Adaptive optics two-photon microendoscopy for high-resolution and deep-brain imaging in vivo

2020 ◽  
Author(s):  
Congping Chen ◽  
Zhongya Qin ◽  
Sicong He ◽  
Wanjie Wu ◽  
Ye Wang ◽  
...  
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Adaptive Optics ◽  
Two Photon ◽  
Deep Brain

scholarly journals Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes

2020 ◽  
Vol 6 (40) ◽  
pp. eabc6521 ◽  
Author(s):  
Zhongya Qin ◽  
Congping Chen ◽  
Sicong He ◽  
Ye Wang ◽  
Kam Fai Tam ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Adaptive Optics ◽  
Critical Role ◽  
Random Access ◽  
Brain Structures ◽  
Invasive Approach ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

Optical deep-brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep-brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

scholarly journals Adaptive optics two-photon endomicroscopy enables deep brain imaging at synaptic resolution over large volumes

2020 ◽  
Author(s):  
Zhongya Qin ◽  
Congping Chen ◽  
Sicong He ◽  
Ye Wang ◽  
Kam Fai Tam ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Adaptive Optics ◽  
Critical Role ◽  
Random Access ◽  
Brain Structures ◽  
Invasive Approach ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

AbstractOptical deep brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on the direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

scholarly journals High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy

2021 ◽  
Author(s):  
Lina Streich ◽  
Juan Boffi ◽  
Ling Wang ◽  
Khaleel Alhalaseh ◽  
Matteo Barbieri ◽  
...  
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Adaptive Optics ◽  
Deep Brain

Bright AIEgen–Protein Hybrid Nanocomposite for Deep and High‐Resolution In Vivo Two‐Photon Brain Imaging

2019 ◽  
Vol 29 (29) ◽  
pp. 1902717 ◽  
Author(s):  
Shaowei Wang ◽  
Fang Hu ◽  
Yutong Pan ◽  
Lai Guan Ng ◽  
Bin Liu
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Hybrid Nanocomposite ◽  
Two Photon

scholarly journals Minimally invasive deep-brain imaging through a 50 μm-core multimode fibre

2018 ◽  
Author(s):  
Sebastian A. Vasquez-Lopez ◽  
Vadim Koren ◽  
Martin Plöschner ◽  
Zahid Padamsey ◽  
Tomáš Čižmár ◽  
...  
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Light Propagation ◽  
Neural Circuit ◽  
Complex Refractive Index ◽  
Brain Structures ◽  
Brain Regions ◽  
Long Distance ◽  
Deep Brain

AbstractAchieving optical access to deep-brain structures represents an important step towards the goal of understanding the mammalian central nervous system. The complex refractive index distribution within brain tissue introduces severe aberrations to long-distance light propagation thereby prohibiting image reconstruction using currently available non-invasive techniques. In an attempt to overcome this challenge endoscopic approaches have been adopted, principally in the form of fibre bundles or GRIN-lens based endoscopes. Unfortunately, these approaches create substantial mechanical lesions of the tissue precipitating neuropathological responses that include inflammation and gliosis. Together, lesions and the associated neuropathology may compromise neural circuit performance. By replacing Fourier-based image relay with a holographic approach, we have been able to reduce the volume of tissue lesion by more than 100-fold, while preserving diffraction-limited imaging performance. Here we demonstrate high-resolution fluorescence imaging of neuronal structures, dendrites and synaptic specialisations, in deep-brain regions of living mice. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

scholarly journals High-resolution two-photon transcranial imaging of brain using direct wavefront sensing

2020 ◽  
Author(s):  
Congping Chen ◽  
Zhongya Qin ◽  
Sicong He ◽  
Shaojun Liu ◽  
Shun-Fat Lau ◽  
...  
Keyword(s):  
High Resolution ◽  
Adaptive Optics ◽  
Pyramidal Neurons ◽  
Imaging System ◽  
Superficial Layer ◽  
Wavefront Sensing ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Transcranial Imaging

AbstractImaging of the brain in its native state at high resolution poses major challenges to visualization techniques. Two-photon microscopy integrated with the thinned-skull or optical clearing skull technique provides a minimally invasive tool for in vivo imaging of the cortex of mice without activating immune response and inducing brain injury. However, the imaging contrast and resolution are severely compromised by the optical heterogeneity of the skull, limiting the imaging depth to the superficial layer. Here, we develop adaptive optics two-photon microscopy for high-resolution transcranial imaging of layer 5 pyramidal neurons up to 700 μm below pia in living mice. In particular, an optimized configuration of imaging system and new wavefront sensing algorithm are proposed for accurate correction for the aberrations induced by the skull window and brain tissue. We investigated microglia-plaque interaction in living brain of Alzheimer’s disease and demonstrated high-precision laser dendrotomy and single-spine ablation.

scholarly journals High-Resolution Adaptive Optics in Vivo Autofluorescence Imaging in Stargardt Disease

2019 ◽  
Vol 137 (6) ◽  
pp. 603 ◽  
Author(s):  
Hongxin Song ◽  
Ethan A. Rossi ◽  
Qiang Yang ◽  
Charles E. Granger ◽  
Lisa R. Latchney ◽  
...  
Keyword(s):  
High Resolution ◽  
Adaptive Optics ◽  
Autofluorescence Imaging ◽  
Stargardt Disease

scholarly journals Ultra-high resolution adaptive optics: optical coherence tomography for in vivo imaging of healthy and diseased retinal structures

2008 ◽  
Author(s):  
Robert J. Zawadzki ◽  
Yan Zhang ◽  
Steven M. Jones ◽  
Stacey S. Choi ◽  
Barry Cense ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Optical Coherence

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

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