scholarly journals Dual GRIN lens two-photon endoscopy for high-speed volumetric and deep brain imaging

2020 ◽  
Vol 12 (1) ◽  
pp. 162
Author(s):  
Yu-Feng Chien ◽  
Jyun-Yi Lin ◽  
Po-Ting Yeh ◽  
Kuo-Jen Hsu ◽  
Yu-Hsuan Tsai ◽  
...  
Keyword(s):  
Brain Imaging ◽  
High Speed ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

scholarly journals Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes

2020 ◽  
Vol 6 (40) ◽  
pp. eabc6521 ◽  
Author(s):  
Zhongya Qin ◽  
Congping Chen ◽  
Sicong He ◽  
Ye Wang ◽  
Kam Fai Tam ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Adaptive Optics ◽  
Critical Role ◽  
Random Access ◽  
Brain Structures ◽  
Invasive Approach ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

Optical deep-brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep-brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

scholarly journals Adaptive optics two-photon endomicroscopy enables deep brain imaging at synaptic resolution over large volumes

2020 ◽  
Author(s):  
Zhongya Qin ◽  
Congping Chen ◽  
Sicong He ◽  
Ye Wang ◽  
Kam Fai Tam ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Adaptive Optics ◽  
Critical Role ◽  
Random Access ◽  
Brain Structures ◽  
Invasive Approach ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

AbstractOptical deep brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on the direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

scholarly journals Dual GRIN lens two-photon endoscopy for high-speed volumetric and deep brain imaging

2020 ◽  
Author(s):  
Yu-Feng Chien ◽  
Jyun-Yi Lin ◽  
Po-Ting Yeh ◽  
Kuo-Jen Hsu ◽  
Yu-Hsuan Tsai ◽  
...  
Keyword(s):  
Penetration Depth ◽  
High Speed ◽  
Gradient Index ◽  
Spatiotemporal Resolution ◽  
Deep Tissue ◽  
Volumetric Imaging ◽  
Two Photon ◽  
Brain Functions ◽  
Grin Lens ◽  
Deep Brain

AbstractStudying neural connections and activities in vivo is fundamental to understanding brain functions. Given the cm-size brain and three-dimensional neural circuit dynamics, deep-tissue, high-speed volumetric imaging is highly desirable for brain study. With sub-micrometer spatial resolution, intrinsic optical sectioning, and deep-tissue penetration capability, two-photon microscopy (2PM) has found a niche in neuroscience. However, current 2PM typically relies on slow axial scan for volumetric imaging, and the maximal penetration depth is only about 1 mm. Here, we demonstrate that by integrating two gradient-index (GRIN) lenses into 2PM, both penetration depth and volume-imaging rate can be significantly improved. Specifically, an 8-mm long GRIN lens allows imaging relay through a whole mouse brain, while a tunable acoustic gradient-index (TAG) lens provides sub-second volume rate via 100 kHz ∼ 1 MHz axial scan. This technique enables the study of calcium dynamics in cm-deep brain regions with sub-cellular and sub-second spatiotemporal resolution, paving the way for interrogating deep-brain functional connectome.

Ultrafast two-photon microscopy for high-speed brain imaging in awake mice (Conference Presentation)

2018 ◽  
Author(s):  
Radoslaw Chrapkiewicz ◽  
Tong Zhang ◽  
Oscar Hernandez ◽  
Adam S. Shai ◽  
Mark J. Wagner ◽  
...  
Keyword(s):  
Brain Imaging ◽  
High Speed ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Theranostic OCT microneedle for fast ultrahigh-resolution deep-brain imaging and efficient laser ablation in vivo

2020 ◽  
Vol 6 (15) ◽  
pp. eaaz9664 ◽  
Author(s):  
Wu Yuan ◽  
Defu Chen ◽  
Rachel Sarabia-Estrada ◽  
Hugo Guerrero-Cázares ◽  
Dawei Li ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Brain Imaging ◽  
Mouse Brain ◽  
High Speed ◽  
Continuous Wave ◽  
Brain Diseases ◽  
Outer Diameter ◽  
Ultrahigh Resolution ◽  
Deep Brain

Current minimally invasive optical techniques for in vivo deep-brain imaging provide a limited resolution, field of view, and speed. These limitations prohibit direct assessment of detailed histomorphology of various deep-seated brain diseases at their native state and therefore hinder the potential clinical utilities of those techniques. Here, we report an ultracompact (580 μm in outer diameter) theranostic deep-brain microneedle combining 800-nm optical coherence tomography imaging with laser ablation. Its performance was demonstrated by in vivo ultrahigh-resolution (1.7 μm axial and 5.7 μm transverse), high-speed (20 frames per second) volumetric imaging of mouse brain microstructures and optical attenuation coefficients. Its translational potential was further demonstrated by in vivo cancer visualization (with an imaging depth of 1.23 mm) and efficient tissue ablation (with a 1448-nm continuous-wave laser at a 350-mW power) in a deep mouse brain (with an ablation depth of about 600 μm).

Integrated Fluorescent Nanoprobe Design for High‐Speed In Vivo Two‐Photon Microscopic Imaging of Deep‐Brain Vasculature in Mice

2021 ◽  
pp. 2010698
Author(s):  
Minami Takezaki ◽  
Ryosuke Kawakami ◽  
Shozo Onishi ◽  
Yasutaka Suzuki ◽  
Jun Kawamata ◽  
...  
Keyword(s):  
High Speed ◽  
Microscopic Imaging ◽  
Two Photon ◽  
Fluorescent Nanoprobe ◽  
Brain Vasculature ◽  
Deep Brain

Adaptive optics two-photon microendoscopy for high-resolution and deep-brain imaging in vivo

2020 ◽  
Author(s):  
Congping Chen ◽  
Zhongya Qin ◽  
Sicong He ◽  
Wanjie Wu ◽  
Ye Wang ◽  
...  
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Adaptive Optics ◽  
Two Photon ◽  
Deep Brain

GRIN lens based high speed confocal system for deep brain calcium imaging

2017 ◽  
Author(s):  
Zhongyang Qi ◽  
Qingchun Guo ◽  
Qian Liu ◽  
Min Chen ◽  
Hui Gong ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
High Speed ◽  
Grin Lens ◽  
Deep Brain

scholarly journals Deformable mirror-based two-photon microscopy for axial mammalian brain imaging

2019 ◽  
Author(s):  
Alba Peinado ◽  
Eduardo Bendek ◽  
Sae Yokoyama ◽  
Kira E. Poskanzer
Keyword(s):  
Brain Imaging ◽  
High Speed ◽  
Brain Slices ◽  
Axial Position ◽  
Mammalian Brain ◽  
Deformable Mirror ◽  
Radius Of Curvature ◽  
Step Size ◽  
Two Photon

AbstractThis work presents the design and implementation of an enhanced version of a traditional two-photon (2P) microscope with the addition of high-speed axial scanning for live mammalian brain imaging. Our implementation utilizes a deformable mirror (DM) that can rapidly apply different defocus shapes to manipulate the laser beam divergence and consequently control the axial position of the beam focus in the sample. We provide a mathematical model describing the DM curvature, then experimentally characterize the radius of curvature as well as the Zernike terms of the DM surface for a given set of defocuses. A description of the optical setup of the 2P microscope is detailed. We conduct a thorough calibration of the system, determining the point spread function, the total scanning range, the axial step size, and the intensity curvature as a function of depth. Finally, the instrument is used for imaging different neurobiological samples, including fixed brain slices and in vivo mouse cerebral cortex.

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