super Resolution ◽  
Fluorescent Labeling ◽  
Live Cells ◽  
Digital Micromirror Device ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Wide Range ◽  
Fringe Patterns

Structured illumination microscopy (SIM) is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly used fluorescent labeling methods. Structured illumination can be obtained by either laser interference or projection of fringe patterns. Here, we proposed a fringe projector composed of a compact multi-wavelength LEDs module and a digital micromirror device (DMD) which can be directly attached to most commercial inverted fluorescent microscopes and update it into a SIM system. The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured light field were studied. With the optimized fringe pattern, [Formula: see text] resolution improvement could be obtained with high-end oil objectives. Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated. Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in the field of life science and medicine.

scholarly journals Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device

2014 ◽  
Vol 5 (8) ◽  
pp. 2526 ◽  
Author(s):  
Li-Chung Cheng ◽  
Chi-Hsiang Lien ◽  
Yong Da Sie ◽  
Yvonne Yuling Hu ◽  
Chun-Yu Lin ◽  
...  
Keyword(s):  
Digital Micromirror Device ◽  
Structured Illumination ◽  
Multiphoton Excitation ◽  
Temporal Focusing

MULTIPLE SCATTERING SUPPRESSION IN PLANAR LASER IMAGING OF DENSE SPRAYS BY MEANS OF STRUCTURED ILLUMINATION

2010 ◽  
Vol 20 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Edouard Berrocal ◽  
Elias Kristensson ◽  
Mattias Richter ◽  
Mark Linne ◽  
Marcus Alden
Keyword(s):  
Multiple Scattering ◽  
Structured Illumination ◽  
Laser Imaging ◽  
Planar Laser
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